Abstract
Patterns of allele-specific expression of H19 and insulin-like growth factor-2 (IGF-2) were examined in tissue obtained from 30 children diagnosed with hepatoblastoma. All informative tumours demonstrated monoallelic expression of H19. In contrast, variable patterns of allele-specific expression of IGF-2 were seen in tumours from children of different ages.© 2000 Cancer Research Campaign
Keywords: IGF-2, H19, hepatoblastoma, genomic imprinting
Full Text
The Full Text of this article is available as a PDF (90.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Davies S. M. Developmental regulation of genomic imprinting of the IGF2 gene in human liver. Cancer Res. 1994 May 15;54(10):2560–2562. [PubMed] [Google Scholar]
- Davies S. M. Maintenance of genomic imprinting at the IGF2 locus in hepatoblastoma. Cancer Res. 1993 Oct 15;53(20):4781–4783. [PubMed] [Google Scholar]
- Ekström T. J., Cui H., Li X., Ohlsson R. Promoter-specific IGF2 imprinting status and its plasticity during human liver development. Development. 1995 Feb;121(2):309–316. doi: 10.1242/dev.121.2.309. [DOI] [PubMed] [Google Scholar]
- Glassman M. L., de Groot N., Hochberg A. Relaxation of imprinting in carcinogenesis. Cancer Genet Cytogenet. 1996 Jul 1;89(1):69–73. doi: 10.1016/0165-4608(95)00364-9. [DOI] [PubMed] [Google Scholar]
- Hu J. F., Vu T. H., Hoffman A. R. Promoter-specific modulation of insulin-like growth factor II genomic imprinting by inhibitors of DNA methylation. J Biol Chem. 1996 Jul 26;271(30):18253–18262. doi: 10.1074/jbc.271.30.18253. [DOI] [PubMed] [Google Scholar]
- Leisenring W. M., Breslow N. E., Evans I. E., Beckwith J. B., Coppes M. J., Grundy P. Increased birth weights of National Wilms' Tumor Study patients suggest a growth factor excess. Cancer Res. 1994 Sep 1;54(17):4680–4683. [PubMed] [Google Scholar]
- Li X., Adam G., Cui H., Sandstedt B., Ohlsson R., Ekström T. J. Expression, promoter usage and parental imprinting status of insulin-like growth factor II (IGF2) in human hepatoblastoma: uncoupling of IGF2 and H19 imprinting. Oncogene. 1995 Jul 20;11(2):221–229. [PubMed] [Google Scholar]
- Li X., Cui H., Sandstedt B., Nordlinder H., Larsson E., Ekström T. J. Expression levels of the insulin-like growth factor-II gene (IGF2) in the human liver: developmental relationships of the four promoters. J Endocrinol. 1996 Apr;149(1):117–124. doi: 10.1677/joe.0.1490117. [DOI] [PubMed] [Google Scholar]
- Li X., Gray S. G., Flam F., Pietsch T., Ekström T. J. Developmental-dependent DNA methylation of the IGF2 and H19 promoters is correlated to the promoter activities in human liver development. Int J Dev Biol. 1998 Jul;42(5):687–693. [PubMed] [Google Scholar]
- Li X., Nong Z., Ekström C., Larsson E., Nordlinder H., Hofmann W. J., Trautwein C., Odenthal M., Dienes H. P., Ekström T. J. Disrupted IGF2 promoter control by silencing of promoter P1 in human hepatocellular carcinoma. Cancer Res. 1997 May 15;57(10):2048–2054. [PubMed] [Google Scholar]
- Nonomura N., Nishimura K., Miki T., Kanno N., Kojima Y., Yokoyama M., Okuyama A. Loss of imprinting of the insulin-like growth factor II gene in renal cell carcinoma. Cancer Res. 1997 Jul 1;57(13):2575–2577. [PubMed] [Google Scholar]
- Ogawa O., Eccles M. R., Szeto J., McNoe L. A., Yun K., Maw M. A., Smith P. J., Reeve A. E. Relaxation of insulin-like growth factor II gene imprinting implicated in Wilms' tumour. Nature. 1993 Apr 22;362(6422):749–751. doi: 10.1038/362749a0. [DOI] [PubMed] [Google Scholar]
- Ohlsson R., Nyström A., Pfeifer-Ohlsson S., Töhönen V., Hedborg F., Schofield P., Flam F., Ekström T. J. IGF2 is parentally imprinted during human embryogenesis and in the Beckwith-Wiedemann syndrome. Nat Genet. 1993 May;4(1):94–97. doi: 10.1038/ng0593-94. [DOI] [PubMed] [Google Scholar]
- Rainier S., Dobry C. J., Feinberg A. P. Loss of imprinting in hepatoblastoma. Cancer Res. 1995 May 1;55(9):1836–1838. [PubMed] [Google Scholar]
- Rainier S., Johnson L. A., Dobry C. J., Ping A. J., Grundy P. E., Feinberg A. P. Relaxation of imprinted genes in human cancer. Nature. 1993 Apr 22;362(6422):747–749. doi: 10.1038/362747a0. [DOI] [PubMed] [Google Scholar]
- Ross J. A., Schmidt P. T., Perentesis J. P., Davies S. M. Genomic imprinting of H19 and insulin-like growth factor-2 in pediatric germ cell tumors. Cancer. 1999 Mar 15;85(6):1389–1394. [PubMed] [Google Scholar]
- Takeda S., Kondo M., Kumada T., Koshikawa T., Ueda R., Nishio M., Osada H., Suzuki H., Nagatake M., Washimi O. Allelic-expression imbalance of the insulin-like growth factor 2 gene in hepatocellular carcinoma and underlying disease. Oncogene. 1996 Apr 4;12(7):1589–1592. [PubMed] [Google Scholar]
- Taniguchi T., Schofield A. E., Scarlett J. L., Morison I. M., Sullivan M. J., Reeve A. E. Altered specificity of IGF2 promoter imprinting during fetal development and onset of Wilms tumour. Oncogene. 1995 Aug 17;11(4):751–756. [PubMed] [Google Scholar]
- Uyeno S., Aoki Y., Nata M., Sagisaka K., Kayama T., Yoshimoto T., Ono T. IGF2 but not H19 shows loss of imprinting in human glioma. Cancer Res. 1996 Dec 1;56(23):5356–5359. [PubMed] [Google Scholar]
- Vu T. H., Hoffman A. Alterations in the promoter-specific imprinting of the insulin-like growth factor-II gene in Wilms' tumor. J Biol Chem. 1996 Apr 12;271(15):9014–9023. doi: 10.1074/jbc.271.15.9014. [DOI] [PubMed] [Google Scholar]
- Zemel S., Bartolomei M. S., Tilghman S. M. Physical linkage of two mammalian imprinted genes, H19 and insulin-like growth factor 2. Nat Genet. 1992 Sep;2(1):61–65. doi: 10.1038/ng0992-61. [DOI] [PubMed] [Google Scholar]
- van Gurp R. J., Oosterhuis J. W., Kalscheuer V., Mariman E. C., Looijenga L. H. Biallelic expression of the H19 and IGF2 genes in human testicular germ cell tumors. J Natl Cancer Inst. 1994 Jul 20;86(14):1070–1075. doi: 10.1093/jnci/86.14.1070. [DOI] [PubMed] [Google Scholar]