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. 1991;102:195–224.

The Jeremiah Metzger Lecture. From POMC to functional diversity of neural peptides: the key importance of convertases.

M Chretien 1, L Gasper 1, S Benjannet 1, M Mbikay 1, C Lazure 1, N G Seidah 1
PMCID: PMC2376649  PMID: 1966644

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Selected References

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  1. Al-Dujaili E. A., Hope J., Estivariz F. E., Lowry P. J., Edwards C. R. Circulating human pituitary pro-gamma-melanotropin enhances the adrenal response to ACTH. Nature. 1981 May 14;291(5811):156–159. doi: 10.1038/291156a0. [DOI] [PubMed] [Google Scholar]
  2. Amara S. G., Jonas V., Rosenfeld M. G., Ong E. S., Evans R. M. Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature. 1982 Jul 15;298(5871):240–244. doi: 10.1038/298240a0. [DOI] [PubMed] [Google Scholar]
  3. BRANSOME E. D., Jr, REDDY W. J. Studies of adrenal nucleic acids: the influence of ACTH, unilateral adrenalectomy and growth hormone upon adrenal RNA and DNA in the dog. Endocrinology. 1961 Dec;69:997–1008. doi: 10.1210/endo-69-6-997. [DOI] [PubMed] [Google Scholar]
  4. Boileau G., Barbeau C., Jeannotte L., Chrétien M., Drouin J. Complete structure of the porcine pro-opiomelanocortin mRNA derived from the nucleotide sequence of cloned cDNA. Nucleic Acids Res. 1983 Nov 25;11(22):8063–8071. doi: 10.1093/nar/11.22.8063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Böhlen P., Wehrenberg W. B., Esch F., Ling N., Brazeau P., Guillemin R. Rat hypothalamic growth hormone-releasing factor: isolation, sequence analysis and total synthesis. Biochem Biophys Res Commun. 1984 Dec 28;125(3):1005–1012. doi: 10.1016/0006-291x(84)91383-4. [DOI] [PubMed] [Google Scholar]
  6. CATER D. B., STACK-DUNNE M. P. The effects of growth hormone and corticotrophin upon the adrenal weight and adrenocortical mitotic activity in the hypophysectomized rat. J Endocrinol. 1955 May;12(3):174–184. doi: 10.1677/joe.0.0120174. [DOI] [PubMed] [Google Scholar]
  7. CATER D. B., STACK-DUNNE M. P. The histological changes in the adrenal of the hypophysectomised rat after treatment with pituitary preparations. J Pathol Bacteriol. 1953 Jul;66(1):119–133. [PubMed] [Google Scholar]
  8. Chan J. S., Seidah N. G., Chrétien M. Measurement of N-terminal (1-76) of human proopiomelanocortin in human plasma: correlation with adrenocorticotropin. J Clin Endocrinol Metab. 1983 Apr;56(4):791–796. doi: 10.1210/jcem-56-4-791. [DOI] [PubMed] [Google Scholar]
  9. Chang A. C., Cochet M., Cohen S. N. Structural organization of human genomic DNA encoding the pro-opiomelanocortin peptide. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4890–4894. doi: 10.1073/pnas.77.8.4890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Charlton B. G. Adrenal cortical innervation and glucocorticoid secretion. J Endocrinol. 1990 Jul;126(1):5–8. doi: 10.1677/joe.0.1260005. [DOI] [PubMed] [Google Scholar]
  11. Chaudhari N., Hahn W. E. Genetic expression in the developing brain. Science. 1983 May 27;220(4600):924–928. doi: 10.1126/science.6189184. [DOI] [PubMed] [Google Scholar]
