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. 1993 Sep;67(9):5660–5663. doi: 10.1128/jvi.67.9.5660-5663.1993

The major capsid protein VP2 of minute virus of mice (MVM) can form particles which bind to the 3'-terminal hairpin of MVM replicative-form DNA and package single-stranded viral progeny DNA.

K Willwand 1, B Hirt 1
PMCID: PMC237972  PMID: 8350419

Abstract

The capsids of minute virus of mice (MVM) consist of two closely related proteins, VP1 and VP2. We inactivated the VP1 gene in an infectious clone of MVM DNA by frameshift mutation. After transfection of mutated DNA, capsids consisting of VP2 only were made. They can package negative-strand DNA, and they specifically bind MVM 3'-terminal hairpin DNA.

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Selected References

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  1. Antonietti J. P., Sahli R., Beard P., Hirt B. Characterization of the cell type-specific determinant in the genome of minute virus of mice. J Virol. 1988 Feb;62(2):552–557. doi: 10.1128/jvi.62.2.552-557.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Astell C. R., Gardiner E. M., Tattersall P. DNA sequence of the lymphotropic variant of minute virus of mice, MVM(i), and comparison with the DNA sequence of the fibrotropic prototype strain. J Virol. 1986 Feb;57(2):656–669. doi: 10.1128/jvi.57.2.656-669.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Astell C. R., Smith M., Chow M. B., Ward D. C. Structure of the 3' hairpin termini of four rodent parvovirus genomes: nucleotide sequence homology at origins of DNA replication. Cell. 1979 Jul;17(3):691–703. doi: 10.1016/0092-8674(79)90276-9. [DOI] [PubMed] [Google Scholar]
  4. Bourguignon G. J., Tattersall P. J., Ward D. C. DNA of minute virus of mice: self-priming, nonpermuted, single-stranded genome with a 5'-terminal hairpin duplex. J Virol. 1976 Oct;20(1):290–306. doi: 10.1128/jvi.20.1.290-306.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cotmore S. F., Tattersall P. The autonomously replicating parvoviruses of vertebrates. Adv Virus Res. 1987;33:91–174. doi: 10.1016/s0065-3527(08)60317-6. [DOI] [PubMed] [Google Scholar]
  6. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  7. Jongeneel C. V., Sahli R., McMaster G. K., Hirt B. A precise map of splice junctions in the mRNAs of minute virus of mice, an autonomous parvovirus. J Virol. 1986 Sep;59(3):564–573. doi: 10.1128/jvi.59.3.564-573.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Metcalf J. B., Bates R. C., Lederman M. Interaction of virally coded protein and a cell cycle-regulated cellular protein with the bovine parvovirus left terminus ori. J Virol. 1990 Nov;64(11):5485–5490. doi: 10.1128/jvi.64.11.5485-5490.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Muller D. E., Siegl G. Maturation of parvovirus LuIII in a subcellular system. I. Optimal conditions for in vitro synthesis and encapsidation of viral DNA. J Gen Virol. 1983 May;64(Pt 5):1043–1054. doi: 10.1099/0022-1317-64-5-1043. [DOI] [PubMed] [Google Scholar]
  10. Parrish C. R., Aquadro C. F., Carmichael L. E. Canine host range and a specific epitope map along with variant sequences in the capsid protein gene of canine parvovirus and related feline, mink, and raccoon parvoviruses. Virology. 1988 Oct;166(2):293–307. doi: 10.1016/0042-6822(88)90500-4. [DOI] [PubMed] [Google Scholar]
  11. Pintel D., Dadachanji D., Astell C. R., Ward D. C. The genome of minute virus of mice, an autonomous parvovirus, encodes two overlapping transcription units. Nucleic Acids Res. 1983 Feb 25;11(4):1019–1038. doi: 10.1093/nar/11.4.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rhode S. L., 3rd, Klaassen B. DNA sequence of the 5' terminus containing the replication origin of parvovirus replicative form DNA. J Virol. 1982 Mar;41(3):990–999. doi: 10.1128/jvi.41.3.990-999.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rhode S. L., 3rd Replication process of the parvovirus H-1 V. Isolation and characterization of temperature-sensitive H-1 mutants. J Virol. 1976 Feb;17(2):659–667. doi: 10.1128/jvi.17.2.659-667.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Salzman L. A., White W. L. Structural proteins of Kilham rat virus. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1551–1556. doi: 10.1016/0006-291x(70)90564-4. [DOI] [PubMed] [Google Scholar]
  15. Tattersall P., Cawte P. J., Shatkin A. J., Ward D. C. Three structural polypeptides coded for by minite virus of mice, a parvovirus. J Virol. 1976 Oct;20(1):273–289. doi: 10.1128/jvi.20.1.273-289.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tullis G. E., Burger L. R., Pintel D. J. The minor capsid protein VP1 of the autonomous parvovirus minute virus of mice is dispensable for encapsidation of progeny single-stranded DNA but is required for infectivity. J Virol. 1993 Jan;67(1):131–141. doi: 10.1128/jvi.67.1.131-141.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Willwand K., Hirt B. The minute virus of mice capsid specifically recognizes the 3' hairpin structure of the viral replicative-form DNA: mapping of the binding site by hydroxyl radical footprinting. J Virol. 1991 Sep;65(9):4629–4635. doi: 10.1128/jvi.65.9.4629-4635.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Willwand K., Kaaden O. R. Capsid protein VP1 (p85) of Aleutian disease virus is a major DNA-binding protein. Virology. 1988 Sep;166(1):52–57. doi: 10.1016/0042-6822(88)90145-6. [DOI] [PubMed] [Google Scholar]
  19. Willwand K., Kaaden O. R. Proteins of viral and cellular origin bind to the Aleutian disease virus (ADV) DNA 3'-terminal hairpin: presentation of a scheme for encapsidation of ADV DNA. J Virol. 1990 Apr;64(4):1598–1605. doi: 10.1128/jvi.64.4.1598-1605.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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