Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1993 Feb;67(2):1122–1126. doi: 10.1128/jvi.67.2.1122-1126.1993

Sequence variation of human endogenous retrovirus ERV9-related elements in an env region corresponding to an immunosuppressive peptide: transcription in normal and neoplastic cells.

M Lindeskog 1, P Medstrand 1, J Blomberg 1
PMCID: PMC237471  PMID: 8419641

Abstract

Evolutionarily conserved sequences corresponding to an immunosuppressive region in retroviral transmembrane proteins were amplified by the polymerase chain reaction from human genomic DNA and reverse-transcribed RNA from one glioma, three pieces of macroscopically normal brain tissue, kidney, lymphocytes, cultured embryonic lung cells, and a rhabdomyosarcoma cell line. Amplification products (125 bp) from DNA and RNA from the glioma and RNA from one normal piece of brain tissue were cloned and sequenced (45 clones). A variety of sequences similar to ERV9 (75 to 93%) were identified. Amplification products were immobilized on nylon filters and hybridized to four different synthetic oligonucleotides derived from the sequenced clones. Sequences without the stop codon seen in ERV9 in this region, possibly encoding functional immunosuppressive proteins, were present in RNA amplificates from all samples. The various cell types showed different hybridization patterns with the four probes. The open reading frame sequences were identified in genomic Southern blots, one probe detecting about 10 copies and another detecting a single copy. Northern (RNA) blots of mRNA from various normal human tissues revealed 2.5-kb (e.g., lung) and 10-kb (e.g., placenta) transcripts hybridizing to one of the probes.

