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. 1993 Dec;67(12):7680–7683. doi: 10.1128/jvi.67.12.7680-7683.1993

Identification of a lytic-phase origin of DNA replication in human herpesvirus 6B strain Z29.

S Dewhurst 1, S C Dollard 1, P E Pellett 1, T R Dambaugh 1
PMCID: PMC238241  PMID: 8230490

Abstract

DNA sequences which have structural features suggestive of their functioning as an origin of lytic-phase DNA replication were previously identified in both human herpesvirus 6B strain Z29 [HHV-6B (Z29)] and in HHV-6A (U1102). Plasmid constructs containing the putative HHV-6B (Z29) oriLyt element were replicated after transfection into permissive T cells, when trans-acting factors were provided by HHV-6B (R-1) infection. By using this assay, the HHV-6B (Z29) oriLyt was mapped to a minimal region of approximately 400 bp which lies upstream of the gene that is homologous to herpes simplex virus UL29, a region that carries an origin in other betaherpesviruses and in some alphaherpesviruses.

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Selected References

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