Abstract
The agent(s) responsible for sporadic non-A, non-B hepatitis in humans was serially transmitted in rhesus monkeys by intravenous inoculation of the stool extract from a patient. A novel agent called HFV (hepatitis French [origin] virus) was present as 27- to 37-nm particles in the infectious stool extract. Hepatopathic lesions were noticed in infected monkeys during the acute phase of illness. The purified viral 27- to 37-nm particles consist of a double-stranded DNA of approximately 20 kb and are detected in infected monkey liver. Analysis of cell culture detects the approximately 20-kb-long viral DNA in stool samples from infected monkeys and sporadic enteric non-A, non-B hepatitis patients. Furthermore, the 27- to 37-nm viral particles were able to protect monkeys challenged with infectious stool extract. Our results indicate that 27- to 37-nm virus like particles are responsible for sporadic non-A, non-B hepatitis in rhesus monkeys.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Acheson D. W., Leen C. L., Tariq W. U., Mandal B. K. Non-parenterally transmitted non-A, non-B hepatitis in children in the UK. Lancet. 1987 Oct 3;2(8562):798–799. doi: 10.1016/s0140-6736(87)92529-3. [DOI] [PubMed] [Google Scholar]
- Bradley D. W., Krawczynski K., Cook E. H., Jr, McCaustland K. A., Humphrey C. D., Spelbring J. E., Myint H., Maynard J. E. Enterically transmitted non-A, non-B hepatitis: serial passage of disease in cynomolgus macaques and tamarins and recovery of disease-associated 27- to 34-nm viruslike particles. Proc Natl Acad Sci U S A. 1987 Sep;84(17):6277–6281. doi: 10.1073/pnas.84.17.6277. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Caredda F., Antinori S., Pastecchia C., Coppin P., Moroni M. Sporadic non-A, non-B hepatitis. Lancet. 1988 Jan 2;1(8575-6):58–58. doi: 10.1016/s0140-6736(88)91038-0. [DOI] [PubMed] [Google Scholar]
- Francis D. P., Hadler S. C., Prendergast T. J., Peterson E., Ginsberg M. M., Lookabaugh C., Holmes J. R., Maynard J. E. Occurrence of hepatitis A, B, and non-A/non-B in the United States. CDC sentinel county hepatitis study I. Am J Med. 1984 Jan;76(1):69–74. doi: 10.1016/0002-9343(84)90752-6. [DOI] [PubMed] [Google Scholar]
- Khuroo M. S., Duermeyer W., Zargar S. A., Ahanger M. A., Shah M. A. Acute sporadic non-A, non-B hepatitis in India. Am J Epidemiol. 1983 Sep;118(3):360–364. doi: 10.1093/oxfordjournals.aje.a113643. [DOI] [PubMed] [Google Scholar]
- Krawczynski K. Hepatitis E. Hepatology. 1993 May;17(5):932–941. [PubMed] [Google Scholar]
- Panda S. K., Datta R., Kaur J., Zuckerman A. J., Nayak N. C. Enterically transmitted non-A, non-B hepatitis: recovery of virus-like particles from an epidemic in south Delhi and transmission studies in rhesus monkeys. Hepatology. 1989 Oct;10(4):466–472. doi: 10.1002/hep.1840100411. [DOI] [PubMed] [Google Scholar]
- Reyes G. R., Purdy M. A., Kim J. P., Luk K. C., Young L. M., Fry K. E., Bradley D. W. Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science. 1990 Mar 16;247(4948):1335–1339. doi: 10.1126/science.2107574. [DOI] [PubMed] [Google Scholar]
- Seelig R., Liehr H., Seelig H. P. Detection of a non-A, non-B hepatitis associated substance in stools. Dev Biol Stand. 1983;54:497–500. [PubMed] [Google Scholar]
- Sharma M. D., Maillard P., Vrati S., Mukherjee R., Rajagopalan M. S., Poynard T., Talwar G. P., Pillot J. Serial passage of west-European sporadic non-A non-B hepatitis in rhesus monkeys by inoculation with fecal extracts. J Med Virol. 1990 Jan;30(1):36–41. doi: 10.1002/jmv.1890300108. [DOI] [PubMed] [Google Scholar]
- Talwar G. P., Banerjee K., Reddi P. P., Sharma M., Qadri A., Gupta S. K., Mukherjee R., Ray R., Ghosh S., Deka N. Diagnostics for the tropical countries. J Immunol Methods. 1992 Jun 24;150(1-2):121–132. doi: 10.1016/0022-1759(92)90071-z. [DOI] [PubMed] [Google Scholar]