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. 1993 Jun;67(6):3087–3094. doi: 10.1128/jvi.67.6.3087-3094.1993

Epstein-Barr virus latent membrane protein 2A blocks calcium mobilization in B lymphocytes.

C L Miller 1, R Longnecker 1, E Kieff 1
PMCID: PMC237645  PMID: 7684459

Abstract

LMP2A is expressed in latent Epstein-Barr virus (EBV) infection and interacts with LMP1 and members of the src tyrosine kinase family in the plasma membrane. Since tyrosine kinase mediate receptor-induced changes in intracellular free calcium, the effect of LMP2A on receptor-mediated intracellular calcium mobilization was evaluated by stably expressing LMP2A in an EBV-negative Burkitt tumor cell line (BJAB) or in LMP1-converted BJAB cells. LMP2A significantly blocked calcium mobilization following class II, CD19, or immunoglobulin M cross-linking. LMP2A effects were partially reversed in LMP1-converted cell lines. These results are compatible with LMP2A acting in latent B-lymphocyte infection to downmodulate LMP1 effects on cell growth or to inhibit induction of lytic EBV infection in specific human tissues following receptor ligation.

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Selected References

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