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. 1992 Jan;66(1):399–404. doi: 10.1128/jvi.66.1.399-404.1992

Influenza A virus transfectants with chimeric hemagglutinins containing epitopes from different subtypes.

S Q Li 1, J L Schulman 1, T Moran 1, C Bona 1, P Palese 1
PMCID: PMC238299  PMID: 1370088

Abstract

Influenza virus transfectants with chimeric hemagglutinins were constructed by using a ribonucleoprotein transfection method. Transfectants W(H1)-H2 and W(H1)-H3 contained A/WSN/33(H1N1) (WSN) hemagglutinins in which the six-amino-acid loop (contained in antigenic site B) was replaced by the corresponding structures of influenza viruses A/Japan/57(H2N2) and A/Hong Kong/8/68(H3N2) (HK), respectively. Serological analysis indicated that the W(H1)-H3 transfectant virus reacted with antibodies against both the WSN and HK viruses in hemagglutination inhibition and plaque neutralization assays. Furthermore, mice immunized with W(H1)-H3 transfectant virus produced antibodies to the WSN and HK viruses. The results demonstrate that influenza virus transfectants can be engineered to express epitopes of different subtypes on their hemagglutinins.

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Selected References

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