Abstract
An axenic culture of a polyvinyl alcohol (PVA)-degrading symbiont, Pseudomonas sp. strain VM15C, was established on PVA with a crude preparation of the growth factor (factor A) produced by the symbiotic partner Pseudomonas putida VM15A. An increase of factor A in the culture medium enhanced the cell-associated PVA oxidase activity as well as the growth rate, but decreased production of extracellular PVA oxidase. PVA oxidase in cells grown on PVA was present in the periplasmic space at a higher ratio than in cells grown on peptone. PVA degradation occurred rapidly with washed cells. PVA was also degraded by immobilized cells entrapped in agar gels.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anraku Y., Heppel L. A. On the nature of the changes induced in Escherichia coli by osmotic shock. J Biol Chem. 1967 May 25;242(10):2561–2569. [PubMed] [Google Scholar]
- Sakazawa C., Shimao M., Taniguchi Y., Kato N. Symbiotic utilization of polyvinyl alcohol by mixed cultures. Appl Environ Microbiol. 1981 Jan;41(1):261–267. doi: 10.1128/aem.41.1.261-267.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimao M., Saimoto H., Kato N., Sakazawa C. Properties and roles of bacterial symbionts of polyvinyl alcohol-utilizing mixed cultures. Appl Environ Microbiol. 1983 Sep;46(3):605–610. doi: 10.1128/aem.46.3.605-610.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimao M., Taniguchi Y., Shikata S., Kato N., Sakazawa C. Production of polyvinyl alcohol oxidase by a symbiotic mixed culture. Appl Environ Microbiol. 1982 Jul;44(1):28–32. doi: 10.1128/aem.44.1.28-32.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watanabe Y., Hamada N., Morita M., Tsujisaka Y. Purification and properties of a polyvinyl alcohol-degrading enzyme produced by a strain of Pseudomonas. Arch Biochem Biophys. 1976 Jun;174(2):575–581. doi: 10.1016/0003-9861(76)90386-6. [DOI] [PubMed] [Google Scholar]