Peptide receptor radiotherapy: a new option for the management of aggressive fibromatosis on behalf of the Italian Sarcoma Group

Abstract

The management of aggressive fibromatosis (AF) is problematic, and few options are available to patients unsuitable for surgery and resistant to external-beam radiation therapy (EBRT). We report on two patients with fast-growing recurrences of AF resistant to EBRT who obtained protracted clinical benefits with ⁹⁰Y-DOTATOC. ⁹⁰Y-DOTATOC should be further investigated in this setting.

Peptide receptor radiotherapy, which uses radiolabelled peptides that recognise tumour cell surface receptors, is a relatively new technique for delivering large quantities of radioactivity to tumours. The somatostatin analogue [DOTA⁰, Tyr³]octreotide (DOTATOC) has been shown to be useful for this purpose, as it is easily and stably labelled with yttrium-90. It has high affinity for the somatostatin receptor subtype 2 (sst2) and a favourable bio-distribution and safety profile in patients (Kwekkeboom et al, 2000). Tumour uptake of ⁹⁰Y-DOTATOC can be revealed before treatment by scintigraphy with ¹¹¹In-labelled octreotide (OctreoScan) (Krenning et al, 1993). When ⁹⁰Y-DOTATOC is given to patients with cancers that express somatostatin receptors and show adequate OctreoScan uptake, a good tumour–response rate is observed (Cremonesi et al, 1999; Waldherr et al, 2001; Chinol et al, 2002).

Aggressive fibromatosis (AF) is a rare fibrous neoplasm with biological behaviour intermediate between that of benign fibrous lesions and malignant fibrosarcomas. The tumour is poorly circumscribed and grows along tissue planes with an infiltrating-like pattern towards mesenchymal tissue. The clinical equivalent to this pathological behaviour is a high tendency to local recurrence. Its management is problematic: adequate surgery is the first-choice treatment; however, the lesion tends to recur locally even if surgically removed with pathologically documented free-margin resection. As systemic treatment options, chemotherapy, hormone therapy and NSAIDs have been used with mixed success.

The response of AF to external-beam radiation (EBRT) is documented, with a dose–response relation for tumour doses of less than 50 Gy (Greenberg et al, 1981; Nuyttens et al, 2000). However, there are AF cases that cannot be managed with EBRT because of normal-tissue constraints, as there are cases that appear to be resistant or refractory to EBRT.

According to these data, and to a report of partial AF regression after prolonged treatment with the somatostatin-analogue lanreotide (Mannant et al, 1995), we decided to investigate the usefulness of ⁹⁰Y-DOTATOC as a treatment for recurrent AF.

MATERIALS AND METHODS

Aggressive fibromatosis specimens from 10 patients were retrospectively examined for immunopositivity to a rabbit polyclonal antibody anti-sst2A (cod SS-800, Biotrend Chemikalien, Germany). Two specimens showed immunoreactivity to sst2. Next, we used OctreoScan in six other patients with AF to test the expression of sst2 receptors on the neoplasm.

Two patients had AF lesions that showed adequate OctreoScan uptake (i.e., at least equal to one of the liver) and were hence eligible for treatment with ⁹⁰Y-DOTATOC. We explained to these two patients the potential risks and benefits of this experimental treatment, which had been approved by our Institute's ethical review board. They gave their written consent. An ongoing phase I–II study using ⁹⁰Y-DOTATOC in patients with solid tumours (Paganelli et al, 2001; Chinol et al, 2002; Bodei et al, 2003) was followed for these patients.

