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. 1994;3(3):215–218. doi: 10.1155/S096293519400030X

Serum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel Disease

B Vainerc 1,✉, O H Nielsen 1
PMCID: PMC2367039  PMID: 18472945

Abstract

E-selectin (CD62E) is an endothelial specific glycoprotein belonging to the selectin family of adhesion molecules. Because a high expression of this molecule at intestinal mucosal surfaces in inflammatory bowel disease (IBD) has been described earlier, the aim was to assess serum levels of E-selectin (sE-selectin) and to correlate it to disease activity, and further to evaluate its chemotactic properties at physiological concentrations. Levels of sEselectin were measured by a sandwich ELISA technique in 31 IBD patients together with 15 healthy volunteers. In ulcerative colitis the median value was 0.46 nM (0.16–0.75), in Crohn’s disease 0.47 nM (0.22–1.24), and in healthy controls 0.34 nM (0.22–0.83). No statistically significant differences in sE-selectin were revealed between these groups (p > 0.05). The in vitro chemotactic capabilities of E-selectin (in the concentration range of 0.10–31.4 nM) were assessed using the leading front technique. A significantly increased migratory response was found at concentrations of 1.00 (p < 0.05) and 3.14 nM (p < 0.02). It is concluded that sE-selectin in contrast to sICAM-1 does not act as a sensitive indicator of local immune activation in IBD. However, E-selectin may be important for recruitment and accumulation of neutrophilic granulocytes and other phagocytes involved in the inflammatory process seen in IBD. Future investigations are encouraged in order to reveal its in vivo effects.

