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. 1977;55(5):569–575.

Preliminary studies on the histochemical differentiation of strains of Onchocerca volvulus microfilariae in Togo*

R A Braun-Munzinger, B A Southgate
PMCID: PMC2366696  PMID: 303958

Abstract

Skin snips were taken from 75 people living in four villages of northern Togo. The 7824 microfilariae that emerged were examined by staining for the presence of acid phosphatase. Four distinct patterns of enzyme staining were observed, and descriptions of the stained microfilariae are given. The study confirms the view that a number of biological strains or variants of Onchocerca volvulus coexist in West Africa, and suggestions are made for further research that could result in the practical application of these observations in onchocerciasis control programmes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J., Fuglsang H., Hamilton P. J., de Marshall T. F. Studies on onchocerciasis in the United Cameroon Republic. II. Comparison of onchocerciasis in rain-forest and Sudan-savanna. Trans R Soc Trop Med Hyg. 1974;68(3):209–222. doi: 10.1016/0035-9203(74)90117-5. [DOI] [PubMed] [Google Scholar]
  2. BUDDEN F. H. Comparative study of ocular onchocerciasis in savannah and rain forest. Trans R Soc Trop Med Hyg. 1963 Jan;57:64–70. doi: 10.1016/0035-9203(63)90013-0. [DOI] [PubMed] [Google Scholar]
  3. Bryceson A. D., van Veen K. S., Oduloju A. J., Duke B. O. Antigenic diversity among Onchocerca volvulus in Nigeria, and immunological differences between onchocerciasis in the savanna and forest of Cameroon. Clin Exp Immunol. 1976 Apr;24(1):168–176. [PMC free article] [PubMed] [Google Scholar]
  4. Chalifoux L. V., Hunt R. D., Garcia F. G., Sehgal P. K., Comiskey J. R. Filariasis in New World monkeys: histochemical differentiation of circulating microfilariae. Lab Anim Sci. 1973 Apr;23(2):211–220. [PubMed] [Google Scholar]
  5. Chalifoux L., Hunt R. D. Histochemical differentiation of Dirofilaria immitis and Dipetalonema reconditum. J Am Vet Med Assoc. 1971 Mar 1;158(5):601–605. [PubMed] [Google Scholar]
  6. Duke B. O., Anderson J. A comparison of the lesions produced in the cornea of the rabbit eye by microfilariae of the forest and Sudan-savanna strains of Onchocerca volvulus from Cameroon. I. The clinical picture. Z Tropenmed Parasitol. 1972 Dec;23(4):354–368. [PubMed] [Google Scholar]
  7. Duke B. O., Lewis D. J., Moore P. J. Onchocerca-Simulium complexes. I. Transmission of forest and Sudan-savanna strains of Onchocerca volvulus, from Cameroon, by Simulium damnosum from various West African bioclimatic zones. Ann Trop Med Parasitol. 1966 Sep;60(3):318–326. [PubMed] [Google Scholar]
  8. Duke B. O., Moore P. J., De León J. R. Onchocerca-simulium complexes. V. The intake and subsequent fate of microfilariae of a Guatemalan strain of Onchocerca volvulus in forest and Sudan-savanna forms of West African Simulium damnosum. Ann Trop Med Parasitol. 1967 Sep;61(3):332–337. [PubMed] [Google Scholar]
  9. Duke B. O. Onchocerca-Simulium complexes. IV. Transmission of a variant of the forest strain on Onchocerca volvulus. Ann Trop Med Parasitol. 1967 Sep;61(3):326–331. [PubMed] [Google Scholar]
  10. Duke B. O. Onchocerca-Simulium complexes. VI. Experimental studies on the transmission of Venezuelan and West African strains of Onchocerca volvulus by Simulium metallicum and S. exiguum in Venezuela. Ann Trop Med Parasitol. 1970 Dec;64(4):421–431. [PubMed] [Google Scholar]
  11. Fuglsang H., Anderson J. The concentration of microfilariae in the skin near the eye as a simple measure of the severity of onchocerciasis in a community and as an indicator of danger to the eye. Tropenmed Parasitol. 1977 Mar;28(1):63–67. [PubMed] [Google Scholar]
  12. Garner A., Duke B. O., Anderson J. A comparison of the lesions produced in the cornea of the rabbit eye by microfilariae of the forest and Sudan-savanna strains of Onchocerca volvulus from Cameroon. II. The pathology. Z Tropenmed Parasitol. 1973 Dec;24(4):385–396. [PubMed] [Google Scholar]
  13. Lewis D. J., Duke B. O. Onchocerca-Simulium complexes. II. Variation in West African female Simulium damnosum. Ann Trop Med Parasitol. 1966 Sep;60(3):337–346. [PubMed] [Google Scholar]
  14. NELSON G. S., GROUNDS J. G. Onchocerciasis at Kodera eleven years after the eradication of the vector. East Afr Med J. 1958 Jul;35(7):365–368. [PubMed] [Google Scholar]
  15. Roberts J. M., Neumann E., Göckel C. W., Highton R. B. Onchocerciasis in Kenya 9, 11 and 18 years after elimination of the vector. Bull World Health Organ. 1967;37(2):195–212. [PMC free article] [PubMed] [Google Scholar]
  16. Romeo De Leon J., Duke B. O. Experimental studies on the transmission of Guatemalan and West African strains of Onchocerca volvulus by Simulium ochraceum, S. metallicum and S. callidum. Trans R Soc Trop Med Hyg. 1966;60(6):735–752. doi: 10.1016/0035-9203(66)90223-9. [DOI] [PubMed] [Google Scholar]
  17. Woodruff A. W., Choyce D. P., Muci-Mendoza F., Hills M., Pettit L. E. Onchocerciasis in Guatemala. A clinical and parasitological study with comparisons between the disease there and in East Africa. Trans R Soc Trop Med Hyg. 1966;60(6):707–719. doi: 10.1016/0035-9203(66)90220-3. [DOI] [PubMed] [Google Scholar]

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