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. 1992 Jan;66(1):591–595. doi: 10.1128/jvi.66.1.591-595.1992

p53 shares an antigenic determinant with proteins of 92 and 150 kilodaltons that may be involved in senescence of human cells.

S J van den Heuvel 1, S I The 1, B Klein 1, A G Jochemsen 1, A Zantema 1, A J van der Eb 1
PMCID: PMC238322  PMID: 1370093

Abstract

A panel of primary human cells and virus-transformed derivatives were tested for events that coincide with immortalization. In all primary and precrisis cells, two proteins of 92 and 150 kDa that shared an epitope with p53 were found; in most of their immortalized derivatives, however, they were absent. Expression of these proteins may be involved in senescence.

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Selected References

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  1. Arai N., Nomura D., Yokota K., Wolf D., Brill E., Shohat O., Rotter V. Immunologically distinct p53 molecules generated by alternative splicing. Mol Cell Biol. 1986 Sep;6(9):3232–3239. doi: 10.1128/mcb.6.9.3232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ball R. K., Siegl B., Quellhorst S., Brandner G., Braun D. G. Monoclonal antibodies against simian virus 40 nuclear large T tumour antigen: epitope mapping, papova virus cross-reaction and cell surface staining. EMBO J. 1984 Jul;3(7):1485–1491. doi: 10.1002/j.1460-2075.1984.tb02000.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Banks L., Matlashewski G., Crawford L. Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression. Eur J Biochem. 1986 Sep 15;159(3):529–534. doi: 10.1111/j.1432-1033.1986.tb09919.x. [DOI] [PubMed] [Google Scholar]
  4. Chang S. E. In vitro transformation of human epithelial cells. Biochim Biophys Acta. 1986;823(3):161–194. doi: 10.1016/0304-419x(86)90001-6. [DOI] [PubMed] [Google Scholar]
  5. Crawford L. V., Pim D. C., Gurney E. G., Goodfellow P., Taylor-Papadimitriou J. Detection of a common feature in several human tumor cell lines--a 53,000-dalton protein. Proc Natl Acad Sci U S A. 1981 Jan;78(1):41–45. doi: 10.1073/pnas.78.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gallimore P. H., Grand R. J., Byrd P. J. Transformation of human embryo retinoblasts with simian virus 40, adenovirus and ras oncogenes. Anticancer Res. 1986 May-Jun;6(3 Pt B):499–508. [PubMed] [Google Scholar]
  7. Gannon J. V., Lane D. P. p53 and DNA polymerase alpha compete for binding to SV40 T antigen. Nature. 1987 Oct 1;329(6138):456–458. doi: 10.1038/329456a0. [DOI] [PubMed] [Google Scholar]
  8. Gurney E. G., Harrison R. O., Fenno J. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol. 1980 Jun;34(3):752–763. doi: 10.1128/jvi.34.3.752-763.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harlow E., Whyte P., Franza B. R., Jr, Schley C. Association of adenovirus early-region 1A proteins with cellular polypeptides. Mol Cell Biol. 1986 May;6(5):1579–1589. doi: 10.1128/mcb.6.5.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Harris N., Brill E., Shohat O., Prokocimer M., Wolf D., Arai N., Rotter V. Molecular basis for heterogeneity of the human p53 protein. Mol Cell Biol. 1986 Dec;6(12):4650–4656. doi: 10.1128/mcb.6.12.4650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kano Y., Little J. B. Efficient immortalization by SV40 T DNA of skin fibroblasts from patients with Wilms' tumor associated with chromosome 11p deletion. Mol Carcinog. 1989;2(6):314–321. doi: 10.1002/mc.2940020606. [DOI] [PubMed] [Google Scholar]
  12. Klein B., Pastink A., Odijk H., Westerveld A., van der Eb A. J. Transformation and immortalization of diploid xeroderma pigmentosum fibroblasts. Exp Cell Res. 1990 Dec;191(2):256–262. doi: 10.1016/0014-4827(90)90012-y. [DOI] [PubMed] [Google Scholar]
  13. Levine A. J., Momand J. Tumor suppressor genes: the p53 and retinoblastoma sensitivity genes and gene products. Biochim Biophys Acta. 1990 Jun 1;1032(1):119–136. doi: 10.1016/0304-419x(90)90015-s. [DOI] [PubMed] [Google Scholar]
  14. Oren M. The p53 cellular tumor antigen: gene structure, expression and protein properties. Biochim Biophys Acta. 1985 Nov 12;823(1):67–78. doi: 10.1016/0304-419x(85)90015-0. [DOI] [PubMed] [Google Scholar]
  15. Smith J. R., Pereira-Smith O. M. Genetic and molecular studies of cellular immortalization. Adv Cancer Res. 1990;54:63–77. doi: 10.1016/s0065-230x(08)60808-8. [DOI] [PubMed] [Google Scholar]
  16. Stein G. H., Beeson M., Gordon L. Failure to phosphorylate the retinoblastoma gene product in senescent human fibroblasts. Science. 1990 Aug 10;249(4969):666–669. doi: 10.1126/science.2166342. [DOI] [PubMed] [Google Scholar]
  17. Thömmes P., Reiter T., Knippers R. Synthesis of DNA polymerase alpha analyzed by immunoprecipitation from synchronously proliferating cells. Biochemistry. 1986 Mar 25;25(6):1308–1314. doi: 10.1021/bi00354a018. [DOI] [PubMed] [Google Scholar]
  18. Vandekerckhove J., Bauw G., Vancompernolle K., Honoré B., Celis J. Comparative two-dimensional gel analysis and microsequencing identifies gelsolin as one of the most prominent downregulated markers of transformed human fibroblast and epithelial cells. J Cell Biol. 1990 Jul;111(1):95–102. doi: 10.1083/jcb.111.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wade-Evans A., Jenkins J. R. Precise epitope mapping of the murine transformation-associated protein, p53. EMBO J. 1985 Mar;4(3):699–706. doi: 10.1002/j.1460-2075.1985.tb03686.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Whittaker J. L., Byrd P. J., Grand R. J., Gallimore P. H. Isolation and characterization of four adenovirus type 12-transformed human embryo kidney cell lines. Mol Cell Biol. 1984 Jan;4(1):110–116. doi: 10.1128/mcb.4.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van den Heuvel S. J., van Laar T., Kast W. M., Melief C. J., Zantema A., van der Eb A. J. Association between the cellular p53 and the adenovirus 5 E1B-55kd proteins reduces the oncogenicity of Ad-transformed cells. EMBO J. 1990 Aug;9(8):2621–2629. doi: 10.1002/j.1460-2075.1990.tb07444.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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