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. 2000 Feb 1;82(5):1000–1003. doi: 10.1054/bjoc.1999.1032

Tumour necrosis factor alpha increases melphalan concentration in tumour tissue after isolated limb perfusion

J H W de Wilt 1, T L M ten Hagen 1, G de Boeck 2, S T van Tiel 1, E A de Bruijn 3, A M M Eggermont 1
PMCID: PMC2374420  PMID: 10737379

Abstract

Several possible mechanisms for the synergistic anti-tumour effects between tumour necrosis factor alpha (TNF-α) and melphalan after isolated limb perfusion (ILP) have been presented. We found a significant sixfold increase in melphalan tumour tissue concentration after ILP when TNF-α was added to the perfusate, which provides a straightforward explanation for the observed synergism between melphalan and TNF-α in ILP. © 2000 Cancer Research Campaign

Keywords: TNF, melphalan, tissue concentration, isolated limb perfusion, rats

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