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. 1993 Dec;67(12):7663–7667. doi: 10.1128/jvi.67.12.7663-7667.1993

Identification and characterization of an extended Tax protein in human T-cell lymphotropic virus type II subtype b isolates.

D Pardi 1, J E Kaplan 1, J E Coligan 1, T M Folks 1, R B Lal 1
PMCID: PMC238237  PMID: 8230487

Abstract

The tax gene sequence of human T-cell lymphotropic virus type II isolate G12 (HTLV-IIG12) was found to encode an extended Tax protein when compared with that of HTLV-IIMoT. In vitro transcription-translation of the HTLV-IIG12 tax gene produced a 40-kDa Tax protein that specifically reacted with serum specimens from HTLV-II-infected individuals. Limited sequence analysis demonstrated that isolates with an extended Tax protein were all HTLV-II subtype b (HTLV-IIb). Therefore, the extended Tax protein appears to be a unique characteristic of most HTLV-IIb isolates and may be useful in designing immunoassays to distinguish between HTLV-IIa and HTLV-IIb.

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Selected References

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  1. Biglione M., Gessain A., Quiruelas S., Fay O., Taborda M. A., Fernandez E., Lupo S., Panzita A., de The G. Endemic HTLV-II infection among Tobas and Matacos Amerindians from north Argentina. J Acquir Immune Defic Syndr. 1993 Jun;6(6):631–633. [PubMed] [Google Scholar]
  2. Dueńas-Barajas E., Bernal J. E., Vaught D. R., Briceño I., Durán C., Yanagihara R., Gajdusek D. C. Coexistence of human T-lymphotropic virus types I and II among the Wayuu Indians from the Guajira Region of Colombia. AIDS Res Hum Retroviruses. 1992 Nov;8(11):1851–1855. doi: 10.1089/aid.1992.8.1851. [DOI] [PubMed] [Google Scholar]
  3. Gessain A., Barin F., Vernant J. C., Gout O., Maurs L., Calender A., de Thé G. Antibodies to human T-lymphotropic virus type-I in patients with tropical spastic paraparesis. Lancet. 1985 Aug 24;2(8452):407–410. doi: 10.1016/s0140-6736(85)92734-5. [DOI] [PubMed] [Google Scholar]
  4. Hadlock K. G., Lipka J. J., Chow T. P., Foung S. K., Reyes G. R. Cloning and analysis of a recombinant antigen containing an epitope specific for human T-cell lymphotropic virus type II. Blood. 1992 May 15;79(10):2789–2796. [PubMed] [Google Scholar]
  5. Hjelle B., Appenzeller O., Mills R., Alexander S., Torrez-Martinez N., Jahnke R., Ross G. Chronic neurodegenerative disease associated with HTLV-II infection. Lancet. 1992 Mar 14;339(8794):645–646. doi: 10.1016/0140-6736(92)90797-7. [DOI] [PubMed] [Google Scholar]
  6. Hjelle B., Mills R., Swenson S., Mertz G., Key C., Allen S. Incidence of hairy cell leukemia, mycosis fungoides, and chronic lymphocytic leukemia in first known HTLV-II-endemic population. J Infect Dis. 1991 Mar;163(3):435–440. doi: 10.1093/infdis/163.3.435. [DOI] [PubMed] [Google Scholar]
  7. Horal P., Hall W. W., Svennerholm B., Lycke J., Jeansson S., Rymo L., Kaplan M. H., Vahlne A. Identification of type-specific linear epitopes in the glycoproteins gp46 and gp21 of human T-cell leukemia viruses type I and type II using synthetic peptides. Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5754–5758. doi: 10.1073/pnas.88.13.5754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jacobson S., Lehky T., Nishimura M., Robinson S., McFarlin D. E., Dhib-Jalbut S. Isolation of HTLV-II from a patient with chronic, progressive neurological disease clinically indistinguishable from HTLV-I-associated myelopathy/tropical spastic paraparesis. Ann Neurol. 1993 Apr;33(4):392–396. doi: 10.1002/ana.410330411. [DOI] [PubMed] [Google Scholar]
  9. Jacobson S., Raine C. S., Mingioli E. S., McFarlin D. E. Isolation of an HTLV-1-like retrovirus from patients with tropical spastic paraparesis. Nature. 1988 Feb 11;331(6156):540–543. doi: 10.1038/331540a0. [DOI] [PubMed] [Google Scholar]
  10. Kalyanaraman V. S., Sarngadharan M. G., Robert-Guroff M., Miyoshi I., Golde D., Gallo R. C. A new subtype of human T-cell leukemia virus (HTLV-II) associated with a T-cell variant of hairy cell leukemia. Science. 1982 Nov 5;218(4572):571–573. doi: 10.1126/science.6981847. [DOI] [PubMed] [Google Scholar]
  11. Khabbaz R. F., Hartel D., Lairmore M., Horsburgh C. R., Schoenbaum E. E., Roberts B., Hartley T. M., Friedland G. Human T lymphotropic virus type II (HTLV-II) infection in a cohort of New York intravenous drug users: an old infection? J Infect Dis. 1991 Feb;163(2):252–256. doi: 10.1093/infdis/163.2.252. [DOI] [PubMed] [Google Scholar]
  12. Khabbaz R. F., Onorato I. M., Cannon R. O., Hartley T. M., Roberts B., Hosein B., Kaplan J. E. Seroprevalence of HTLV-1 and HTLV-2 among intravenous drug users and persons in clinics for sexually transmitted diseases. N Engl J Med. 1992 Feb 6;326(6):375–380. doi: 10.1056/NEJM199202063260604. [DOI] [PubMed] [Google Scholar]