  12. Chrétien M., Benjannet S., Gossard F., Gianoulakis C., Crine P., Lis M., Seidah N. G. From beta-lipotropin to beta-endorphin and 'pro-opio-melanocortin'. Can J Biochem. 1979 Sep;57(9):1111–1121. doi: 10.1139/o79-143. [DOI] [PubMed] [Google Scholar]
  13. Clamagirand C., Camier M., Boussetta H., Fahy C., Morel A., Nicolas P., Cohen P. An endopeptidase associated with bovine neurohypophysis secretory granules cleaves pro-ocytocin/neurophysin peptide at paired basic residues. Biochem Biophys Res Commun. 1986 Feb 13;134(3):1190–1196. doi: 10.1016/0006-291x(86)90376-1. [DOI] [PubMed] [Google Scholar]
  14. Comb M., Seeburg P. H., Adelman J., Eiden L., Herbert E. Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA. Nature. 1982 Feb 25;295(5851):663–666. doi: 10.1038/295663a0. [DOI] [PubMed] [Google Scholar]
  15. Crickard K., Fujii D. K., Jaffe R. B. Isolation and identification of human fetal adrenal medullary cells in vitro. J Clin Endocrinol Metab. 1982 Dec;55(6):1143–1148. doi: 10.1210/jcem-55-6-1143. [DOI] [PubMed] [Google Scholar]
  16. Crickard K., Ill C. R., Jaffe R. B. Control of proliferation of human fetal adrenal cells in vitro. J Clin Endocrinol Metab. 1981 Oct;53(4):790–796. doi: 10.1210/jcem-53-4-790. [DOI] [PubMed] [Google Scholar]
  17. Crine P., Gianoulakis C., Seidah N. G., Gossard F., Pezalla P. D., Lis M., Chrétien M. Biosynthesis of beta-endorphin from beta-lipotropin and a larger molecular weight precursor in rat pars intermedia. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4719–4723. doi: 10.1073/pnas.75.10.4719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. DIXON H. B. F., MOORE S., STACK-DUNNE M. P., YOUNG F. G. Chromatography of adrenotropic hormone on ion-exchange columns. Nature. 1951 Dec 15;168(4285):1044–1045. doi: 10.1038/1681044a0. [DOI] [PubMed] [Google Scholar]
  19. Dallman M. F., Engeland W. C., Rose J. C., Wilkinson C. W., Shinsako J., Siedenburg F. Nycthemeral rhythm in adrenal responsiveness to ACTH. Am J Physiol. 1978 Nov;235(5):R210–R218. doi: 10.1152/ajpregu.1978.235.5.R210. [DOI] [PubMed] [Google Scholar]
  20. Dallman M. F., Engeland W. C., Shinsako J. Compensatory adrenal growth: a neurally mediated reflex. Am J Physiol. 1976 Aug;231(2):408–414. doi: 10.1152/ajplegacy.1976.231.2.408. [DOI] [PubMed] [Google Scholar]
  21. Drouin J., Goodman H. M. Most of the coding region of rat ACTH beta--LPH precursor gene lacks intervening sequences. Nature. 1980 Dec 11;288(5791):610–613. doi: 10.1038/288610a0. [DOI] [PubMed] [Google Scholar]
  22. Elliott T. R. The innervation of the adrenal glands. J Physiol. 1913 Jun 19;46(3):285–290. doi: 10.1113/jphysiol.1913.sp001591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Estivariz F. E., Birch N. P., Loh Y. P. Generation of Lys-gamma 3-melanotropin from pro-opiomelanocortin 1-77 by a bovine intermediate lobe secretory vesicle membrane-associated aspartic protease and purified pro-opiomelanocortin converting enzyme. J Biol Chem. 1989 Oct 25;264(30):17796–17801. [PubMed] [Google Scholar]
  24. Estivariz F. E., Carino M., Lowry P. J., Jackson S. Further evidence that N-terminal pro-opiomelanocortin peptides are involved in adrenal mitogenesis. J Endocrinol. 1988 Feb;116(2):201–206. doi: 10.1677/joe.0.1160201. [DOI] [PubMed] [Google Scholar]
  25. Estivariz F. E., Iturriza F., McLean C., Hope J., Lowry P. J. Stimulation of adrenal mitogenesis by N-terminal proopiocortin peptides. Nature. 1982 Jun 3;297(5865):419–422. doi: 10.1038/297419a0. [DOI] [PubMed] [Google Scholar]
