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. 1993 Jun;67(6):3620–3623. doi: 10.1128/jvi.67.6.3620-3623.1993

Many-trillionfold amplification of single RNA virus particles fails to overcome the Muller's ratchet effect.

E A Duarte 1, D K Clarke 1, A Moya 1, S F Elena 1, E Domingo 1, J Holland 1
PMCID: PMC237712  PMID: 8388514

Abstract

We showed earlier that transfers of large populations of RNA viruses lead to fitness gains and that repeated genetic bottleneck transfers result in fitness losses due to Muller's ratchet. In the present study, we examined the effects of genetic bottleneck passages intervening between population passages, a process akin to some natural viral transmissions, using vesicular stomatitis virus as a model. Our findings show that the pronounced fitness increases that occur during two successive population passages cannot overcome the fitness decreases caused by a single intervening genetic bottleneck passage. The implications for natural transmissions of RNA viruses are discussed.

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Selected References

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