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. 1985 Jan;49(1):168–172. doi: 10.1128/aem.49.1.168-172.1985

Preparation and characterization of monoclonal antibodies to the trichothecene mycotoxin T-2.

K W Hunter Jr, A A Brimfield, M Miller, F D Finkelman, S F Chu
PMCID: PMC238364  PMID: 3919640

Abstract

Two mouse immunoglobulin G1 monoclonal antibodies that bind to the trichothecene mycotoxin T-2 were prepared. These antibodies, designated 12C12 and 15H6, had affinities for T-2 of 3.5 X 10(6) and 5.8 X 10(7) liters/mol, respectively. A competitive inhibition enzyme immunoassay that employed these antibodies had a sensitivity for T-2 of 50 ng per assay. Both antibodies bound to the metabolite HT-2 but not to the related trichothecenes monoacetoxyscirpenol, diacetoxyscirpenol, deoxynivalenol, and deoxyverrucarol. Evidence is presented that T-2-protein conjugates inhibit protein synthesis in lymphoid cells and that this apparent immunotoxicity may be due to the release of T-2 from the protein carrier.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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