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. 1985 May;49(5):1101–1107. doi: 10.1128/aem.49.5.1101-1107.1985

Test Medium for the Growth of Nitrosomonas europaea

Chikashi Sato 1,*, Jerald L Schnoor 1, Donald B McDonald 1, Jon Huey 1
PMCID: PMC238513  PMID: 16346783

Abstract

A mineral medium for studying the growth of Nitrosomonas europaea was developed and examined. The medium was defined in terms of chemical speciation by using chemical equilibrium computer models. The medium significantly increased the metabolic activity of the organisms compared with previously developed media, yielding a specific growth rate as high as 3.0 day−1 (generation time, 5.5 h). The specific growth rate was enhanced by increasing the inoculum and was linearly correlated with the inoculum-to-total-culture volume ratio on a semilog scale. A reproducible growth rate for N. europaea was obtained with this medium under controlled experimental conditions.

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Selected References

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  1. ABRAMSON H. A., WEISS B., BARON M. O. Comparison of effect of lysergic acid diethylamide with potassium cyanide and other respiratory inhibitors on the Siamese fighting fish. Nature. 1958 Apr 19;181(4616):136–137. doi: 10.1038/1811136b0. [DOI] [PubMed] [Google Scholar]
  2. Anthonisen A. C., Loehr R. C., Prakasam T. B., Srinath E. G. Inhibition of nitrification by ammonia and nitrous acid. J Water Pollut Control Fed. 1976 May;48(5):835–852. [PubMed] [Google Scholar]
  3. ENGEL M. S., ALEXANDER M. Growth and autotrophic metabolism of Nitrosomonas europaea. J Bacteriol. 1958 Aug;76(2):217–222. doi: 10.1128/jb.76.2.217-222.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GUNDERSEN K. Preservation of nitrosomonas. Nature. 1957 Apr 13;179(4563):789–789. doi: 10.1038/179789a0. [DOI] [PubMed] [Google Scholar]
  5. LEES H. The biochemistry of the nitrifying organisms. I. The ammonia oxidizing systems of Nitrosomonas. Biochem J. 1952 Sep;52(1):134–139. doi: 10.1042/bj0520134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LEWIS R. F., PRAMER D. Isolation of Nitrosomonas in pure culture. J Bacteriol. 1958 Nov;76(5):524–528. doi: 10.1128/jb.76.5.524-528.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MEIKLEJOHN J. The isolation of Nitrosomonas europaea in pure culture. J Gen Microbiol. 1950 May;4(2):185–191. doi: 10.1099/00221287-4-2-185. [DOI] [PubMed] [Google Scholar]
  8. Soriano S., Walker N. Isolation of ammonia-oxidizing autotrophic bacteria. J Appl Bacteriol. 1968 Dec;31(4):493–497. doi: 10.1111/j.1365-2672.1968.tb00397.x. [DOI] [PubMed] [Google Scholar]
  9. Srna R. F., Baggaley A. Kinetic response of perturbed marine nitrification systems. J Water Pollut Control Fed. 1975 Mar;47(3 Pt 1):472–486. [PubMed] [Google Scholar]
  10. Tomlinson T. G., Boon A. G., Trotman C. N. Inhibition of nitrification in the activated sludge process of sewage disposal. J Appl Bacteriol. 1966 Aug;29(2):266–291. doi: 10.1111/j.1365-2672.1966.tb03477.x. [DOI] [PubMed] [Google Scholar]

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