  26. Estivariz F. E., Morano M. I., Carino M., Jackson S., Lowry P. J. Adrenal regeneration in the rat is mediated by mitogenic N-terminal pro-opiomelanocortin peptides generated by changes in precursor processing in the anterior pituitary. J Endocrinol. 1988 Feb;116(2):207–216. doi: 10.1677/joe.0.1160207. [DOI] [PubMed] [Google Scholar]
  27. Fletcher D. J., Quigley J. P., Bauer G. E., Noe B. D. Characterization of proinsulin- and proglucagon-converting activities in isolated islet secretory granules. J Cell Biol. 1981 Aug;90(2):312–322. doi: 10.1083/jcb.90.2.312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Fuller R. S., Brake A. J., Thorner J. Intracellular targeting and structural conservation of a prohormone-processing endoprotease. Science. 1989 Oct 27;246(4929):482–486. doi: 10.1126/science.2683070. [DOI] [PubMed] [Google Scholar]
  29. Gagner J. P., Drouin J. Tissue-specific regulation of pituitary proopiomelanocortin gene transcription by corticotropin-releasing hormone, 3',5'-cyclic adenosine monophosphate, and glucocorticoids. Mol Endocrinol. 1987 Oct;1(10):677–682. doi: 10.1210/mend-1-10-677. [DOI] [PubMed] [Google Scholar]
  30. Gaspar L., Chan J. S., Seidah N. G., Chrétien M. Characterization of N-terminal fragment of proopiomelanocortin in cerebrospinal fluid. J Clin Endocrinol Metab. 1987 Jul;65(1):198–202. doi: 10.1210/jcem-65-1-198. [DOI] [PubMed] [Google Scholar]
  31. Gaspar L., Chan J. S., Seidah N. G., Chrétien M. Peptides related to the N-terminus of pro-opiomelanocortin in the human adrenal medulla. Clin Invest Med. 1989 Apr;12(2):90–98. [PubMed] [Google Scholar]
  32. Gaspar L., Chan J. S., Seidah N. G., Chrétien M. Urinary molecular forms of human N-terminal of proopiomelanocortin: possible deglycosylation and degradation by the kidney. J Clin Endocrinol Metab. 1984 Oct;59(4):614–621. doi: 10.1210/jcem-59-4-614. [DOI] [PubMed] [Google Scholar]
  33. Gaspar L., Gilbert F., Chan J. S., Seidah N. G., Chrétien M. Urinary levels of immunoreactive NH2-terminal of pro-opiomelanocortin in patients with malignant pulmonary disease. Clin Invest Med. 1989 Aug;12(4):241–246. [PubMed] [Google Scholar]
  34. Gewirtz G., Yalow R. S. Ectopic ACTH production in carcinoma of the lung. J Clin Invest. 1974 Apr;53(4):1022–1032. doi: 10.1172/JCI107639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Gill G. N. Mechanism of ACTH action. Metabolism. 1972 Jun;21(6):571–588. doi: 10.1016/0026-0495(72)90101-1. [DOI] [PubMed] [Google Scholar]
  36. Gospodarowicz D., Handley H. H. Stimulation of division of Y1 adrenal cells by a growth factor isolated from bovine pituitary glands. Endocrinology. 1975 Jul;97(1):102–107. doi: 10.1210/endo-97-1-102. [DOI] [PubMed] [Google Scholar]
  37. Guillemin R., Ling N., Burgus R., Bloom F., Segal D. Characterization of the endorphins, novel hypothalamic and neurohypophysial peptides with opiate-like activity: evidence that they induce profound behavioral changes. Psychoneuroendocrinology. 1977;2(1):59–62. doi: 10.1016/0306-4530(77)90032-4. [DOI] [PubMed] [Google Scholar]
