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. 1993 Aug;67(8):4760–4768. doi: 10.1128/jvi.67.8.4760-4768.1993

MxA-dependent inhibition of measles virus glycoprotein synthesis in a stably transfected human monocytic cell line.

J J Schnorr 1, S Schneider-Schaulies 1, A Simon-Jödicke 1, J Pavlovic 1, M A Horisberger 1, V ter Meulen 1
PMCID: PMC237862  PMID: 8392613

Abstract

The alpha/beta (type I) interferon-inducible human MxA protein confers resistance to vesicular stomatitis virus (VSV) and influenza A virus in MxA-transfected mouse 3T3 cells (3T3/MxA). We investigated the inhibitory effects of the MxA protein on measles virus (MV) and VSV in the human monocytic cell line U937. In transfected U937 clones which constitutively express MxA (U937/MxA), the release of infectious MV and VSV was reduced approximately 100-fold in comparison with control titers. Transcription of VSV was inhibited similar to that observed for 3T3/MxA cells, whereas no difference was detected for MV in the rates of transcription or the levels of MV-specific mRNAs. In contrast, analysis of MV protein expression by immunofluorescence and immunoprecipitation revealed a significant reduction in the synthesis of MV glycoproteins F and H in U937/MxA cells. These data demonstrate a virus-specific effect of MxA which may, in the case of MV, contribute to the establishment of a persistent infection in human monocytic cells.

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