Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1985 Feb;49(2):305–309. doi: 10.1128/aem.49.2.305-309.1985

Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower.

G W Gorman, J C Feeley, A Steigerwalt, P H Edelstein, C W Moss, D J Brenner
PMCID: PMC238398  PMID: 3985609

Abstract

Between March 1980 and June 1981, five strains of Legionella-like organisms were isolated from water. Four were recovered from potable water collected from hospitals in Chicago, Ill., and Los Angeles, Calif., during outbreaks of nosocomial legionellosis. The fifth strain was isolated from water collected from an industrial cooling tower in Jamestown, N.Y. The strains exhibited biochemical reactions typical of Legionella species and were gram-negative motile rods which grew on buffered charcoal-yeast extract agar but not on blood agar, required cysteine, and were catalase positive, urease negative, nitrate negative, hippurate negative, and nonfermentative. All strains were positive for oxidase and beta-lactamase and produced a brown, diffusible pigment. Of the five strains, four exhibited blue-white autofluorescence under long-wavelength UV light. The fatty-acid composition and ubiquinone content of these strains were consistent with those of other Legionella species. Direct fluorescent-antibody examination of the five strains with conjugates to previously described Legionella species demonstrated no cross-reactions except with the conjugates to L. longbeachae serogroup 2 and L. bozemanii serogroup 2. Four strains gave a 4+ reaction to the L. longbeachae serogroup 2 conjugate and the fifth strain gave a 1+ reaction. Each of the five strains gave a 4+ reaction with the conjugate to L. bozemanii serogroup 2. DNAs from the five strains were highly related (84 to 99%) and showed 5 to 57% relatedness to other Legionella species. These strains constitute a new species in the genus Legionella, and the name Legionella anisa sp. nov. is proposed. The type strain of L. anisa is WA-316-C3 (ATCC 35292).

