Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1985 Feb;49(2):359–364. doi: 10.1128/aem.49.2.359-364.1985

Virion conformational forms and the complex inactivation kinetics of echovirus by chlorine in water.

D C Young, D G Sharp
PMCID: PMC238407  PMID: 2984991

Abstract

Aberrant inactivation kinetics were observed when monodispersed echovirus type 1 (Farouk) was inactivated with chlorine. An initial 1- to 2-log10-unit decrease in titer was followed by lag period, during which the titer stayed the same or increased, and this was followed by a final decline in titer. First-order kinetics were obtained with poliovirus type 1 under the same conditions. Isoelectric focusing studies of echovirus before chlorine treatment showed that the virus distributed into two pH-dependent and interconvertible isoelectric forms. After chlorine treatment all remaining virus infectivity was associated with a third pH-independent isoelectric form. The complex inactivation kinetics appeared to be due to shifts between these conformational forms during inactivation in certain ionic environments. Under certain conditions the conformational shifts resulted in substantial resistance of monodispersed echovirus to chlorine.

Full text

PDF
359

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Floyd R., Johnson J. D., Sharp D. G. Inactivation by bromine of single poliovirus particles in water. Appl Environ Microbiol. 1976 Feb;31(2):298–303. doi: 10.1128/aem.31.2.298-303.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Floyd R., Sharp D. G. Aggregation of poliovirus and reovirus by dilution in water. Appl Environ Microbiol. 1977 Jan;33(1):159–167. doi: 10.1128/aem.33.1.159-167.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Floyd R., Sharp D. G. Viral aggregation: effects of salts on the aggregation of poliovirus and reovirus at low pH. Appl Environ Microbiol. 1978 Jun;35(6):1084–1094. doi: 10.1128/aem.35.6.1084-1094.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Floyd R., Sharp D. G. Viral aggregation: quantitation and kinetics of the aggregation of poliovirus and reovirus. Appl Environ Microbiol. 1978 Jun;35(6):1079–1083. doi: 10.1128/aem.35.6.1079-1083.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jensen H., Thomas K., Sharp D. G. Inactivation of coxsackieviruses B3 and B5 in water by chlorine. Appl Environ Microbiol. 1980 Sep;40(3):633–640. doi: 10.1128/aem.40.3.633-640.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mandel B. Characterization of type 1 poliovirus by electrophoretic analysis. Virology. 1971 Jun;44(3):554–568. doi: 10.1016/0042-6822(71)90369-2. [DOI] [PubMed] [Google Scholar]
  7. Sharp D. G., Floyd R., Johnson J. D. Initial fast reaction of bromine on reovirus in turbulent flowing water. Appl Environ Microbiol. 1976 Feb;31(2):173–181. doi: 10.1128/aem.31.2.173-181.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sharp D. G., Floyd R., Johnson J. D. Nature of the surviving plaque-forming unit of reovirus in water containing bromine. Appl Microbiol. 1975 Jan;29(1):94–101. doi: 10.1128/am.29.1.94-101.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sharp D. G., Leong J. Inactivation of poliovirus I (Brunhilde) single particles by chlorine in water. Appl Environ Microbiol. 1980 Aug;40(2):381–385. doi: 10.1128/aem.40.2.381-385.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sharp D. G., Young D. C., Floyd R., Johnson J. D. Effect of ionic environment on the inactivation of poliovirus in water by chlorine. Appl Environ Microbiol. 1980 Mar;39(3):530–534. doi: 10.1128/aem.39.3.530-534.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Young D. C., Johnson J. D., Sharp D. G. The complex reaction kinetics of ECHO-1 virus with chlorine in water. Proc Soc Exp Biol Med. 1977 Dec;156(3):496–499. doi: 10.3181/00379727-156-39965. [DOI] [PubMed] [Google Scholar]
  12. Young D. C., Sharp D. G. Partial reactivation of chlorine-treated echovirus. Appl Environ Microbiol. 1979 Apr;37(4):766–773. doi: 10.1128/aem.37.4.766-773.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Young D. C., Sharp D. G. Poliovirus aggregates and their survival in water. Appl Environ Microbiol. 1977 Jan;33(1):168–177. doi: 10.1128/aem.33.1.168-177.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES