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. 1976;53(4):399–406.

Parasite cultivation in relation to research on the chemotherapy of malaria

P I Trigg
PMCID: PMC2366511  PMID: 1086733

Abstract

Attempts to develop techniques for the continuous in vitro cultivation of the malaria parasite have not yet proved successful. It has not been possible to obtain the complete sporogonic development of the parasite in vitro although some progress was made with Plasmodium relictum and P. berghei. Exoerythrocytic stages of P. gallinaceum have been successfully cultivated in vitro in tissue explants and those of P. fallax have been grown in turkey primary embryo tissue cells. With the recent development of mammalian liver cell lines, prospects for the in vitro cultivation of exoerythrocytic stages of mammalian plasmodia are greatly improved. While it is still not possible to cultivate erythrocytic stages of plasmodia serially in vitro some species have been successfully grown through one asexual cycle. This progress has led to a number of applications of parasite cultivation to chemotherapeutic studies, to the testing of new antimalarial drugs, and especially to the testing of the susceptibility of P. falciparum to chloroquine. Cultivation technique is greatly improved by an appropriate choice of culture media. The addition of fresh red cells to the subculture system permits relatively high rates of invasion and multiplication of the parasite to be obtained. As well as its application in the screening and evaluation of antimalarial compounds, the in vitro cultivation technique is also very suitable for studying the entry mechanism of the parasite into red blood cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aikawa M., Beaudoin R. L. Morphological effects of 8-aminoquinolines on the exoerythrocytic stages of Plasmodium fallax. Mil Med. 1969 Sep;134(10):986–999. [PubMed] [Google Scholar]
  2. Alger N. E. In vitro development of Plasmodium berghei ookinetes. Nature. 1968 May 25;218(5143):774–774. doi: 10.1038/218774a0. [DOI] [PubMed] [Google Scholar]
  3. Bannister L. H., Butcher G. A., Dennis E. D., Mitchell G. H. Structure and invasive behaviour of Plasmodium knowlesi merozoites in vitro. Parasitology. 1975 Dec;71(3):483–491. doi: 10.1017/s0031182000047247. [DOI] [PubMed] [Google Scholar]
  4. Bass C. C., Johns F. M. THE CULTIVATION OF MALARIAL PLASMODIA (PLASMODIUM VIVAX AND PLASMODIUM FALCIPARUM) IN VITRO. J Exp Med. 1912 Oct 1;16(4):567–579. doi: 10.1084/jem.16.4.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beaudoin R. L., Aikawa M. Primaquine-induced changes in morphology of exoerythrocytic stages of malaria. Science. 1968 Jun 14;160(3833):1233–1234. doi: 10.1126/science.160.3833.1233. [DOI] [PubMed] [Google Scholar]
  6. Beaudoin R. L., Strome C. P., Clutter W. G. A tissue culture system for the study of drug action against the tissue phase of malaria. Mil Med. 1969 Sep;134(10):979–985. [PubMed] [Google Scholar]
  7. Bertagna P., Cohen S., Geiman Q. M., Haworth J., Koenigk E., Richards W. H., Trigg P. I. Cultivation techniques for the erythrocytic stages of malaria parasites. Bull World Health Organ. 1972;47(3):357–373. [PMC free article] [PubMed] [Google Scholar]
  8. Butcher G. A., Cohen S. Short-term culture of Plasmodium knowlesi. Parasitology. 1971 Apr;62(2):309–320. doi: 10.1017/s0031182000071547. [DOI] [PubMed] [Google Scholar]
  9. Eagle H. Buffer combinations for mammalian cell culture. Science. 1971 Oct 29;174(4008):500–503. doi: 10.1126/science.174.4008.500. [DOI] [PubMed] [Google Scholar]
  10. Geiman Q. M., Siddigui W. A., Schnell J. V. In vitro studies on erythrocytic stages of plasmodia; medium improvement and results with seven species of malarial parasites. Mil Med. 1966 Sep;131(9 Suppl):1015–1025. [PubMed] [Google Scholar]
  11. Gerschenson L. E., Andersson M., Molson J., Okigaki T. Tyrosine transaminase induction by dexamethasone in a new rat liver cell line. Science. 1970 Nov 20;170(3960):859–861. doi: 10.1126/science.170.3960.859. [DOI] [PubMed] [Google Scholar]