  38. Güllner H. G., Nicholson W. E., Gill J. R., Jr, Orth D. N. Plasma immunoreactive proopiolipomelanocortin-derived peptides in patients with primary hyperaldosteronism, idiopathic hyperaldosteronism with bilateral adrenal hyperplasia, and dexamethasone-suppressible hyperaldosteronism. J Clin Endocrinol Metab. 1983 Apr;56(4):853–855. doi: 10.1210/jcem-56-4-853. [DOI] [PubMed] [Google Scholar]
  39. Han V. K., D'Ercole A. J., Lund P. K. Cellular localization of somatomedin (insulin-like growth factor) messenger RNA in the human fetus. Science. 1987 Apr 10;236(4798):193–197. doi: 10.1126/science.3563497. [DOI] [PubMed] [Google Scholar]
  40. Holzwarth M. A., Cunningham L. A., Kleitman N. The role of adrenal nerves in the regulation of adrenocortical functions. Ann N Y Acad Sci. 1987;512:449–464. doi: 10.1111/j.1749-6632.1987.tb24980.x. [DOI] [PubMed] [Google Scholar]
  41. Hökfelt T., Elfvin L. G., Elde R., Schultzberg M., Goldstein M., Luft R. Occurrence of somatostatin-like immunoreactivity in some peripheral sympathetic noradrenergic neurons. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3587–3591. doi: 10.1073/pnas.74.8.3587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Hökfelt T., Johansson O., Ljungdahl A., Lundberg J. M., Schultzberg M. Peptidergic neurones. Nature. 1980 Apr 10;284(5756):515–521. doi: 10.1038/284515a0. [DOI] [PubMed] [Google Scholar]
  43. JAILER J. W., LONGSON D., CHRISTY N. P. Studies in Cushing's syndrome. II. Adrenal weight-maintaining activity in the plasma of patients with Cushing's syndrome. J Clin Invest. 1957 Nov;36(11):1608–1614. doi: 10.1172/JCI103560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Jaffe R. B., Mulchahey J. J., Di Blasio A. M., Martin M. C., Blumenfeld Z., Dumesic D. A. Peptide regulation of pituitary and target tissue function and growth in the primate fetus. Recent Prog Horm Res. 1988;44:431–549. doi: 10.1016/b978-0-12-571144-9.50017-x. [DOI] [PubMed] [Google Scholar]
  45. Jost A. Problems of fetal endocrinology: the adrenal glands. Recent Prog Horm Res. 1966;22:541–574. doi: 10.1016/b978-1-4831-9825-5.50017-8. [DOI] [PubMed] [Google Scholar]
  46. Julius D., Brake A., Blair L., Kunisawa R., Thorner J. Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-alpha-factor. Cell. 1984 Jul;37(3):1075–1089. doi: 10.1016/0092-8674(84)90442-2. [DOI] [PubMed] [Google Scholar]
  47. Kakidani H., Furutani Y., Takahashi H., Noda M., Morimoto Y., Hirose T., Asai M., Inayama S., Nakanishi S., Numa S. Cloning and sequence analysis of cDNA for porcine beta-neo-endorphin/dynorphin precursor. Nature. 1982 Jul 15;298(5871):245–249. doi: 10.1038/298245a0. [DOI] [PubMed] [Google Scholar]
  48. Kakidani H., Furutani Y., Takahashi H., Noda M., Morimoto Y., Hirose T., Asai M., Inayama S., Nakanishi S., Numa S. Cloning and sequence analysis of cDNA for porcine beta-neo-endorphin/dynorphin precursor. Nature. 1982 Jul 15;298(5871):245–249. doi: 10.1038/298245a0. [DOI] [PubMed] [Google Scholar]
  49. Krieger D. T. Physiopathology of Cushing's disease. Endocr Rev. 1983 Winter;4(1):22–43. doi: 10.1210/edrv-4-1-22. [DOI] [PubMed] [Google Scholar]
  50. Krieger D. T. Pituitary hormones in the brain: what is their function? Fed Proc. 1980 Sep;39(11):2937–2941. [PubMed] [Google Scholar]
