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. 1977;55(5):613–623.

Differentiation of variola, monkeypox, and vaccinia antisera by radioimmunoassay*

Harriet D Hutchinson, Donald W Ziegler, David E Wells, James H Nakano
PMCID: PMC2366687  PMID: 201390

Abstract

Poxvirus antisera adsorbed with “homologous” and “heterologous” poxvirus-infected chorioallantoic membranes (CAM) were differentiated by solid-phase radioimmunoassay (RIA). Mixtures of the antiserum dilutions and infected CAM were added directly (without centrifugation) to poxvirus-infected CAM antigens affixed to wells of microtitration plates. The affixed antigens combined with unadsorbed antibodies, and the cross-reactive antigen—antibody complexes were removed by washing. The results showed that adsorption of an antiserum with variola-, vaccinia-, or uninfected-CAM antigen and subsequent reaction of each in RIA with monkeypox- and uninfected-CAM antigens allowed the identification of antivariola, antivaccinia, or antimonkeypox sera.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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