Abstract
Batch and fed-batch experiments were conducted to examine the kinetics of pentachlorophenol utilization by an enrichment culture of pentachlorophenol-degrading bacteria. The Haldane modification of the Monod equation was found to describe the relationship between the specific growth rate and substrate concentration. Analysis of the kinetic parameters indicated that the maximum specific growth rate and yield coefficients are low, with values of 0.074 h-1 and 0.136 g/g, respectively. The Monod constant (Ks) was estimated to be 60 micrograms/liter, indicating a high affinity of the microorganisms for the substrate. However, high concentrations (KI = 1,375 micrograms/liter) were shown to be inhibitory for metabolism and growth. These kinetic parameters can be used to define the optimal conditions for the removal of pentachlorophenol in biological treatment systems.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boyle T. P., Robinson-Wilson E. F., Petty J. D., Weber W. Degradation of pentachlorophenol in simulated lentic environments. Bull Environ Contam Toxicol. 1980 Feb;24(2):177–184. doi: 10.1007/BF01608094. [DOI] [PubMed] [Google Scholar]
- Chu J. P., Kirsch E. J. Metabolism of pentachlorophenol by an axenic bacterial culture. Appl Microbiol. 1972 May;23(5):1033–1035. doi: 10.1128/am.23.5.1033-1035.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edwards V. H. The influence of high substrate concentrations on microbial kinetics. Biotechnol Bioeng. 1970 Sep;12(5):679–712. doi: 10.1002/bit.260120504. [DOI] [PubMed] [Google Scholar]
- Kirsch E. J., Etzel J. E. Microbial decomposition of pentachlorophenol. J Water Pollut Control Fed. 1973 Feb;45(2):359–364. [PubMed] [Google Scholar]
- Murthy N. B., Kaufman D. D., Fries G. F. Degradation of pentachlorophenol (PCP) in aerobic and anaerobic soil. J Environ Sci Health B. 1979;14(1):1–14. doi: 10.1080/03601237909372110. [DOI] [PubMed] [Google Scholar]
- Pignatello J. J., Martinson M. M., Steiert J. G., Carlson R. E., Crawford R. L. Biodegradation and photolysis of pentachlorophenol in artificial freshwater streams. Appl Environ Microbiol. 1983 Nov;46(5):1024–1031. doi: 10.1128/aem.46.5.1024-1031.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson J. A., Tiedje J. M. Nonlinear estimation of Monod growth kinetic parameters from a single substrate depletion curve. Appl Environ Microbiol. 1983 May;45(5):1453–1458. doi: 10.1128/aem.45.5.1453-1458.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanlake G. J., Finn R. K. Isolation and characterization of a pentachlorophenol-degrading bacterium. Appl Environ Microbiol. 1982 Dec;44(6):1421–1427. doi: 10.1128/aem.44.6.1421-1427.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yang R. D., Humphrey A. E. Dynamic and steady state studies of phenol biodegradation in pure and mixed cultures. Biotechnol Bioeng. 1975 Aug;17(8):1211–1235. doi: 10.1002/bit.260170809. [DOI] [PubMed] [Google Scholar]