  51. Lechan R. M., Wu P., Jackson I. M., Wolf H., Cooperman S., Mandel G., Goodman R. H. Thyrotropin-releasing hormone precursor: characterization in rat brain. Science. 1986 Jan 10;231(4734):159–161. doi: 10.1126/science.3079917. [DOI] [PubMed] [Google Scholar]
  52. Li C. H., Chung D. Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1145–1148. doi: 10.1073/pnas.73.4.1145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  53. Liotta A. S., Krieger D. T. In vitro biosynthesis and comparative posttranslational processing of immunoreactive precursor corticotropin/beta-endorphin by human placental and pituitary cells. Endocrinology. 1980 May;106(5):1504–1511. doi: 10.1210/endo-106-5-1504. [DOI] [PubMed] [Google Scholar]
  54. Loh Y. P., Parish D. C., Tuteja R. Purification and characterization of a paired basic residue-specific pro-opiomelanocortin converting enzyme from bovine pituitary intermediate lobe secretory vesicles. J Biol Chem. 1985 Jun 25;260(12):7194–7205. [PubMed] [Google Scholar]
  55. Lowry P. J., Silas L., McLean C., Linton E. A., Estivariz F. E. Pro-gamma-melanocyte-stimulating hormone cleavage in adrenal gland undergoing compensatory growth. Nature. 1983 Nov 3;306(5938):70–73. doi: 10.1038/306070a0. [DOI] [PubMed] [Google Scholar]
  56. Mains R. E., Eipper B. A., Ling N. Common precursor to corticotropins and endorphins. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3014–3018. doi: 10.1073/pnas.74.7.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  57. Masui H., Garren L. D. Inhibition of replication in functional mouse adrenal tumor cells by adrenocorticotropic hormone mediated by adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3206–3210. doi: 10.1073/pnas.68.12.3206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  58. Masui H., Garren L. D. On the mechanism of action of adrenocorticotropic hormone. Stimulation of deoxyribonucleic acid polymerase and thymidine kinase activities in adrenal glands. J Biol Chem. 1970 May 25;245(10):2627–2632. [PubMed] [Google Scholar]
  59. McKusick V. A. The morbid anatomy of the human genome: a review of gene mapping in clinical medicine (1). Medicine (Baltimore) 1986 Jan;65(1):1–33. doi: 10.1097/00005792-198601000-00001. [DOI] [PubMed] [Google Scholar]
  60. Nakanishi S., Inoue A., Kita T., Nakamura M., Chang A. C., Cohen S. N., Numa S. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature. 1979 Mar 29;278(5703):423–427. doi: 10.1038/278423a0. [DOI] [PubMed] [Google Scholar]
  61. Nawa H., Kotani H., Nakanishi S. Tissue-specific generation of two preprotachykinin mRNAs from one gene by alternative RNA splicing. Nature. 1984 Dec 20;312(5996):729–734. doi: 10.1038/312729a0. [DOI] [PubMed] [Google Scholar]
  62. Orci L., Vassalli J. D., Perrelet A. The insulin factory. Sci Am. 1988 Sep;259(3):85–94. doi: 10.1038/scientificamerican0988-85. [DOI] [PubMed] [Google Scholar]
  63. Orth D. N., Nicholson W. E., Mitchell W. M., Island D. P., Shapiro M., Byyny R. L. ACTH and MSH production by a single cloned mouse pituitary tumor cell line. Endocrinology. 1973 Feb;92(2):385–393. doi: 10.1210/endo-92-2-385. [DOI] [PubMed] [Google Scholar]
  64. Payet N., Lehoux J. G. A comparative study of the role of vasopressin and ACTH in the regulation of growth and function of rat adrenal glands. J Steroid Biochem. 1980 Jan;12:461–467. doi: 10.1016/0022-4731(80)90307-6. [DOI] [PubMed] [Google Scholar]
