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. 1994 Dec;68(12):7840–7849. doi: 10.1128/jvi.68.12.7840-7849.1994

Chinese hamster ovary cells contain transcriptionally active full-length type C proviruses.

Y S Lie 1, E M Penuel 1, M A Low 1, T P Nguyen 1, J O Mangahas 1, K P Anderson 1, C J Petropoulos 1
PMCID: PMC237246  PMID: 7966574

Abstract

We have isolated a genomic locus from Chinese hamster ovary (CHO) cells that contains a full-length provirus. Nucleotide sequence analysis indicates that it is a defective member of the rodent type C retrovirus family with an env region that is similar to those of mouse amphotropic retrovirus and subgroup B feline leukemia virus. We were able to demonstrate that this provirus is a member of a closely related family of full-length proviruses in CHO cells and Chinese hamster liver. Hybridization probes generated from this genomic clone were used to characterize type C retrovirus RNA expression in CHO cells. Full-length genomic RNA and subgenomic envelope mRNA were detected in CHO cell lines but not in the human-derived 293 cell line. Interestingly, we discovered that the site of retrovirus integration lies within a G repeat sequence belonging to the short interspersed element family of retroposons.

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Selected References

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