Full text

PDF
1122

Images in this article

1123Image
on p.1123
1124Image
on p.1124
1125Image
on p.1125
1125Image
on p.1125

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benomar A., Ming W. J., Taraboletti G., Ghezzi P., Balotta C., Cianciolo G. J., Snyderman R., Doré J. F., Mantovani A. Chemotactic factor and P15E-related chemotaxis inhibitor in human melanoma cell lines with different macrophage content and tumorigenicity in nude mice. J Immunol. 1987 Apr 1;138(7):2372–2379. [PubMed] [Google Scholar]
  2. Bohannon R. C., Donehower L. A., Ford R. J. Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS. J Virol. 1991 Nov;65(11):5663–5672. doi: 10.1128/jvi.65.11.5663-5672.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cianciolo G. J. Antiinflammatory proteins associated with human and murine neoplasms. Biochim Biophys Acta. 1986 Aug 5;865(1):69–82. doi: 10.1016/0304-419x(86)90014-4. [DOI] [PubMed] [Google Scholar]
  4. Cianciolo G. J., Copeland T. D., Oroszlan S., Snyderman R. Inhibition of lymphocyte proliferation by a synthetic peptide homologous to retroviral envelope proteins. Science. 1985 Oct 25;230(4724):453–455. doi: 10.1126/science.2996136. [DOI] [PubMed] [Google Scholar]
  5. Cianciolo G. J., Matthews T. J., Bolognesi D. P., Snyderman R. Macrophage accumulation in mice is inhibited by low molecular weight products from murine leukemia viruses. J Immunol. 1980 Jun;124(6):2900–2905. [PubMed] [Google Scholar]
  6. Cianciolo G. J., Phipps D., Snyderman R. Human malignant and mitogen-transformed cells contain retroviral P15E-related antigen. J Exp Med. 1984 Mar 1;159(3):964–969. doi: 10.1084/jem.159.3.964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cianciolo G., Hunter J., Silva J., Haskill J. S., Snyderman R. Inhibitors of monocyte responses to chemotaxins are present in human cancerous effusions and react with monoclonal antibodies to the P15(E) structural protein of retroviruses. J Clin Invest. 1981 Oct;68(4):831–844. doi: 10.1172/JCI110338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Copelan E. A., Rinehart J. J., Lewis M., Mathes L., Olsen R., Sagone A. The mechanism of retrovirus suppression of human T cell proliferation in vitro. J Immunol. 1983 Oct;131(4):2017–2020. [PubMed] [Google Scholar]
  9. Devaux J., Buendia M. A., Hamelin R., Bernard C., Tavitian A. Characterization of a large genomic size Moloney murine sarcoma virus produced by a transformed rat cell line. J Gen Virol. 1982 Nov;63(Pt 1):223–226. doi: 10.1099/0022-1317-63-1-223. [DOI] [PubMed] [Google Scholar]
  10. Gottlieb R. A., Kleinerman E. S., O'Brian C. A., Tsujimoto S., Cianciolo G. J., Lennarz W. J. Inhibition of protein kinase C by a peptide conjugate homologous to a domain of the retroviral protein p15E. J Immunol. 1990 Oct 15;145(8):2566–2570. [PubMed] [Google Scholar]
  11. Harris D. T., Cianciolo G. J., Snyderman R., Argov S., Koren H. S. Inhibition of human natural killer cell activity by a synthetic peptide homologous to a conserved region in the retroviral protein, p15E. J Immunol. 1987 Feb 1;138(3):889–894. [PubMed] [Google Scholar]
  12. Hebebrand L. C., Olsen R. G., Mathes L. E., Nichols W. S. Inhibition of human lymphocyte mitogen and antigen response by a 15,000-dalton protein from feline leukemia virus. Cancer Res. 1979 Feb;39(2 Pt 1):443–447. [PubMed] [Google Scholar]
  13. Jacquemin P. C., Strijckmans P., Thiry L. Study of the expression of a glycoprotein of retroviral origin in the plasma of patients with hematologic disorders and in the plasma of normal individuals. Blood. 1984 Oct;64(4):832–841. [PubMed] [Google Scholar]
  14. Kato N., Pfeifer-Ohlsson S., Kato M., Larsson E., Rydnert J., Ohlsson R., Cohen M. Tissue-specific expression of human provirus ERV3 mRNA in human placenta: two of the three ERV3 mRNAs contain human cellular sequences. J Virol. 1987 Jul;61(7):2182–2191. doi: 10.1128/jvi.61.7.2182-2191.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kleinerman E. S., Lachman L. B., Knowles R. D., Snyderman R., Cianciolo G. J. A synthetic peptide homologous to the envelope proteins of retroviruses inhibits monocyte-mediated killing by inactivating interleukin 1. J Immunol. 1987 Oct 1;139(7):2329–2337. [PubMed] [Google Scholar]
  16. La Mantia G., Maglione D., Pengue G., Di Cristofano A., Simeone A., Lanfrancone L., Lania L. Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells. Nucleic Acids Res. 1991 Apr 11;19(7):1513–1520. doi: 10.1093/nar/19.7.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Larsson E., Kato N., Cohen M. Human endogenous proviruses. Curr Top Microbiol Immunol. 1989;148:115–132. doi: 10.1007/978-3-642-74700-7_4. [DOI] [PubMed] [Google Scholar]
  18. Nelson M., Nelson D. S., Cianciolo G. J., Snyderman R. Effects of CKS-17, a synthetic retroviral envelope peptide, on cell-mediated immunity in vivo: immunosuppression, immunogenicity, and relation to immunosuppressive tumor products. Cancer Immunol Immunother. 1989;30(2):113–118. doi: 10.1007/BF01665962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oda T., Ikeda S., Watanabe S., Hatsushika M., Akiyama K., Mitsunobu F. Molecular cloning, complete nucleotide sequence, and gene structure of the provirus genome of a retrovirus produced in a human lymphoblastoid cell line. Virology. 1988 Dec;167(2):468–476. [PubMed] [Google Scholar]
  20. Ono M., Yasunaga T., Miyata T., Ushikubo H. Nucleotide sequence of human endogenous retrovirus genome related to the mouse mammary tumor virus genome. J Virol. 1986 Nov;60(2):589–598. doi: 10.1128/jvi.60.2.589-598.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Repaske R., Steele P. E., O'Neill R. R., Rabson A. B., Martin M. A. Nucleotide sequence of a full-length human endogenous retroviral segment. J Virol. 1985 Jun;54(3):764–772. doi: 10.1128/jvi.54.3.764-772.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ruegg C. L., Monell C. R., Strand M. Identification, using synthetic peptides, of the minimum amino acid sequence from the retroviral transmembrane protein p15E required for inhibition of lymphoproliferation and its similarity to gp21 of human T-lymphotropic virus types I and II. J Virol. 1989 Aug;63(8):3250–3256. doi: 10.1128/jvi.63.8.3250-3256.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  24. Seiki M., Hattori S., Hirayama Y., Yoshida M. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618–3622. doi: 10.1073/pnas.80.12.3618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shih A., Misra R., Rush M. G. Detection of multiple, novel reverse transcriptase coding sequences in human nucleic acids: relation to primate retroviruses. J Virol. 1989 Jan;63(1):64–75. doi: 10.1128/jvi.63.1.64-75.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shinnick T. M., Lerner R. A., Sutcliffe J. G. Nucleotide sequence of Moloney murine leukaemia virus. Nature. 1981 Oct 15;293(5833):543–548. doi: 10.1038/293543a0. [DOI] [PubMed] [Google Scholar]
  27. Sonigo P., Barker C., Hunter E., Wain-Hobson S. Nucleotide sequence of Mason-Pfizer monkey virus: an immunosuppressive D-type retrovirus. Cell. 1986 May 9;45(3):375–385. doi: 10.1016/0092-8674(86)90323-5. [DOI] [PubMed] [Google Scholar]
  28. Wang J. M., Chen Z. G., Cianciolo G. J., Snyderman R., Breviario F., Dejana E., Mantovani A. Production of a retroviral P15E-related chemotaxis inhibitor by IL-1-treated endothelial cells. A possible negative feedback in the regulation of the vascular response to monokines. J Immunol. 1989 Mar 15;142(6):2012–2017. [PubMed] [Google Scholar]
  29. Wang J. M., Cianciolo G. J., Snyderman R., Mantovani A. Coexistence of a chemotactic factor and a retroviral P15E-related chemotaxis inhibitor in human tumor cell culture supernatants. J Immunol. 1986 Oct 15;137(8):2726–2732. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES