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. 1993 Dec;67(12):7513–7521. doi: 10.1128/jvi.67.12.7513-7521.1993

Divergence and evolution of homologous regions of Bombyx mori nuclear polyhedrosis virus.

K Majima 1, R Kobara 1, S Maeda 1
PMCID: PMC238217  PMID: 8230471

Abstract

Homologous regions (hrs) (hr1,hr2-left,hr2-right,hr3,hr4-left,hr 4-right, and hr5) similar to those found in the Autographa californica nuclear polyhedrosis virus (AcNPV) genome were found in the Bombyx mori NPV (BmNPV) genome. The BmNPV hrs contained two to eight repeats of a homologous nucleotide sequence which were on average about 75 bp long. All of these homologous sequence repeats contained a 26-bp-long palindrome motif with an EcoRI or EcoRI-like site at its core. The consensus sequence of the BmNPV hrs showed 95% conservation with respect to those found in AcNPV. Nucleotide sequence analysis indicated that hr2-left and hr2-right of BmNPV evolved from an ancestor similar to hr2 of AcNPV by inversion, cleavage, and ligation. The polarities of the BmNPV and AcNPV hrs were conserved except for that of hr4-left. Within hr4-right of BmNPV, four repeats of a previously underscribed palindrome motif were found. Bmhr5D, a BmNPV mutant which lacked hr5, replicated at a rate similar to that of wild-type BmNPV in BmN cells and silkworm larvae, indicating that hr5 was not essential for viral replication. After ten passages of Bmhr5D in BmN cells, no detectable changes in its genome were observed by restriction endonuclease analysis. The evolution and divergence of the BmNPV genome are also discussed.

