Abstract
Positively charged macromolecules cause a variety of pathological events through their electrostatic interaction with anionic sites present on the membrane of target cells. In the present study we have investigated the effect of hyaluronic acid, a negatively charged molecule, on rat paw oedema induced by poly-L-lysine as well as on histamine release from rat mast cells and nitric oxide formation from rabbit aorta, both induced by this polycation. The results indicate that hyaluronic acid is able to suppress these poly-L-lysine induced effects with a mechanism which possibly depends on its negative charges which may balance the effects of positively charged polycations.
Full Text
The Full Text of this article is available as a PDF (281.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Antunes E., Mariano M., Cirino G., Levi S., de Nucci G. Pharmacological characterization of polycation-induced rat hind-paw oedema. Br J Pharmacol. 1990 Dec;101(4):986–990. doi: 10.1111/j.1476-5381.1990.tb14193.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atkinson G., Ennis M., Pearce F. L. The effect of alkaline earth cations on the release of histamine from rat peritoneal mast cells treated with compound 48/80 and peptide 401. Br J Pharmacol. 1979 Mar;65(3):395–402. doi: 10.1111/j.1476-5381.1979.tb07843.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Di Rosa M., Willoughby D. A. Screens for anti-inflammatory drugs. J Pharm Pharmacol. 1971 Apr;23(4):297–298. doi: 10.1111/j.2042-7158.1971.tb08661.x. [DOI] [PubMed] [Google Scholar]
- Foreman J. C., Lichtenstein L. M. Induction of histamine secretion by polycations. Biochim Biophys Acta. 1980 May 22;629(3):587–603. doi: 10.1016/0304-4165(80)90164-6. [DOI] [PubMed] [Google Scholar]
- Hecker M., Siegle I., Macarthur H., Sessa W. C., Vane J. R. Role of intracellular thiols in release of EDRF from cultured endothelial cells. Am J Physiol. 1992 Mar;262(3 Pt 2):H888–H896. doi: 10.1152/ajpheart.1992.262.3.H888. [DOI] [PubMed] [Google Scholar]
- Ialenti A., Ianaro A., Moncada S., Di Rosa M. Modulation of acute inflammation by endogenous nitric oxide. Eur J Pharmacol. 1992 Feb 11;211(2):177–182. doi: 10.1016/0014-2999(92)90526-a. [DOI] [PubMed] [Google Scholar]
- Moncada S., Palmer R. M., Higgs E. A. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev. 1991 Jun;43(2):109–142. [PubMed] [Google Scholar]
- Needham L., Hellewell P. G., Williams T. J., Gordon J. L. Endothelial functional responses and increased vascular permeability induced by polycations. Lab Invest. 1988 Oct;59(4):538–548. [PubMed] [Google Scholar]
- SHORE P. A., BURKHALTER A., COHN V. H., Jr A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther. 1959 Nov;127:182–186. [PubMed] [Google Scholar]
- Sato H., Takahashi T., Ide H., Fukushima T., Tabata M., Sekine F., Kobayashi K., Negishi M., Niwa Y. Antioxidant activity of synovial fluid, hyaluronic acid, and two subcomponents of hyaluronic acid. Synovial fluid scavenging effect is enhanced in rheumatoid arthritis patients. Arthritis Rheum. 1988 Jan;31(1):63–71. doi: 10.1002/art.1780310110. [DOI] [PubMed] [Google Scholar]
- Skutelsky E., Danon D. Redistribution of surface anionic sites on the luminal front of blood vessel endothelium after interaction with polycationic ligand. J Cell Biol. 1976 Oct;71(1):232–241. doi: 10.1083/jcb.71.1.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Skutelsky E., Rudich Z., Danon D. Surface charge properties of the luminal front of blood vessel walls: an electron microscopical analysis. Thromb Res. 1975 Oct;7(4):623–634. doi: 10.1016/0049-3848(75)90108-5. [DOI] [PubMed] [Google Scholar]
- Thomas G., Hecker M., Ramwell P. W. Vascular activity of polycations and basic amino acids: L-arginine does not specifically elicit endothelium-dependent relaxation. Biochem Biophys Res Commun. 1989 Jan 16;158(1):177–180. doi: 10.1016/s0006-291x(89)80194-9. [DOI] [PubMed] [Google Scholar]