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. 1985 Apr;49(4):852–857. doi: 10.1128/aem.49.4.852-857.1985

Application of flow cytometry to detection and characterization of Legionella spp.

R L Tyndall, R E Hand Jr, R C Mann, C Evans, R Jernigan
PMCID: PMC238457  PMID: 3890740

Abstract

Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps related to infectivity, in Legionella populations.

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Selected References

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  1. Cherry W. B., Pittman B., Harris P. P., Hebert G. A., Thomason B. M., Thacker L., Weaver R. E. Detection of Legionnaires disease bacteria by direct immunofluorescent staining. J Clin Microbiol. 1978 Sep;8(3):329–338. doi: 10.1128/jcm.8.3.329-338.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cordes L. G., Fraser D. W., Skaliy P., Perlino C. A., Elsea W. R., Mallison G. F., Hayes P. S. Legionnaires' disease outbreak at an Atlanta, Georgia, Country Club: evidence for spread from an evaporative condenser. Am J Epidemiol. 1980 Apr;111(4):425–431. doi: 10.1093/oxfordjournals.aje.a112917. [DOI] [PubMed] [Google Scholar]
  3. Dondero T. J., Jr, Rendtorff R. C., Mallison G. F., Weeks R. M., Levy J. S., Wong E. W., Schaffner W. An outbreak of Legionnaires' disease associated with a contaminated air-conditioning cooling tower. N Engl J Med. 1980 Feb 14;302(7):365–370. doi: 10.1056/NEJM198002143020703. [DOI] [PubMed] [Google Scholar]
  4. Feeley J. C., Gorman G. W., Weaver R. E., Mackel D. C., Smith H. W. Primary isolation media for Legionnaires disease bacterium. J Clin Microbiol. 1978 Sep;8(3):320–325. doi: 10.1128/jcm.8.3.320-325.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fliermans C. B., Cherry W. B., Orrison L. H., Thacker L. Isolation of Legionella pneumophila from nonepidemic-related aquatic habitats. Appl Environ Microbiol. 1979 Jun;37(6):1239–1242. doi: 10.1128/aem.37.6.1239-1242.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ingram M., Cleary T. J., Price B. J., Price R. L., 3rd, Castro A. Rapid detection of Legionella pneumophila by flow cytometry. Cytometry. 1982 Sep;3(2):134–137. doi: 10.1002/cyto.990030212. [DOI] [PubMed] [Google Scholar]
  7. Kerker M., Van Dilla M. A., Brunsting A., Kratohvil J. P., Hsu P., Wang D. S., Gray J. W., Langlois R. G. Is the central dogma of flow cytometry true: that fluorescence intensity is proportional to cellular dye content? Cytometry. 1982 Sep;3(2):71–78. doi: 10.1002/cyto.990030202. [DOI] [PubMed] [Google Scholar]
  8. Muldrow L. L., Tyndall R. L., Fliermans C. B. Application of flow cytometry to studies of pathogenic free-living amoebae. Appl Environ Microbiol. 1982 Dec;44(6):1258–1269. doi: 10.1128/aem.44.6.1258-1269.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Orrison L. H., Bibb W. F., Cherry W. B., Thacker L. Determination of antigenic relationships among legionellae and non-legionellae by direct fluorescent-antibody and immunodiffusion tests. J Clin Microbiol. 1983 Feb;17(2):332–337. doi: 10.1128/jcm.17.2.332-337.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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