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. 1985 Jan;49(1):253–255. doi: 10.1128/aem.49.1.253-255.1985

Cytochemical Localization of Urease in a Rumen Staphylococcus sp. by Electron Microscopy

R J C McLean 3, K-J Cheng 3,*, W D Gould 3, J W Costerton 3
PMCID: PMC238384  PMID: 16346704

Abstract

We describe a technique whereby intracellular urease activity can be localized by transmission electron microscopy. The ammonia produced from the enzymatic hydrolysis of urea is first precipitated with sodium tetraphenylboron and then replaced with silver to produce electron-dense silver tetraphenylboron. This direct reaction product deposition procedure was used to demonstrate the presence of membrane-bound urease of Staphylococcus sp. H3-22, a gram-positive ruminal bacterium.

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Selected References

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