Abstract
We have analyzed atypical tomato bushy stunt virus defective interfering (DI) RNA species which accumulated during a passage series in protoplasts. We present a rationale for the order of appearance of these molecules and show, using competition assays, that either segment duplication or single nucleotide insertion can enhance DI RNA competitiveness. Possible mechanisms for the introduction of the modifications observed in these DI RNAs are discussed.
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Selected References
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- Carpenter C. D., Cascone P. J., Simon A. E. Mutations in a satellite RNA of turnip crinkle virus result in addition of poly(U) in vivo. Virology. 1991 Aug;183(2):595–601. doi: 10.1016/0042-6822(91)90988-n. [DOI] [PubMed] [Google Scholar]
- Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drake J. W. Rates of spontaneous mutation among RNA viruses. Proc Natl Acad Sci U S A. 1993 May 1;90(9):4171–4175. doi: 10.1073/pnas.90.9.4171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finnen R. L., Rochon D. M. Sequence and structure of defective interfering RNAs associated with cucumber necrosis virus infections. J Gen Virol. 1993 Aug;74(Pt 8):1715–1720. doi: 10.1099/0022-1317-74-8-1715. [DOI] [PubMed] [Google Scholar]
- Hearne P. Q., Knorr D. A., Hillman B. I., Morris T. J. The complete genome structure and synthesis of infectious RNA from clones of tomato bushy stunt virus. Virology. 1990 Jul;177(1):141–151. doi: 10.1016/0042-6822(90)90468-7. [DOI] [PubMed] [Google Scholar]
- Hillman B. I., Carrington J. C., Morris T. J. A defective interfering RNA that contains a mosaic of a plant virus genome. Cell. 1987 Nov 6;51(3):427–433. doi: 10.1016/0092-8674(87)90638-6. [DOI] [PubMed] [Google Scholar]
- Jalanko A., Söderlund H. The repeated regions of Semliki Forest virus defective-inferfering RNA interferes with the encapsidation process of the standard virus. Virology. 1985 Mar;141(2):257–266. doi: 10.1016/0042-6822(85)90256-9. [DOI] [PubMed] [Google Scholar]
- Knorr D. A., Mullin R. H., Hearne P. Q., Morris T. J. De novo generation of defective interfering RNAs of tomato bushy stunt virus by high multiplicity passage. Virology. 1991 Mar;181(1):193–202. doi: 10.1016/0042-6822(91)90484-S. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lazzarini R. A., Keene J. D., Schubert M. The origins of defective interfering particles of the negative-strand RNA viruses. Cell. 1981 Oct;26(2 Pt 2):145–154. doi: 10.1016/0092-8674(81)90298-1. [DOI] [PubMed] [Google Scholar]
- Li X. H., Heaton L. A., Morris T. J., Simon A. E. Turnip crinkle virus defective interfering RNAs intensify viral symptoms and are generated de novo. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9173–9177. doi: 10.1073/pnas.86.23.9173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li X. H., Simon A. E. In vivo accumulation of a turnip crinkle virus defective interfering RNA is affected by alterations in size and sequence. J Virol. 1991 Sep;65(9):4582–4590. doi: 10.1128/jvi.65.9.4582-4590.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li Y., Ball L. A. Nonhomologous RNA recombination during negative-strand synthesis of flock house virus RNA. J Virol. 1993 Jul;67(7):3854–3860. doi: 10.1128/jvi.67.7.3854-3860.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Makino S., Shieh C. K., Soe L. H., Baker S. C., Lai M. M. Primary structure and translation of a defective interfering RNA of murine coronavirus. Virology. 1988 Oct;166(2):550–560. doi: 10.1016/0042-6822(88)90526-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nagy P. D., Bujarski J. J. Targeting the site of RNA-RNA recombination in brome mosaic virus with antisense sequences. Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6390–6394. doi: 10.1073/pnas.90.14.6390. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perrault J. Origin and replication of defective interfering particles. Curr Top Microbiol Immunol. 1981;93:151–207. doi: 10.1007/978-3-642-68123-3_7. [DOI] [PubMed] [Google Scholar]
- Rochon D. M., Johnston J. C. Infectious transcripts from cloned cucumber necrosis virus cDNA: evidence for a bifunctional subgenomic mRNA. Virology. 1991 Apr;181(2):656–665. doi: 10.1016/0042-6822(91)90899-m. [DOI] [PubMed] [Google Scholar]
- Rochon D. M. Rapid de novo generation of defective interfering RNA by cucumber necrosis virus mutants that do not express the 20-kDa nonstructural protein. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11153–11157. doi: 10.1073/pnas.88.24.11153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rochon D. M., Tremaine J. H. Complete nucleotide sequence of the cucumber necrosis virus genome. Virology. 1989 Apr;169(2):251–259. doi: 10.1016/0042-6822(89)90150-5. [DOI] [PubMed] [Google Scholar]
- Romero J., Huang Q., Pogany J., Bujarski J. J. Characterization of defective interfering RNA components that increase symptom severity of broad bean mottle virus infections. Virology. 1993 Jun;194(2):576–584. doi: 10.1006/viro.1993.1297. [DOI] [PubMed] [Google Scholar]
- Roux L., Simon A. E., Holland J. J. Effects of defective interfering viruses on virus replication and pathogenesis in vitro and in vivo. Adv Virus Res. 1991;40:181–211. doi: 10.1016/S0065-3527(08)60279-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White K. A., Bancroft J. B., Mackie G. A. Defective RNAs of clover yellow mosaic virus encode nonstructural/coat protein fusion products. Virology. 1991 Aug;183(2):479–486. doi: 10.1016/0042-6822(91)90977-J. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White K. A., Morris T. J. Nonhomologous RNA recombination in tombusviruses: generation and evolution of defective interfering RNAs by stepwise deletions. J Virol. 1994 Jan;68(1):14–24. doi: 10.1128/jvi.68.1.14-24.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White K. A., Morris T. J. Recombination between defective tombusvirus RNAs generates functional hybrid genomes. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3642–3646. doi: 10.1073/pnas.91.9.3642. [DOI] [PMC free article] [PubMed] [Google Scholar]