Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1994 Dec;68(12):7738–7745. doi: 10.1128/jvi.68.12.7738-7745.1994

Mutational analysis of the capsid protein of Leishmania RNA virus LRV1-4.

T L Cadd 1, K MacBeth 1, D Furlong 1, J L Patterson 1
PMCID: PMC237235  PMID: 7966563

Abstract

The virion of Leishmania RNA virus is predicted to be composed of a 742-amino-acid major capsid protein and a small percentage of capsid-polymerase fusion molecules. Recently, the capsid protein alone was expressed and shown to spontaneously assemble into viruslike particles. Since the major structural protein of the virion shell self-assembles into viruslike particles when expressed in the baculovirus expression system, assembly of the virion can be studied by mutational analysis and expression of a single open reading frame. In this study, several deletions and one addition of the capsid protein of Leishmania RNA virus LRV1-4 were generated. These mutants show different degrees of assembly. Assembly domains are being identified such that the capsid protein may be used as a macromolecular packaging and delivery system for Leishmania species.

Full text

PDF
7738

Images in this article

7741Image
on p.7741
7742Image
on p.7742
7744Image
on p.7744

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong T. C., Keenan M. C., Widmer G., Patterson J. L. Successful transient introduction of Leishmania RNA virus into a virally infected and an uninfected strain of Leishmania. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1736–1740. doi: 10.1073/pnas.90.5.1736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bellofatto V., Cross G. A. Expression of a bacterial gene in a trypanosomatid protozoan. Science. 1989 Jun 9;244(4909):1167–1169. doi: 10.1126/science.2499047. [DOI] [PubMed] [Google Scholar]
  3. Bruenn J. A. Relationships among the positive strand and double-strand RNA viruses as viewed through their RNA-dependent RNA polymerases. Nucleic Acids Res. 1991 Jan 25;19(2):217–226. doi: 10.1093/nar/19.2.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cadd T. L., Keenan M. C., Patterson J. L. Detection of Leishmania RNA virus 1 proteins. J Virol. 1993 Sep;67(9):5647–5650. doi: 10.1128/jvi.67.9.5647-5650.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cadd T. L., Patterson J. L. Synthesis of viruslike particles by expression of the putative capsid protein of Leishmania RNA virus in a recombinant baculovirus expression system. J Virol. 1994 Jan;68(1):358–365. doi: 10.1128/jvi.68.1.358-365.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chung I. K., Armstrong T. C., Patterson J. L. Identification of a short viral transcript in Leishmania RNA virus-infected cells. Virology. 1994 Feb;198(2):552–556. doi: 10.1006/viro.1994.1066. [DOI] [PubMed] [Google Scholar]
  7. Dinman J. D., Icho T., Wickner R. B. A -1 ribosomal frameshift in a double-stranded RNA virus of yeast forms a gag-pol fusion protein. Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):174–178. doi: 10.1073/pnas.88.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. French T. J., Roy P. Synthesis of bluetongue virus (BTV) corelike particles by a recombinant baculovirus expressing the two major structural core proteins of BTV. J Virol. 1990 Apr;64(4):1530–1536. doi: 10.1128/jvi.64.4.1530-1536.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fujimura T., Wickner R. B. Gene overlap results in a viral protein having an RNA binding domain and a major coat protein domain. Cell. 1988 Nov 18;55(4):663–671. doi: 10.1016/0092-8674(88)90225-5. [DOI] [PubMed] [Google Scholar]
  10. Hatton T., Zhou S., Standring D. N. RNA- and DNA-binding activities in hepatitis B virus capsid protein: a model for their roles in viral replication. J Virol. 1992 Sep;66(9):5232–5241. doi: 10.1128/jvi.66.9.5232-5241.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Icho T., Wickner R. B. The double-stranded RNA genome of yeast virus L-A encodes its own putative RNA polymerase by fusing two open reading frames. J Biol Chem. 1989 Apr 25;264(12):6716–6723. [PubMed] [Google Scholar]
  12. Jiang X., Wang M., Graham D. Y., Estes M. K. Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein. J Virol. 1992 Nov;66(11):6527–6532. doi: 10.1128/jvi.66.11.6527-6532.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laban A., Wirth D. F. Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9119–9123. doi: 10.1073/pnas.86.23.9119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ruffing M., Zentgraf H., Kleinschmidt J. A. Assembly of viruslike particles by recombinant structural proteins of adeno-associated virus type 2 in insect cells. J Virol. 1992 Dec;66(12):6922–6930. doi: 10.1128/jvi.66.12.6922-6930.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Scheffter S., Widmer G., Patterson J. L. Complete sequence of Leishmania RNA virus 1-4 and identification of conserved sequences. Virology. 1994 Mar;199(2):479–483. doi: 10.1006/viro.1994.1149. [DOI] [PubMed] [Google Scholar]
  16. Stuart K. D., Weeks R., Guilbride L., Myler P. J. Molecular organization of Leishmania RNA virus 1. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8596–8600. doi: 10.1073/pnas.89.18.8596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tarr P. I., Aline R. F., Jr, Smiley B. L., Scholler J., Keithly J., Stuart K. LR1: a candidate RNA virus of Leishmania. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9572–9575. doi: 10.1073/pnas.85.24.9572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Valentine R. C., Green N. M. Electron microscopy of an antibody-hapten complex. J Mol Biol. 1967 Aug 14;27(3):615–617. doi: 10.1016/0022-2836(67)90063-0. [DOI] [PubMed] [Google Scholar]
  19. Wang A. L., Yang H. M., Shen K. A., Wang C. C. Giardiavirus double-stranded RNA genome encodes a capsid polypeptide and a gag-pol-like fusion protein by a translation frameshift. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8595–8599. doi: 10.1073/pnas.90.18.8595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weeks R. S., Patterson J. L., Stuart K., Widmer G. Transcribing and replicating particles in a double-stranded RNA virus from Leishmania. Mol Biochem Parasitol. 1992 Jun;52(2):207–213. doi: 10.1016/0166-6851(92)90053-m. [DOI] [PubMed] [Google Scholar]
  21. Widmer G., Comeau A. M., Furlong D. B., Wirth D. F., Patterson J. L. Characterization of a RNA virus from the parasite Leishmania. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5979–5982. doi: 10.1073/pnas.86.15.5979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES