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. 1985 Jan;49(1):247–249. doi: 10.1128/aem.49.1.247-249.1985

Production and Consumption of H2 during Growth of Methanosarcina spp. on Acetate

Derek R Lovley 1,†,*, James G Ferry 1
PMCID: PMC238382  PMID: 16346703

Abstract

Methanosarcina sp. strain TM-1 and Methanosarcina acetivorans produced and consumed H2 to maintain H2 partial pressures of 16 to 92 Pa in closed cultures during growth on acetate. Strain TM-1 produced H2 continuously when H2 was continuously removed from the culture. The potential physiological significance of H2 in acetate metabolism to methane is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baresi L., Wolfe R. S. Levels of coenzyme F420, coenzyme M, hydrogenase, and methylcoenzyme M methylreductase in acetate-grown Methanosarcina. Appl Environ Microbiol. 1981 Feb;41(2):388–391. doi: 10.1128/aem.41.2.388-391.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Lovley D. R., Dwyer D. F., Klug M. J. Kinetic analysis of competition between sulfate reducers and methanogens for hydrogen in sediments. Appl Environ Microbiol. 1982 Jun;43(6):1373–1379. doi: 10.1128/aem.43.6.1373-1379.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Lovley D. R., Greening R. C., Ferry J. G. Rapidly growing rumen methanogenic organism that synthesizes coenzyme M and has a high affinity for formate. Appl Environ Microbiol. 1984 Jul;48(1):81–87. doi: 10.1128/aem.48.1.81-87.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lovley D. R., White R. H., Ferry J. G. Identification of methyl coenzyme M as an intermediate in methanogenesis from acetate in Methanosarcina spp. J Bacteriol. 1984 Nov;160(2):521–525. doi: 10.1128/jb.160.2.521-525.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nelson M. J., Ferry J. G. Carbon monoxide-dependent methyl coenzyme M methylreductase in acetotrophic Methosarcina spp. J Bacteriol. 1984 Nov;160(2):526–532. doi: 10.1128/jb.160.2.526-532.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. O'Brien J. M., Wolkin R. H., Moench T. T., Morgan J. B., Zeikus J. G. Association of hydrogen metabolism with unitrophic or mixotrophic growth of Methanosarcina barkeri on carbon monoxide. J Bacteriol. 1984 Apr;158(1):373–375. doi: 10.1128/jb.158.1.373-375.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Robinson J. A., Tiedje J. M. Kinetics of hydrogen consumption by rumen fluid, anaerobic digestor sludge, and sediment. Appl Environ Microbiol. 1982 Dec;44(6):1374–1384. doi: 10.1128/aem.44.6.1374-1384.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schauer N. L., Ferry J. G. Metabolism of formate in Methanobacterium formicicum. J Bacteriol. 1980 Jun;142(3):800–807. doi: 10.1128/jb.142.3.800-807.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sowers K. R., Baron S. F., Ferry J. G. Methanosarcina acetivorans sp. nov., an Acetotrophic Methane-Producing Bacterium Isolated from Marine Sediments. Appl Environ Microbiol. 1984 May;47(5):971–978. doi: 10.1128/aem.47.5.971-978.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sowers K. R., Ferry J. G. Isolation and Characterization of a Methylotrophic Marine Methanogen, Methanococcoides methylutens gen. nov., sp. nov. Appl Environ Microbiol. 1983 Feb;45(2):684–690. doi: 10.1128/aem.45.2.684-690.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weimer P. J., Zeikus J. G. Acetate metabolism in Methanosarcina barkeri. Arch Microbiol. 1978 Nov 13;119(2):175–182. doi: 10.1007/BF00964270. [DOI] [PubMed] [Google Scholar]
  12. Zehnder A. J., Huser B. A., Brock T. D., Wuhrmann K. Characterization of an acetate-decarboxylating, non-hydrogen-oxidizing methane bacterium. Arch Microbiol. 1980 Jan;124(1):1–11. doi: 10.1007/BF00407022. [DOI] [PubMed] [Google Scholar]
  13. Zeikus J. G. Metabolism of one-carbon compounds by chemotrophic anaerobes. Adv Microb Physiol. 1983;24:215–299. doi: 10.1016/s0065-2911(08)60387-2. [DOI] [PubMed] [Google Scholar]
  14. Zinder S. H., Mah R. A. Isolation and Characterization of a Thermophilic Strain of Methanosarcina Unable to Use H(2)-CO(2) for Methanogenesis. Appl Environ Microbiol. 1979 Nov;38(5):996–1008. doi: 10.1128/aem.38.5.996-1008.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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