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. 1993 Jan;67(1):606–609. doi: 10.1128/jvi.67.1.606-609.1993

Marker rescue of a transformation-negative Epstein-Barr virus recombinant from an infected Burkitt lymphoma cell line: a method useful for analysis of genes essential for transformation.

A Marchini 1, E Kieff 1, R Longnecker 1
PMCID: PMC237404  PMID: 8380096

Abstract

A Burkitt lymphoma cell line infected in vitro with a transformation-defective mutant recombinant Epstein-Barr virus (EBV) was used to attempt marker rescue of transformation competence by transfection with cloned wild-type DNA. EBV replication was induced in the transfected cells, and wild-type EBV DNA recombined via flanking homologous sequences adjacent to the deletion, resulting in a virus which transformed primary B lymphocytes in vitro. This strategy should be useful for molecular genetic analysis of the role of part or all of any gene in cell growth transformation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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