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. 1994 Mar;68(3):2011–2015. doi: 10.1128/jvi.68.3.2011-2015.1994

Intermediates of adeno-associated virus DNA replication in vitro.

G Hong 1, P Ward 1, K I Berns 1
PMCID: PMC236668  PMID: 8107263

Abstract

Intermediates of adeno-associated virus type 2 (AAV) DNA replication in an in vitro assay have been characterized. The assay involves rescue and replication of an AAV insert in pBR322. Intermediates were shown to be duplex molecules in which at least one terminus was in the extended configuration, in contrast to the hairpinned ends seen after rescue in the absence of AAV DNA replication. Also present were linear double-stranded dimers, which were characterized as either head-to-head or tail-to-tail tandems; no head-to-tail dimers were detected. The results are in accord with the current model of AAV DNA replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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