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. 1994 Mar;68(3):1516–1521. doi: 10.1128/jvi.68.3.1516-1521.1994

Spontaneous development of anti-hepatitis B virus envelope (anti-idiotypic) antibodies in animals immunized with human liver endonexin II or with the F(ab')2 fragment of anti-human liver endonexin II immunoglobulin G: evidence for a receptor-ligand-like relationship between small hepatitis B surface antigen and endonexin II.

K Hertogs 1, E Depla 1, T Crabbé 1, W De Bruin 1, W Leenders 1, H Moshage 1, S H Yap 1
PMCID: PMC236608  PMID: 8107214

Abstract

In a previous study, we have identified endonexin II (E-II) on human liver plasma membranes as a specific, Ca(2+)-dependent, small hepatitis B surface antigen (HBsAg)-binding protein. In this article, we describe the spontaneous development of anti-HBs antibodies in rabbits immunized with native or recombinant human liver E-II and in chickens immunized with the F(ab')2 fragment of rabbit anti-human liver E-II immunoglobulin G. Anti-HBs activity was not observed in rabbits immunized with rat liver E-II. Cross-reactivity of anti-E-II antibodies to HBsAg epitopes was excluded, since anti-HBs and anti-E-II activities can be separated by E-II affinity chromatography. The existence of an anti-idiotypic antibody is further demonstrated by competitive binding of human liver E-II and this antibody (Ab2) to small HBsAg, suggesting that Ab2 mimics a specific E-II epitope that interacts with small HBsAg. In addition, it was demonstrated that anti-HBs antibodies developed in rabbits after immunization with intact human liver E-II or in chickens after immunization with F(ab')2 fragments of rabbit anti-human liver E-II immunoglobulin G recognize the same epitopes on small HBsAg. These findings strongly indicate that human liver E-II is a very specific small HBsAg-binding protein and support the assumption that human liver E-II is the hepatitis B virus receptor protein.

