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. 1994 Mar;68(3):1675–1681. doi: 10.1128/jvi.68.3.1675-1681.1994

Mutations which affect the inhibition of protein phosphatase 2A by simian virus 40 small-t antigen in vitro decrease viral transformation.

S Mungre 1, K Enderle 1, B Turk 1, A Porrás 1, Y Q Wu 1, M C Mumby 1, K Rundell 1
PMCID: PMC236626  PMID: 8107228

Abstract

Three independent point mutations within residues 97 to 103 of the simian virus 40-small-t antigen (small-t) greatly reduced the ability of purified small-t to inhibit protein phosphatase 2A in vitro. These mutations affected the interaction of small-t antigen with the protein phosphatase 2A A subunit translated in vitro, and a peptide from the region identified by these mutations released the A subunit from immune complexes. When introduced into virus, the mutations eliminated the ability of small-t to enhance viral transformation of growth-arrested rat F111 cells. In contrast, the mutant small-t antigens were unimpaired in the transactivation of the adenovirus E2 promoter, an activity which was reduced by a double mutation in small-t residues 43 and 45.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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