  65. Phifer R. F., Orth D. N., Spicer S. S. Specific demonstration of the human hypophyseal adrenocortico-melanotropic (ACTH-MSH) cell. J Clin Endocrinol Metab. 1974 Oct;39(4):684–692. doi: 10.1210/jcem-39-4-684. [DOI] [PubMed] [Google Scholar]
  66. Phillips R., Crock C., Funder J. Effects of mineralocorticoids and glucocorticoids on compensatory adrenal growth in rats. Am J Physiol. 1985 Apr;248(4 Pt 1):E450–E456. doi: 10.1152/ajpendo.1985.248.4.E450. [DOI] [PubMed] [Google Scholar]
  67. Powers C. A., Nasjletti A. A novel kinin-generating protease (kininogenase) in the porcine anterior pituitary. J Biol Chem. 1982 May 25;257(10):5594–5600. [PubMed] [Google Scholar]
  68. Ramachandran J., Kong Y. C., Liles S. Effects of ACTH and its O-nitrophenyl sulphenyl derivative on adrenocortical function in vivo. Acta Endocrinol (Copenh) 1976 Jul;82(3):587–599. doi: 10.1530/acta.0.0820587. [DOI] [PubMed] [Google Scholar]
  69. Ramachandran J., Suyama A. T. Inhibition of replication of normal adrenocortical cells in culture by adrenocorticotropin. Proc Natl Acad Sci U S A. 1975 Jan;72(1):113–117. doi: 10.1073/pnas.72.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  70. Rholam M., Nicolas P., Cohen P. Precursors for peptide hormones share common secondary structures forming features at the proteolytic processing sites. FEBS Lett. 1986 Oct 20;207(1):1–6. doi: 10.1016/0014-5793(86)80002-3. [DOI] [PubMed] [Google Scholar]
  71. Robba C., Rebuffat P., Mazzocchi G., Nussdorfer G. G. Long-term trophic action of alpha-melanocyte-stimulating hormone on the zona glomerulosa of the rat adrenal cortex. Acta Endocrinol (Copenh) 1986 Jul;112(3):404–408. doi: 10.1530/acta.0.1120404. [DOI] [PubMed] [Google Scholar]
  72. Robbins D. C., Blix P. M., Rubenstein A. H., Kanazawa Y., Kosaka K., Tager H. S. A human proinsulin variant at arginine 65. Nature. 1981 Jun 25;291(5817):679–681. doi: 10.1038/291679a0. [DOI] [PubMed] [Google Scholar]
  73. Roberts J. L., Herbert E. Characterization of a common precursor to corticotropin and beta-lipotropin: cell-free synthesis of the precursor and identification of corticotropin peptides in the molecule. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4826–4830. doi: 10.1073/pnas.74.11.4826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  74. Robinson P. M., Perry R. A., Hardy K. J., Coghlan J. P., Scoggins B. A. The innervation of the adrenal cortex in the sheep, Ovis ovis. J Anat. 1977 Sep;124(Pt 1):117–129. [PMC free article] [PubMed] [Google Scholar]
  75. Seidah N. G., Cromlish J. A., Hamelin J., Thibault G., Chrétien M. Homologous IRCM-serine protease 1 from pituitary, heart atrium and ventricle: a common pro-hormone maturation enzyme? Biosci Rep. 1986 Sep;6(9):835–844. doi: 10.1007/BF01117107. [DOI] [PubMed] [Google Scholar]
  76. Seidah N. G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., Chrétien M. cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases. DNA Cell Biol. 1990 Jul-Aug;9(6):415–424. doi: 10.1089/dna.1990.9.415. [DOI] [PubMed] [Google Scholar]
  77. Seidah N. G., Rochemont J., Hamelin J., Lis M., Chrétien M. Primary structure of the major human pituitary pro-opiomelanocortin NH2-terminal glycopeptide. Evidence for an aldosterone-stimulating activity. J Biol Chem. 1981 Aug 10;256(15):7977–7984. [PubMed] [Google Scholar]
