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. 1993 Jun;67(6):3191–3198. doi: 10.1128/jvi.67.6.3191-3198.1993

Hybrid human immunodeficiency virus Gag particles as an antigen carrier system: induction of cytotoxic T-cell and humoral responses by a Gag:V3 fusion.

J C Griffiths 1, S J Harris 1, G T Layton 1, E L Berrie 1, T J French 1, N R Burns 1, S E Adams 1, A J Kingsman 1
PMCID: PMC237658  PMID: 8497047

Abstract

In attempts to increase the immunogenicity of recombinant antigens, a number of particulate antigen presentation systems have been developed. In this study, we used human immunodeficiency virus Gag particles as carriers for the human immunodeficiency virus envelope V3 region. Gag:V3 fusion proteins were expressed from baculovirus expression vectors; they migrated to the insect cell membrane and budded from the cells as hybrid particles. An immunization study carried out with rats showed that the particles elicited a strong anti-Gag antibody response and a weak antibody response to the V3 region. A strong anti-V3 cytolytic T-cell response was elicited in immunized mice. These data show that retroviral Gag particles can be used as antigen presentation vehicles.

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Selected References

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