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. 1994 Sep;68(9):6116–6119. doi: 10.1128/jvi.68.9.6116-6119.1994

Identification of the putative RNA polymerase of Cryphonectria hypovirus in a solubilized replication complex.

T Fahima 1, Y Wu 1, L Zhang 1, N K Van Alfen 1
PMCID: PMC237024  PMID: 8057493

Abstract

Hypovirulence of the pathogenic fungus Cryphonectria parasitica, caused by the unencapsidated viral double-stranded RNA of Cryphonectria hypovirus (CHV1), provides a means for biological control of chestnut blight. We report here the isolation of a replication complex of the virus solubilized from host membranes. The conserved regions of the putative RNA polymerase encoded by strain CHV1-713 were cloned and expressed, and the recombinant protein was purified and used to produce polyclonal antibodies. The CHV1 replication complex was solubilized from a membrane fraction of CHV1-infected C. parasitica hyphae. Antibodies raised against the putative viral polymerase reacted on a Western immunoblot with an 87-kDa polypeptide of the replication complex but not with comparable preparations from an isogenic uninfected strain. Analysis of the polypeptide composition of the complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining revealed a number of other polypeptides along with the double-stranded RNA of the virus. We conclude that this 87-kDa polypeptide is the putative RNA polymerase encoded on open reading frame B of CHV1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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