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Selected References

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  1. Bevilacqua M. P., Pober J. S., Mendrick D. L., Cotran R. S., Gimbrone M. A., Jr Identification of an inducible endothelial-leukocyte adhesion molecule. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9238–9242. doi: 10.1073/pnas.84.24.9238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bochner B. S., Luscinskas F. W., Gimbrone M. A., Jr, Newman W., Sterbinsky S. A., Derse-Anthony C. P., Klunk D., Schleimer R. P. Adhesion of human basophils, eosinophils, and neutrophils to interleukin 1-activated human vascular endothelial cells: contributions of endothelial cell adhesion molecules. J Exp Med. 1991 Jun 1;173(6):1553–1557. doi: 10.1084/jem.173.6.1553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carlos T., Kovach N., Schwartz B., Rosa M., Newman B., Wayner E., Benjamin C., Osborn L., Lobb R., Harlan J. Human monocytes bind to two cytokine-induced adhesive ligands on cultured human endothelial cells: endothelial-leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1. Blood. 1991 May 15;77(10):2266–2271. [PubMed] [Google Scholar]
  4. Cronstein B. N., Weissmann G. The adhesion molecules of inflammation. Arthritis Rheum. 1993 Feb;36(2):147–157. doi: 10.1002/art.1780360204. [DOI] [PubMed] [Google Scholar]
  5. Damle N. K., Eberhardt C., Van der Vieren M. Direct interaction with primed CD4+ CD45R0+ memory T lymphocytes induces expression of endothelial leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1 on the surface of vascular endothelial cells. Eur J Immunol. 1991 Dec;21(12):2915–2923. doi: 10.1002/eji.1830211204. [DOI] [PubMed] [Google Scholar]
  6. Friedmann P. S., Strickland I., Memon A. A., Johnson P. M. Early time course of recruitment of immune surveillance in human skin after chemical provocation. Clin Exp Immunol. 1993 Mar;91(3):351–356. doi: 10.1111/j.1365-2249.1993.tb05908.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fuggle S. V., Sanderson J. B., Gray D. W., Richardson A., Morris P. J. Variation in expression of endothelial adhesion molecules in pretransplant and transplanted kidneys--correlation with intragraft events. Transplantation. 1993 Jan;55(1):117–123. doi: 10.1097/00007890-199301000-00022. [DOI] [PubMed] [Google Scholar]
  8. Hakkert B. C., Kuijpers T. W., Leeuwenberg J. F., van Mourik J. A., Roos D. Neutrophil and monocyte adherence to and migration across monolayers of cytokine-activated endothelial cells: the contribution of CD18, ELAM-1, and VLA-4. Blood. 1991 Nov 15;78(10):2721–2726. [PubMed] [Google Scholar]
  9. Koizumi M., King N., Lobb R., Benjamin C., Podolsky D. K. Expression of vascular adhesion molecules in inflammatory bowel disease. Gastroenterology. 1992 Sep;103(3):840–847. doi: 10.1016/0016-5085(92)90015-q. [DOI] [PubMed] [Google Scholar]
  10. Krugluger W., Lill W., Nell A., Katzensteiner S., Sperr W., Förster O. Lectin binding to chronic inflammatory gingival tissue: possible adhesion mechanisms based on lectin-carbohydrate interactions. J Periodontal Res. 1993 Mar;28(2):145–151. doi: 10.1111/j.1600-0765.1993.tb01062.x. [DOI] [PubMed] [Google Scholar]
  11. Langholz E., Munkholm P., Nielsen O. H., Kreiner S., Binder V. Incidence and prevalence of ulcerative colitis in Copenhagen county from 1962 to 1987. Scand J Gastroenterol. 1991 Dec;26(12):1247–1256. doi: 10.3109/00365529108998621. [DOI] [PubMed] [Google Scholar]
  12. Leeuwenberg J. F., Jeunhomme T. M., Buurman W. A. Role of ELAM-1 in adhesion of monocytes to activated human endothelial cells. Scand J Immunol. 1992 Mar;35(3):335–341. doi: 10.1111/j.1365-3083.1992.tb02866.x. [DOI] [PubMed] [Google Scholar]
  13. Lo S. K., Lee S., Ramos R. A., Lobb R., Rosa M., Chi-Rosso G., Wright S. D. Endothelial-leukocyte adhesion molecule 1 stimulates the adhesive activity of leukocyte integrin CR3 (CD11b/CD18, Mac-1, alpha m beta 2) on human neutrophils. J Exp Med. 1991 Jun 1;173(6):1493–1500. doi: 10.1084/jem.173.6.1493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Luscinskas F. W., Brock A. F., Arnaout M. A., Gimbrone M. A., Jr Endothelial-leukocyte adhesion molecule-1-dependent and leukocyte (CD11/CD18)-dependent mechanisms contribute to polymorphonuclear leukocyte adhesion to cytokine-activated human vascular endothelium. J Immunol. 1989 Apr 1;142(7):2257–2263. [PubMed] [Google Scholar]
  15. Matis W. L., Lavker R. M., Murphy G. F. Substance P induces the expression of an endothelial-leukocyte adhesion molecule by microvascular endothelium. J Invest Dermatol. 1990 Apr;94(4):492–495. doi: 10.1111/1523-1747.ep12874665. [DOI] [PubMed] [Google Scholar]
  16. Munkholm P., Langholz E., Nielsen O. H., Kreiner S., Binder V. Incidence and prevalence of Crohn's disease in the county of Copenhagen, 1962-87: a sixfold increase in incidence. Scand J Gastroenterol. 1992 Jul;27(7):609–614. doi: 10.3109/00365529209000127. [DOI] [PubMed] [Google Scholar]
  17. Nielsen O. H., Elmgreen J. Activation of neutrophil chemotaxis by leukotriene B4 and 5-hydroxyeicosatetraenoic acid in chronic inflammatory bowel disease. Scand J Clin Lab Invest. 1987 Oct;47(6):605–611. doi: 10.1080/00365518709168476. [DOI] [PubMed] [Google Scholar]
  18. Ohtani H., Nakamura S., Watanabe Y., Fukushima K., Mizoi T., Kimura M., Hiwatashi N., Nagura H. Light and electron microscopic immunolocalization of endothelial leucocyte adhesion molecule-1 in inflammatory bowel disease. Morphological evidence of active synthesis and secretion into vascular lumen. Virchows Arch A Pathol Anat Histopathol. 1992;420(5):403–409. doi: 10.1007/BF01600511. [DOI] [PubMed] [Google Scholar]
  19. Shimizu Y., Newman W., Gopal T. V., Horgan K. J., Graber N., Beall L. D., van Seventer G. A., Shaw S. Four molecular pathways of T cell adhesion to endothelial cells: roles of LFA-1, VCAM-1, and ELAM-1 and changes in pathway hierarchy under different activation conditions. J Cell Biol. 1991 Jun;113(5):1203–1212. doi: 10.1083/jcb.113.5.1203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Steinhoff G., Behrend M., Schrader B., Duijvestijn A. M., Wonigeit K. Expression patterns of leukocyte adhesion ligand molecules on human liver endothelia. Lack of ELAM-1 and CD62 inducibility on sinusoidal endothelia and distinct distribution of VCAM-1, ICAM-1, ICAM-2, and LFA-3. Am J Pathol. 1993 Feb;142(2):481–488. [PMC free article] [PubMed] [Google Scholar]
  21. van der Wal A. C., Das P. K., Tigges A. J., Becker A. E. Adhesion molecules on the endothelium and mononuclear cells in human atherosclerotic lesions. Am J Pathol. 1992 Dec;141(6):1427–1433. [PMC free article] [PubMed] [Google Scholar]

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