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Selected References

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  1. Blissard G. W., Rohrmann G. F. Baculovirus diversity and molecular biology. Annu Rev Entomol. 1990;35:127–155. doi: 10.1146/annurev.en.35.010190.001015. [DOI] [PubMed] [Google Scholar]
  2. Braunagel S. C., Daniel K. D., Reilly L. M., Guarino L. A., Hong T., Summers M. D. Sequence, genomic organization of the EcoRI-A fragment of Autographa californica nuclear polyhedrosis virus, and identification of a viral-encoded protein resembling the outer capsid protein VP8 of rotavirus. Virology. 1992 Dec;191(2):1003–1008. doi: 10.1016/0042-6822(92)90281-s. [DOI] [PubMed] [Google Scholar]
  3. Carson D. D., Summers M. D., Guarino L. A. Transient expression of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements. J Virol. 1991 Feb;65(2):945–951. doi: 10.1128/jvi.65.2.945-951.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen D. D., Nesson M. H., Rohrmann G. F., Beaudreau G. S. The genome of the multicapsid baculovirus of Orgyia pseudotsugata: restriction map and analysis of two sets of GC-rich repeated sequences. J Gen Virol. 1988 Jun;69(Pt 6):1375–1381. doi: 10.1099/0022-1317-69-6-1375. [DOI] [PubMed] [Google Scholar]
  5. Cochran M. A., Faulkner P. Location of Homologous DNA Sequences Interspersed at Five Regions in the Baculovirus AcMNPV Genome. J Virol. 1983 Mar;45(3):961–970. doi: 10.1128/jvi.45.3.961-970.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friesen P. D., Miller L. K. Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1987 Jul;61(7):2264–2272. doi: 10.1128/jvi.61.7.2264-2272.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guarino L. A., Dong W. Expression of an enhancer-binding protein in insect cells transfected with the Autographa californica nuclear polyhedrosis virus IE1 gene. J Virol. 1991 Jul;65(7):3676–3680. doi: 10.1128/jvi.65.7.3676-3680.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guarino L. A., Gonzalez M. A., Summers M. D. Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus. J Virol. 1986 Oct;60(1):224–229. doi: 10.1128/jvi.60.1.224-229.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guarino L. A., Summers M. D. Interspersed Homologous DNA of Autographa californica Nuclear Polyhedrosis Virus Enhances Delayed-Early Gene Expression. J Virol. 1986 Oct;60(1):215–223. doi: 10.1128/jvi.60.1.215-223.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kamita S. G., Majima K., Maeda S. Identification and characterization of the p35 gene of Bombyx mori nuclear polyhedrosis virus that prevents virus-induced apoptosis. J Virol. 1993 Jan;67(1):455–463. doi: 10.1128/jvi.67.1.455-463.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kondo A., Maeda S. Host range expansion by recombination of the baculoviruses Bombyx mori nuclear polyhedrosis virus and Autographa californica nuclear polyhedrosis virus. J Virol. 1991 Jul;65(7):3625–3632. doi: 10.1128/jvi.65.7.3625-3632.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kool M., van den Berg P. M., Tramper J., Goldbach R. W., Vlak J. M. Location of two putative origins of DNA replication of Autographa californica nuclear polyhedrosis virus. Virology. 1993 Jan;192(1):94–101. doi: 10.1006/viro.1993.1011. [DOI] [PubMed] [Google Scholar]
  13. Kumar S., Miller L. K. Effects of serial passage of Autographa californica nuclear polyhedrosis virus in cell culture. Virus Res. 1987 Jun;7(4):335–349. doi: 10.1016/0168-1702(87)90047-5. [DOI] [PubMed] [Google Scholar]
  14. Liu A., Qin J. C., Rankin C., Hardin S. E., Weaver R. F. Nucleotide sequence of a portion of the Autographa californica nuclear polyhedrosis virus genome containing the EcoRI site-rich region (hr5) and an open reading frame just 5' of the p10 gene. J Gen Virol. 1986 Nov;67(Pt 11):2565–2570. doi: 10.1099/0022-1317-67-11-2565. [DOI] [PubMed] [Google Scholar]
  15. Maeda S. Expression of foreign genes in insects using baculovirus vectors. Annu Rev Entomol. 1989;34:351–372. doi: 10.1146/annurev.en.34.010189.002031. [DOI] [PubMed] [Google Scholar]
  16. Maeda S., Kamita S. G., Kataoka H. The basic DNA-binding protein of Bombyx mori nuclear polyhedrosis virus: the existence of an additional arginine repeat. Virology. 1991 Feb;180(2):807–810. doi: 10.1016/0042-6822(91)90096-t. [DOI] [PubMed] [Google Scholar]
  17. Maeda S., Kawai T., Obinata M., Fujiwara H., Horiuchi T., Saeki Y., Sato Y., Furusawa M. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature. 1985 Jun 13;315(6020):592–594. doi: 10.1038/315592a0. [DOI] [PubMed] [Google Scholar]
  18. Maeda S., Majima K. Molecular cloning and physical mapping of the genome of Bombyx mori nuclear polyhedrosis virus. J Gen Virol. 1990 Aug;71(Pt 8):1851–1855. doi: 10.1099/0022-1317-71-8-1851. [DOI] [PubMed] [Google Scholar]
  19. Maeda S., Mukohara Y., Kondo A. Characteristically distinct isolates of the nuclear polyhedrosis virus from Spodoptera litura. J Gen Virol. 1990 Nov;71(Pt 11):2631–2639. doi: 10.1099/0022-1317-71-11-2631. [DOI] [PubMed] [Google Scholar]
  20. Miller D. W., Miller L. K. A virus mutant with an insertion of a copia-like transposable element. Nature. 1982 Oct 7;299(5883):562–564. doi: 10.1038/299562a0. [DOI] [PubMed] [Google Scholar]
  21. Miller L. K. Baculoviruses for foreign gene expression in insect cells. Biotechnology. 1988;10:457–465. doi: 10.1016/b978-0-409-90042-2.50029-5. [DOI] [PubMed] [Google Scholar]
  22. Nissen M. S., Friesen P. D. Molecular analysis of the transcriptional regulatory region of an early baculovirus gene. J Virol. 1989 Feb;63(2):493–503. doi: 10.1128/jvi.63.2.493-503.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pearson M., Bjornson R., Pearson G., Rohrmann G. The Autographa californica baculovirus genome: evidence for multiple replication origins. Science. 1992 Sep 4;257(5075):1382–1384. doi: 10.1126/science.1529337. [DOI] [PubMed] [Google Scholar]
  24. Rohrmann G. F. Baculovirus structural proteins. J Gen Virol. 1992 Apr;73(Pt 4):749–761. doi: 10.1099/0022-1317-73-4-749. [DOI] [PubMed] [Google Scholar]
  25. Russell R. L., Rohrmann G. F. Nucleotide sequence of the ubiquitin-39K gene region from the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome. J Gen Virol. 1993 Jun;74(Pt 6):1191–1195. doi: 10.1099/0022-1317-74-6-1191. [DOI] [PubMed] [Google Scholar]
  26. Theilmann D. A., Stewart S. Tandemly repeated sequence at the 3' end of the IE-2 gene of the baculovirus Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus is an enhancer element. Virology. 1992 Mar;187(1):97–106. doi: 10.1016/0042-6822(92)90298-4. [DOI] [PubMed] [Google Scholar]
  27. Vlak J. M., Schouten A., Usmany M., Belsham G. J., Klinge-Roode E. C., Maule A. J., Van Lent J. W., Zuidema D. Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method. Virology. 1990 Nov;179(1):312–320. doi: 10.1016/0042-6822(90)90299-7. [DOI] [PubMed] [Google Scholar]

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