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. 2001 Sep 1;85(9):1372–1382. doi: 10.1054/bjoc.2001.2074

Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization

M J J G Stassar 1,1, G Devitt 1, M Brosius 1, L Rinnab 3, J Prang 3, T Schradin 3, J Simon 3, S Petersen 4,2, A Kopp-Schneider 6, M Zöller 1,7
PMCID: PMC2375251  PMID: 11720477

Abstract

Renal cell carcinoma (RCC) are frequently chemo- and radiation resistant. Thus, there is a need for identifying biological features of these cells that could serve as alternative therapeutic targets. We performed suppression subtractive hybridization (SSH) on patient-matched normal renal and RCC tissue to identify variably regulated genes. 11 genes were strongly up-regulated or selectively expressed in more than one RCC tissue or cell line. Screening of filters containing cancer-related cDNAs confirmed overexpression of 3 of these genes and 3 additional genes were identified. These 14 differentially expressed genes, only 6 of which have previously been associated with RCC, are related to tumour growth/survival (EGFR, cyclin D1, insulin-like growth factor-binding protein-1 and a MLRQ sub-unit homologue of the NADH:ubiquinone oxidoreductase complex), angiogenesis (vascular endothelial growth factor, endothelial PAS domain protein-1, ceruloplasmin, angiopoietin-related protein 2) and cell adhesion/motility (protocadherin 2, cadherin 6, autotaxin, vimentin, lysyl oxidase and semaphorin G). Since some of these genes were overexpressed in 80–90% of RCC tissues, it is important to evaluate their suitability as therapeutic targets. © 2001 Cancer Research Campaign

Keywords: human, renal cell carcinoma, gene expression

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Selected References

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