  13. Kwok S., Gallo D., Hanson C., McKinney N., Poiesz B., Sninsky J. J. High prevalence of HTLV-II among intravenous drug abusers: PCR confirmation and typing. AIDS Res Hum Retroviruses. 1990 Apr;6(4):561–565. doi: 10.1089/aid.1990.6.561. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lairmore M. D., Jacobson S., Gracia F., De B. K., Castillo L., Larreategui M., Roberts B. D., Levine P. H., Blattner W. A., Kaplan J. E. Isolation of human T-cell lymphotropic virus type 2 from Guaymi Indians in Panama. Proc Natl Acad Sci U S A. 1990 Nov;87(22):8840–8844. doi: 10.1073/pnas.87.22.8840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lal R. B., Rudolph D. L., Kaplan J. E., Hjelle B., Levine P. H., Coligan J. E., Viscidi R. P. Identification of immunodominant epitopes in envelope glycoprotein of human T lymphotropic virus type II. Virology. 1992 Jan;186(1):274–279. doi: 10.1016/0042-6822(92)90081-y. [DOI] [PubMed] [Google Scholar]
  17. Lal R. B., Rudolph D. L., Lairmore M. D., Khabbaz R. F., Garfield M., Coligan J. E., Folks T. M. Serologic discrimination of human T cell lymphotropic virus infection by using a synthetic peptide-based enzyme immunoassay. J Infect Dis. 1991 Jan;163(1):41–46. doi: 10.1093/infdis/163.1.41. [DOI] [PubMed] [Google Scholar]
  18. Lee H. H., Swanson P., Rosenblatt J. D., Chen I. S., Sherwood W. C., Smith D. E., Tegtmeier G. E., Fernando L. P., Fang C. T., Osame M. Relative prevalence and risk factors of HTLV-I and HTLV-II infection in US blood donors. Lancet. 1991 Jun 15;337(8755):1435–1439. doi: 10.1016/0140-6736(91)93126-t. [DOI] [PubMed] [Google Scholar]
  19. Lee H., Swanson P., Shorty V. S., Zack J. A., Rosenblatt J. D., Chen I. S. High rate of HTLV-II infection in seropositive i.v. drug abusers in New Orleans. Science. 1989 Apr 28;244(4903):471–475. doi: 10.1126/science.2655084. [DOI] [PubMed] [Google Scholar]
  20. Levine P. H., Jacobson S., Elliott R., Cavallero A., Colclough G., Dorry C., Stephenson C., Knigge R. M., Drummond J., Nishimura M. HTLV-II infection in Florida Indians. AIDS Res Hum Retroviruses. 1993 Feb;9(2):123–127. doi: 10.1089/aid.1993.9.123. [DOI] [PubMed] [Google Scholar]
  21. Maloney E. M., Biggar R. J., Neel J. V., Taylor M. E., Hahn B. H., Shaw G. M., Blattner W. A. Endemic human T cell lymphotropic virus type II infection among isolated Brazilian Amerindians. J Infect Dis. 1992 Jul;166(1):100–107. doi: 10.1093/infdis/166.1.100. [DOI] [PubMed] [Google Scholar]
  22. Pardi D., Switzer W. M., Hadlock K. G., Kaplan J. E., Lal R. B., Folks T. M. Complete nucleotide sequence of an Amerindian human T-cell lymphotropic virus type II (HTLV-II) isolate: identification of a variant HTLV-II subtype b from a Guaymi Indian. J Virol. 1993 Aug;67(8):4659–4664. doi: 10.1128/jvi.67.8.4659-4664.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415–7419. doi: 10.1073/pnas.77.12.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rosenblatt J. D., Golde D. W., Wachsman W., Giorgi J. V., Jacobs A., Schmidt G. M., Quan S., Gasson J. C., Chen I. S. A second isolate of HTLV-II associated with atypical hairy-cell leukemia. N Engl J Med. 1986 Aug 7;315(6):372–377. doi: 10.1056/NEJM198608073150606. [DOI] [PubMed] [Google Scholar]
  25. Shimotohno K., Takahashi Y., Shimizu N., Gojobori T., Golde D. W., Chen I. S., Miwa M., Sugimura T. Complete nucleotide sequence of an infectious clone of human T-cell leukemia virus type II: an open reading frame for the protease gene. Proc Natl Acad Sci U S A. 1985 May;82(10):3101–3105. doi: 10.1073/pnas.82.10.3101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tosswill J. H., Parry J. V., Weber J. N. Application of screening and confirmatory assays for anti-HTLV-I/II in U.K. populations. J Med Virol. 1992 Mar;36(3):167–171. doi: 10.1002/jmv.1890360304. [DOI] [PubMed] [Google Scholar]
  27. Wiktor S. Z., Cannon R. O., Atkinson W. L., Lutz B., Hook E. W., 3rd, Blattner W. A., Quinn T. C. Infection with human T lymphotropic virus types I and II in sexually transmitted disease clinics in Baltimore and New Orleans. J Infect Dis. 1992 May;165(5):920–924. doi: 10.1093/infdis/165.5.920. [DOI] [PubMed] [Google Scholar]
  28. Wong-Staal F., Gallo R. C. Human T-lymphotropic retroviruses. Nature. 1985 Oct 3;317(6036):395–403. doi: 10.1038/317395a0. [DOI] [PubMed] [Google Scholar]
  29. Yoshida M., Seiki M., Yamaguchi K., Takatsuki K. Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in the disease. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2534–2537. doi: 10.1073/pnas.81.8.2534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zella D., Mori L., Sala M., Ferrante P., Casoli C., Magnani G., Achilli G., Cattaneo E., Lori F., Bertazzoni U. HTLV-II infection in Italian drug abusers. Lancet. 1990 Sep 1;336(8714):575–576. doi: 10.1016/0140-6736(90)92140-d. [DOI] [PubMed] [Google Scholar]

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