  78. Smeekens S. P., Steiner D. F. Identification of a human insulinoma cDNA encoding a novel mammalian protein structurally related to the yeast dibasic processing protease Kex2. J Biol Chem. 1990 Feb 25;265(6):2997–3000. [PubMed] [Google Scholar]
  79. Steiner D. F., Cunningham D., Spigelman L., Aten B. Insulin biosynthesis: evidence for a precursor. Science. 1967 Aug 11;157(3789):697–700. doi: 10.1126/science.157.3789.697. [DOI] [PubMed] [Google Scholar]
  80. Taki T. M., Nickerson P. A. Differentiation and proliferation of adrenocortical cells during the early stages of regeneration. Lab Invest. 1985 Jul;53(1):91–100. [PubMed] [Google Scholar]
  81. Tanaka I., Nakai Y., Nakao K., Oki S., Masaki N., Ohtsuki H., Imura H. Presence of immunoreactive gamma-melanocyte-stimulating hormone, adrenocorticotropin, and beta-endorphin in human gastric antral mucosa. J Clin Endocrinol Metab. 1982 Feb;54(2):392–396. doi: 10.1210/jcem-54-2-392. [DOI] [PubMed] [Google Scholar]
  82. Thomas G., Thorne B. A., Thomas L., Allen R. G., Hruby D. E., Fuller R., Thorner J. Yeast KEX2 endopeptidase correctly cleaves a neuroendocrine prohormone in mammalian cells. Science. 1988 Jul 8;241(4862):226–230. doi: 10.1126/science.3291117. [DOI] [PubMed] [Google Scholar]
  83. Uhler M., Herbert E. Complete amino acid sequence of mouse pro-opiomelanocortin derived from the nucleotide sequence of pro-opiomelanocortin cDNA. J Biol Chem. 1983 Jan 10;258(1):257–261. [PubMed] [Google Scholar]
  84. Unsicker K. On the innervation of the rat and pig adrenal cortex. Z Zellforsch Mikrosk Anat. 1971;116(1):151–156. doi: 10.1007/BF00332863. [DOI] [PubMed] [Google Scholar]
  85. Van Ness J., Maxwell I. H., Hahn W. E. Complex population of nonpolyadenylated messenger RNA in mouse brain. Cell. 1979 Dec;18(4):1341–1349. doi: 10.1016/0092-8674(79)90244-7. [DOI] [PubMed] [Google Scholar]
  86. Verdoorn T. A., Draguhn A., Ymer S., Seeburg P. H., Sakmann B. Functional properties of recombinant rat GABAA receptors depend upon subunit composition. Neuron. 1990 Jun;4(6):919–928. doi: 10.1016/0896-6273(90)90145-6. [DOI] [PubMed] [Google Scholar]
  87. Vinson G. P., Pudney J. A., Whitehouse B. J. The mammalian adrenal circulation and the relationship between adrenal blood flow and steroidogenesis. J Endocrinol. 1985 May;105(2):285–294. doi: 10.1677/joe.0.1050285. [DOI] [PubMed] [Google Scholar]
  88. Vinson G. P., Whitehouse B. J., Bateman A., Dell A., Laird S. M. The actions of N-terminal fragments of corticotrophin on steroidogenesis in dispersed rat adrenal cells in vitro. J Endocrinol. 1986 May;109(2):275–278. doi: 10.1677/joe.0.1090275. [DOI] [PubMed] [Google Scholar]
  89. Watson S. J., Akil H., Richard C. W., 3rd, Barchas J. D. Evidence for two separate opiate peptide neuronal systems. Nature. 1978 Sep 21;275(5677):226–228. doi: 10.1038/275226a0. [DOI] [PubMed] [Google Scholar]
  90. de Keyzer Y., Lenne F., Massias J. F., Vieau D., Luton J. P., Kahn A., Bertagna X. Pituitary-like proopiomelanocortin transcripts in human Leydig cell tumors. J Clin Invest. 1990 Sep;86(3):871–877. doi: 10.1172/JCI114787. [DOI] [PMC free article] [PubMed] [Google Scholar]

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