According to this protocol, patients had histologically confirmed cancers expressing sst₂ receptors, as documented by OctreoScan, and documented residual disease or recurrence after conventional treatment. DOTATOC was synthesised at the Division of Radiological Chemistry, University Hospital, Basel using a published procedure (Heppeler et al, 1999). Yttrium-90 chloride was purchased from AEA Technology (Harwell, UK). DOTATOC molecule was radiolabelled according to a published procedure (Chinol et al, 2002). ⁹⁰Y-DOTATOC was injected intravenously over 20 min in 100 ml of physiological saline. Patients received renal protection by infusing 10 g lysine hydrochloride in 500 ml of saline over an hour before therapy and 15 g of lysine hydrochloride in 750 ml saline over 2 h after therapy. The patients were hospitalised for 2–3 days after treatment in rooms set aside for radionuclide therapy, and were discharged only after the level of specific activity in the urine had fallen below 0.037 MBq ml⁻¹, as stipulated in the regulations governing use of radionuclides at our centre.

Toxicity was evaluated according to WHO criteria (Miller et al, 1981). After discharge from hospital the patients underwent the following tests: renal function, hepatic function, LDH and uricaemia every 30 days, complete blood count every 15 days for the first 2 months and monthly subsequently.

RESULTS

Case 1

The first patient, a 30-year-old woman, had a local relapse of right-thigh AF. Nine months after the primary tumour had been radically removed, she underwent further surgery for a local relapse; however, during subsequent adjuvant external-beam radiotherapy the disease again recurred and grew rapidly. The patient received i.v., ⁹⁰Y-DOTATOC in five cycles over 14 months, cumulative activity 10.6 GBq. There was a partial response on MRI according to WHO criteria after the first cycle (2.22 GBq) which has lasted for more than 37 months (Figures 1 and 2).

Figure 1.

The patient had a local relapse of right-thigh aggressive fibromatosis: a scintigraphy with ¹¹¹In-labelled octreotide (OctreoScan) revealed tumour uptake of the radiopeptide (arrow).

Figure 2.

(A) Magnetic resonance (SE FS T1W post Gd-DTPA Image) showed a local relapse of right-thigh aggressive fibromatosis (arrow). (B) A partial response of disease (WHO criteria) was obtained by treating with ⁹⁰Y-DOTATOC (SE T1W post Gd-DTPA magnetic resonance image).

Case 2

The second patient was a 22-year-old man with AF of the right thigh. After radical surgery and adjuvant radiotherapy (44 Gy external-beam and 27 Gy brachytherapy), there were four relapses in the following 4 years. The relapses were removed surgically and postoperative external-beam reirradiation (35 Gy) was given after the third operation.

For the fifth relapse, characterised by rapid growth, conservative surgery and radiotherapy were no longer possible, and as an alternative to amputation we administered a cumulative activity of 8.18 GBq of ⁹⁰Y-DOTATOC in three cycles over 4 months. There was a disease stabilisation on MRI according to WHO criteria after the first cycle (3.26 GBq) and disease has remained stable for more than 19 months.

⁹⁰Y-DOTATOC was very well tolerated in these patients, and neither acute nor late toxicity was observed.

DISCUSSION

Patients with recurrent AF after full exploitation of the standard therapy modalities such as surgery and EBRT are lacking other local therapeutic options. In this communication, we report on two patients with fast-growing recurrence of AF that appeared to be resistant to appropriate EBRT.

Both patients obtained a protracted clinical benefit with a peptide receptor radiotherapy with ⁹⁰Y-DOTATOC in the absence of acute and late toxicity. The excellent tolerability of this experimental treatment is consistent with the results of our phase I–II study (Paganelli et al, 2001; Chinol et al, 2002; Bodei et al, in press). In this study, toxicity due to ⁹⁰Y-DOTATOC, which was mainly haematological, occurred in a minority of patients receiving high activities per cycle (grade III toxicity in three out of seven — 43% — of the patients receiving 5.18 GBq, defined as MTD/cycle) and tended to reverse within 4 weeks. Neither permanent renal toxicity nor other forms of toxicity were observed.

To our knowledge, this is the first report showing that ⁹⁰Y-DOTATOC can provide clinical benefits in patients with AF, and the benefit obtained supports further investigation in this setting. An OctreoScan should first be performed on a candidate patient with AF and if adequate tumour uptake is observed, ⁹⁰Y-DOTATOC could be considered as a further treatment option to increase the chance of obtaining disease control.