  12. Gutteridge W. E., Trigg P. I. Action of pyrimethamine and related drugs against Plasmodium knowlesi in vitr. Parasitology. 1971 Jun;62(3):431–444. doi: 10.1017/s0031182000077581. [DOI] [PubMed] [Google Scholar]
  13. Gutteridge W. E., Trigg P. I. Incorporation of radioactive precursors into DNA and RNA of Plasmodium knowlesi in vitro. J Protozool. 1970 Feb;17(1):89–96. doi: 10.1111/j.1550-7408.1970.tb05163.x. [DOI] [PubMed] [Google Scholar]
  14. Lambiotte M., Susor W. A., Cahn R. D. Morphological and biochemical observations on mammalian liver cells in culture. Isolation of a clonal strain from rat liver. Biochimie. 1972;54(9):1179–1187. doi: 10.1016/s0300-9084(72)80022-1. [DOI] [PubMed] [Google Scholar]
  15. Peters W., Seaton D. R. Sensitivity of Plasmodium falciparum to chloroquine in Africa. Ann Trop Med Parasitol. 1971 Jun;65(2):267–269. doi: 10.1080/00034983.1971.11686753. [DOI] [PubMed] [Google Scholar]
  16. Phillips R. S., Trigg P. I., Scott-Finnigan T. J., Bartholomew R. K. Culture of Plasmodium falciparum in vitro: a subculture technique used for demonstrating antiplasmodial activity in serum from some Gambians, resident in an endemic malarious area. Parasitology. 1972 Dec;65(3):525–535. doi: 10.1017/s0031182000044139. [DOI] [PubMed] [Google Scholar]
  17. Polet H., Barr C. F. Chloroquine and dihydroquinine. In vitro studies by their antimalarial effect upon Plasmodium knowlesi. J Pharmacol Exp Ther. 1968 Dec;164(2):380–386. [PubMed] [Google Scholar]
  18. Powell R. D., Berglund E. M. Effects of chloroquine upon the maturation of asexual erythrocytic forms of Plasmodium vivax in vitro. Am J Trop Med Hyg. 1974 Nov;23(6):1007–1014. doi: 10.4269/ajtmh.1974.23.1007. [DOI] [PubMed] [Google Scholar]
  19. Richards W. H., Williams S. G. Malaria in studies in vitro. III: the protein synthesising activity of Plasmodium falciparum in vitro after drug treatment in vivo. Ann Trop Med Parasitol. 1975 Jun;69(2):135–140. [PubMed] [Google Scholar]
  20. Richards W. H., Williams S. G. Malaria studies in vitro. II. The measurement of drug activities using leucocyte-free blood-dilution cultures of Plasmodium berghei and 3H-leucine. Ann Trop Med Parasitol. 1973 Jun;67(2):179–190. [PubMed] [Google Scholar]
  21. Rosales-Ronquillo M. C., Nienaber G., Silverman P. H. Plasmodium berghei ookinete formation in a nonvector cell line. J Parasitol. 1974 Dec;60(6):1039–1040. [PubMed] [Google Scholar]
  22. Siddiqui W. A., Schnell J. V. Use of various buffers for in vitro cultivation of malarial parasites. J Parasitol. 1973 Jun;59(3):516–519. [PubMed] [Google Scholar]
  23. Smalley M. E., Butcher G. A. The in vitro culture of the blood stages of Plasmodium berghei. Int J Parasitol. 1975 Apr;5(2):131–132. doi: 10.1016/0020-7519(75)90018-1. [DOI] [PubMed] [Google Scholar]
  24. Sucharit P., Harinasuta T., Kasemsuth R., Chongsuphajaisiddhi T., Tongprasroeth N. Preliminary report of in vitro and in vivo studies on Falciparum malaria in Thailand. Southeast Asian J Trop Med Public Health. 1974 Sep;5(3):460–461. [PubMed] [Google Scholar]
  25. Swetly P., Ostertag W. Friend virus release and induction of haemoglobin synthesis in erythroleukaemic cells respond differently to interferon. Nature. 1974 Oct 18;251(5476):642–644. doi: 10.1038/251642a0. [DOI] [PubMed] [Google Scholar]
  26. Trigg P. I. Invasion of erythrocytes by Plasmodium falciparum in vitro. Parasitology. 1975 Dec;71(3):433–436. doi: 10.1017/s0031182000047193. [DOI] [PubMed] [Google Scholar]
  27. Vanderberg J. P. Studies on the motility of Plasmodium sporozoites. J Protozool. 1974 Oct;21(4):527–537. doi: 10.1111/j.1550-7408.1974.tb03693.x. [DOI] [PubMed] [Google Scholar]
  28. Walsh C. J., Sherman I. W. Purine and pyrimidine synthesis by the avian malaria parasite, Plasmodium lophurae. J Protozool. 1968 Nov;15(4):763–770. doi: 10.1111/j.1550-7408.1968.tb02209.x. [DOI] [PubMed] [Google Scholar]
  29. Williams G. M., Weisburger E. K., Weisburger J. H. Isolation and long-term cell culture of epithelial-like cells from rat liver. Exp Cell Res. 1971 Nov;69(1):106–112. doi: 10.1016/0014-4827(71)90316-8. [DOI] [PubMed] [Google Scholar]

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