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Selected References

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  1. Carman W. F., Zanetti A. R., Karayiannis P., Waters J., Manzillo G., Tanzi E., Zuckerman A. J., Thomas H. C. Vaccine-induced escape mutant of hepatitis B virus. Lancet. 1990 Aug 11;336(8711):325–329. doi: 10.1016/0140-6736(90)91874-a. [DOI] [PubMed] [Google Scholar]
  2. Dash S., Rao K. V., Joshi B., Nayak N. C., Panda S. K. Significance of natural polymerized albumin and its receptor in hepatitis B infection of hepatocytes. Hepatology. 1991 Jan;13(1):134–142. [PubMed] [Google Scholar]
  3. Dash S., Rao K. V., Panda S. K. Receptor for pre-S1(21-47) component of hepatitis B virus on the liver cell: role in virus cell interaction. J Med Virol. 1992 Jun;37(2):116–121. doi: 10.1002/jmv.1890370208. [DOI] [PubMed] [Google Scholar]
  4. Ganem D. Persistent infection of humans with hepatitis B virus: mechanisms and consequences. Rev Infect Dis. 1982 Sep-Oct;4(5):1026–1047. doi: 10.1093/clinids/4.5.1026. [DOI] [PubMed] [Google Scholar]
  5. Ganem D., Varmus H. E. The molecular biology of the hepatitis B viruses. Annu Rev Biochem. 1987;56:651–693. doi: 10.1146/annurev.bi.56.070187.003251. [DOI] [PubMed] [Google Scholar]
  6. Gerlich W. H., Lu X., Heermann K. H. Studies on the attachment and penetration of hepatitis B virus. J Hepatol. 1993;17 (Suppl 3):S10–S14. doi: 10.1016/s0168-8278(05)80417-9. [DOI] [PubMed] [Google Scholar]
  7. Hertogs K., Leenders W. P., Depla E., De Bruin W. C., Meheus L., Raymackers J., Moshage H., Yap S. H. Endonexin II, present on human liver plasma membranes, is a specific binding protein of small hepatitis B virus (HBV) envelope protein. Virology. 1993 Dec;197(2):549–557. doi: 10.1006/viro.1993.1628. [DOI] [PubMed] [Google Scholar]
  8. Iwarson S., Tabor E., Thomas H. C., Goodall A., Waters J., Snoy P., Shih J. W., Gerety R. J. Neutralization of hepatitis B virus infectivity by a murine monoclonal antibody: an experimental study in the chimpanzee. J Med Virol. 1985 May;16(1):89–96. doi: 10.1002/jmv.1890160112. [DOI] [PubMed] [Google Scholar]
  9. Jensenius J. C., Andersen I., Hau J., Crone M., Koch C. Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG. J Immunol Methods. 1981;46(1):63–68. doi: 10.1016/0022-1759(81)90333-1. [DOI] [PubMed] [Google Scholar]
  10. Kaiser J., Stockert R. J., Schein P. S., Wolkoff A. W. Recognition of hepatitis B surface antigen by the human hepatoma cell line HepG2. Trans Assoc Am Physicians. 1986;99:86–91. [PubMed] [Google Scholar]
  11. Krone B., Lenz A., Heermann K. H., Seifer M., Lu X. Y., Gerlich W. H. Interaction between hepatitis B surface proteins and monomeric human serum albumin. Hepatology. 1990 Jun;11(6):1050–1056. doi: 10.1002/hep.1840110622. [DOI] [PubMed] [Google Scholar]
  12. Lee S. W., Wulfkuhle J. D., Otto J. J. Vinculin binding site mapped on talin with an anti-idiotypic antibody. J Biol Chem. 1992 Aug 15;267(23):16355–16358. [PubMed] [Google Scholar]
  13. Leenders W. P., Glansbeek H. L., de Bruin W. C., Yap S. H. Binding of the major and large HBsAg to human hepatocytes and liver plasma membranes: putative external and internal receptors for infection and secretion of hepatitis B virus. Hepatology. 1990 Jul;12(1):141–147. doi: 10.1002/hep.1840120122. [DOI] [PubMed] [Google Scholar]
  14. Leenders W. P., Hertogs K., Moshage H., Yap S. H. Host and tissue tropism of hepatitis B virus. Liver. 1992 Apr;12(2):51–55. doi: 10.1111/j.1600-0676.1992.tb00556.x. [DOI] [PubMed] [Google Scholar]
  15. Machida A., Kishimoto S., Ohnuma H., Baba K., Ito Y., Miyamoto H., Funatsu G., Oda K., Usuda S., Togami S. A polypeptide containing 55 amino acid residues coded by the pre-S region of hepatitis B virus deoxyribonucleic acid bears the receptor for polymerized human as well as chimpanzee albumins. Gastroenterology. 1984 May;86(5 Pt 1):910–918. [PubMed] [Google Scholar]
  16. McAleer W. J., Buynak E. B., Maigetter R. Z., Wampler D. E., Miller W. J., Hilleman M. R. Human hepatitis B vaccine from recombinant yeast. Nature. 1984 Jan 12;307(5947):178–180. doi: 10.1038/307178a0. [DOI] [PubMed] [Google Scholar]
  17. Neurath A. R., Kent S. B., Strick N., Parker K. Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell. 1986 Aug 1;46(3):429–436. doi: 10.1016/0092-8674(86)90663-x. [DOI] [PubMed] [Google Scholar]
  18. Neurath A. R., Strick N., Sproul P., Ralph H. E., Valinsky J. Detection of receptors for hepatitis B virus on cells of extrahepatic origin. Virology. 1990 Jun;176(2):448–457. doi: 10.1016/0042-6822(90)90014-i. [DOI] [PubMed] [Google Scholar]
  19. Pepinsky R. B., Tizard R., Mattaliano R. J., Sinclair L. K., Miller G. T., Browning J. L., Chow E. P., Burne C., Huang K. S., Pratt D. Five distinct calcium and phospholipid binding proteins share homology with lipocortin I. J Biol Chem. 1988 Aug 5;263(22):10799–10811. [PubMed] [Google Scholar]
  20. Petit M. A., Capel F., Dubanchet S., Mabit H. PreS1-specific binding proteins as potential receptors for hepatitis B virus in human hepatocytes. Virology. 1992 Mar;187(1):211–222. doi: 10.1016/0042-6822(92)90309-d. [DOI] [PubMed] [Google Scholar]
  21. Petit M. A., Dubanchet S., Capel F., Voet P., Dauguet C., Hauser P. HepG2 cell binding activities of different hepatitis B virus isolates: inhibitory effect of anti-HBs and anti-preS1(21-47). Virology. 1991 Feb;180(2):483–491. doi: 10.1016/0042-6822(91)90062-g. [DOI] [PubMed] [Google Scholar]
  22. Pontisso P., Petit M. A., Bankowski M. J., Peeples M. E. Human liver plasma membranes contain receptors for the hepatitis B virus pre-S1 region and, via polymerized human serum albumin, for the pre-S2 region. J Virol. 1989 May;63(5):1981–1988. doi: 10.1128/jvi.63.5.1981-1988.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Salacinski P. R., McLean C., Sykes J. E., Clement-Jones V. V., Lowry P. J. Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen). Anal Biochem. 1981 Oct;117(1):136–146. doi: 10.1016/0003-2697(81)90703-x. [DOI] [PubMed] [Google Scholar]
  24. Waters J. A., Kennedy M., Voet P., Hauser P., Petre J., Carman W., Thomas H. C. Loss of the common "A" determinant of hepatitis B surface antigen by a vaccine-induced escape mutant. J Clin Invest. 1992 Dec;90(6):2543–2547. doi: 10.1172/JCI116148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Waters J., Pignatelli M., Galpin S., Ishihara K., Thomas H. C. Virus-neutralizing antibodies to hepatitis B virus: the nature of an immunogenic epitope on the S gene peptide. J Gen Virol. 1986 Nov;67(Pt 11):2467–2473. doi: 10.1099/0022-1317-67-11-2467. [DOI] [PubMed] [Google Scholar]
  26. Yu M. W., Finlayson J. S., Shih J. W. Interaction between various polymerized human albumins and hepatitis B surface antigen. J Virol. 1985 Sep;55(3):736–743. doi: 10.1128/jvi.55.3.736-743.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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