Full text

PDF
305

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe K., Ishibashi K., Ohmae M., Kawabe K., Katsui G. Determination of ubiquinone in serum and liver by high-speed liquid chromatography. J Nutr Sci Vitaminol (Tokyo) 1978;24(6):555–567. doi: 10.3177/jnsv.24.555. [DOI] [PubMed] [Google Scholar]
  2. Bibb W. F., Sorg R. J., Thomason B. M., Hicklin M. D., Steigerwalt A. G., Brenner D. J., Wulf M. R. Recognition of a second serogroup of Legionella longbeachae. J Clin Microbiol. 1981 Dec;14(6):674–677. doi: 10.1128/jcm.14.6.674-677.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bopp C. A., Sumner J. W., Morris G. K., Wells J. G. Isolation of Legionella spp. from environmental water samples by low-pH treatment and use of a selective medium. J Clin Microbiol. 1981 Apr;13(4):714–719. doi: 10.1128/jcm.13.4.714-719.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brenner D. J., Steigerwalt A. G., McDade J. E. Classification of the Legionnaires' disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann Intern Med. 1979 Apr;90(4):656–658. doi: 10.7326/0003-4819-90-4-656. [DOI] [PubMed] [Google Scholar]
  5. Campbell J., Bibb W. F., Lambert M. A., Eng S., Steigerwalt A. G., Allard J., Moss C. W., Brenner D. J. Legionella sainthelensi: a new species of Legionella isolated from water near Mt. St. Helens. Appl Environ Microbiol. 1984 Feb;47(2):369–373. doi: 10.1128/aem.47.2.369-373.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cherry W. B., Gorman G. W., Orrison L. H., Moss C. W., Steigerwalt A. G., Wilkinson H. W., Johnson S. E., McKinney R. M., Brenner D. J. Legionella jordanis: a new species of Legionella isolated from water and sewage. J Clin Microbiol. 1982 Feb;15(2):290–297. doi: 10.1128/jcm.15.2.290-297.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Collins M. D., Jones D. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev. 1981 Jun;45(2):316–354. doi: 10.1128/mr.45.2.316-354.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Edelstein P. H., Brenner D. J., Moss C. W., Steigerwalt A. G., Francis E. M., George W. L. Legionella wadsworthii species nova: a cause of human pneumonia. Ann Intern Med. 1982 Dec;97(6):809–813. doi: 10.7326/0003-4819-97-6-809. [DOI] [PubMed] [Google Scholar]
  9. Edelstein P. H. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol. 1981 Sep;14(3):298–303. doi: 10.1128/jcm.14.3.298-303.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feeley J. C., Gibson R. J., Gorman G. W., Langford N. C., Rasheed J. K., Mackel D. C., Baine W. B. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J Clin Microbiol. 1979 Oct;10(4):437–441. doi: 10.1128/jcm.10.4.437-441.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Herwaldt L. A., Gorman G. W., McGrath T., Toma S., Brake B., Hightower A. W., Jones J., Reingold A. L., Boxer P. A., Tang P. W. A new Legionella species, Legionella feeleii species nova, causes Pontiac fever in an automobile plant. Ann Intern Med. 1984 Mar;100(3):333–338. doi: 10.7326/0003-4819-100-3-333. [DOI] [PubMed] [Google Scholar]
  12. Karr D. E., Bibb W. F., Moss C. W. Isoprenoid quinones of the genus Legionella. J Clin Microbiol. 1982 Jun;15(6):1044–1048. doi: 10.1128/jcm.15.6.1044-1048.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McKinney R. M., Porschen R. K., Edelstein P. H., Bissett M. L., Harris P. P., Bondell S. P., Steigerwalt A. G., Weaver R. E., Ein M. E., Lindquist D. S. Legionella longbeachae species nova, another etiologic agent of human pneumonia. Ann Intern Med. 1981 Jun;94(6):739–743. doi: 10.7326/0003-4819-94-6-739. [DOI] [PubMed] [Google Scholar]
  14. Morris G. K., Steigerwalt A., Feeley J. C., Wong E. S., Martin W. T., Patton C. M., Brenner D. J. Legionella gormanii sp. nov. J Clin Microbiol. 1980 Nov;12(5):718–721. doi: 10.1128/jcm.12.5.718-721.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Moss C. W., Bibb W. F., Karr D. E., Guerrant G. O., Lambert M. A. Cellular fatty acid composition and ubiquinone content of Legionella feeleii sp. nov. J Clin Microbiol. 1983 Oct;18(4):917–919. doi: 10.1128/jcm.18.4.917-919.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Moss C. W. Gas-liquid chromatography as an analytical tool in microbiology. J Chromatogr. 1981 Jan 9;203:337–347. doi: 10.1016/s0021-9673(00)80305-2. [DOI] [PubMed] [Google Scholar]
  17. Moss C. W., Guerrant G. O. Separation of bacterial ubiquinones by reverse-phase high-pressure liquid chromatography. J Clin Microbiol. 1983 Jul;18(1):15–17. doi: 10.1128/jcm.18.1.15-17.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moss C. W., Karr D. E., Dees S. B. Cellular fatty acid composition of Legionella longbeachae sp. nov. J Clin Microbiol. 1981 Dec;14(6):692–694. doi: 10.1128/jcm.14.6.692-694.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moss C. W., Weaver R. E., Dees S. B., Cherry W. B. Cellular fatty acid composition of isolates from Legionnaires disease. J Clin Microbiol. 1977 Aug;6(2):140–143. doi: 10.1128/jcm.6.2.140-143.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Orrison L. H., Cherry W. B., Tyndall R. L., Fliermans C. B., Gough S. B., Lambert M. A., McDougal L. K., Bibb W. F., Brenner D. J. Legionella oakridgensis: unusual new species isolated from cooling tower water. Appl Environ Microbiol. 1983 Feb;45(2):536–545. doi: 10.1128/aem.45.2.536-545.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pasculle A. W., Feeley J. C., Gibson R. J., Cordes L. G., Myerowitz R. L., Patton C. M., Gorman G. W., Carmack C. L., Ezzell J. W., Dowling J. N. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J Infect Dis. 1980 Jun;141(6):727–732. doi: 10.1093/infdis/141.6.727. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES