Abstract
Precise measurement of the parasite load in helminthic infections makes it possible to correlate morbidity, the individual response to treatment, and the best control methods. A membrane filtration technique was recently developed for quantifying Wuchereria bancrofti microfilaraemia. The present paper describes its application to onchocerciasis in a study on 107 patients in northern Togo. The technique is cheap and simple to apply under field conditions, and is extremely efficient at recovering microfilariae from skin snips.
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Selected References
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- Anderson J., Fuglsang H., Hamilton P. J., de Marshall T. F. The prognostic value of head nodules and microfilariae in the skin in relation to ocular onchocerciasis. Tropenmed Parasitol. 1975 Jun;26(2):191–195. [PubMed] [Google Scholar]
- Bell D. Membrane filters and microfilariae: a new diagnostic technique. Ann Trop Med Parasitol. 1967 Jun;61(2):220–223. doi: 10.1080/00034983.1967.11720805. [DOI] [PubMed] [Google Scholar]
- Bryan J. H., Southgate B. A. Some observations on filariasis in Western Samoa after mass administration of diethylcarbamazine. Trans R Soc Trop Med Hyg. 1976;70(1):39–48. doi: 10.1016/0035-9203(76)90005-5. [DOI] [PubMed] [Google Scholar]
- Chularerk P., Desowitz R. S. A simplified membrane filtration technique for the diagnosis of microfilaremia. J Parasitol. 1970 Jun;56(3):623–624. [PubMed] [Google Scholar]
- Desowitz R. S., Southgate B. A., Mataika J. U. Studies on filariasis in the Pacific. 3. Comparative efficacy of the stained blood-film, counting-chamber and membrane-filtration techniques for the diagnosis of Wuchereria bancrofti microfilaraemia in untreated patients in areas of low endemicity. Southeast Asian J Trop Med Public Health. 1973 Sep;4(3):329–335. [PubMed] [Google Scholar]
- Desowitz R. S., Southgate B. A. Studies of filariasis in the Pacific. 2. The persistence of microfilaraemia in diethylcarbamazine treated populations of Fiji and Western Samoa: diagnostic application of the membrane-filtration technique. Southeast Asian J Trop Med Public Health. 1973 Jun;4(2):179–183. [PubMed] [Google Scholar]
- Duke B. O., Anderson J., Fuglsang H. The Onchocerca volvulus transmission potentials and associated patterns of onchocerciasis at four Cameroon Sudan-savanna villages. Tropenmed Parasitol. 1975 Jun;26(2):143–154. [PubMed] [Google Scholar]
- Duke B. O., Scheffel P. D., Guyon J., Moore P. J. The concentration of Onchocerca volvulus microfilariae in skin snips taken over twenty-four hours. Ann Trop Med Parasitol. 1967 Jun;61(2):206–219. doi: 10.1080/00034983.1967.11686480. [DOI] [PubMed] [Google Scholar]
- Duke B. O. The effects of drugs on Onchocerca volvulus. 1. Methods of assessment, population dynamics of the parasite and the effects of diethylcarbamazine. Bull World Health Organ. 1968;39(2):137–146. [PMC free article] [PubMed] [Google Scholar]
- Picq J. J., Coz J., Jardel J. P. Une methode d'evaluation des densites microfilariennes d'Onchocerca volvulus Leuckart, 1893 chez des onchocerquiens: technique et temps de lecture des biopsies cutanees. Bull World Health Organ. 1971;45(4):517–520. [PMC free article] [PubMed] [Google Scholar]
- Picq J. J., Jardel J. P. Une méthode d'évaluation des densités microfilariennes d'Onchocerca volvulus Leuckart, 1893 chez des onchocerquiens. Répartition des densités microfilariennes suivant les sites et niveaux de prélèvement des biopsies cutanées; variations des densités microfilariennes au cours des 24 heures. Bull World Health Organ. 1974;51(2):145–153. [PMC free article] [PubMed] [Google Scholar]
- Southgate B. A. A quantitative approach to parasitological techniques in Bancroftian filariasis and its effect on epidemiological understanding. Trans R Soc Trop Med Hyg. 1974;68(3):177–186. doi: 10.1016/0035-9203(74)90113-8. [DOI] [PubMed] [Google Scholar]