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. 1985 May;49(5):1206–1210. doi: 10.1128/aem.49.5.1206-1210.1985

Effect of non-Legionellaceae bacteria on the multiplication of Legionella pneumophila in potable water.

R M Wadowsky, R B Yee
PMCID: PMC238530  PMID: 4004234

Abstract

A naturally occurring suspension of Legionella pneumophila and associated microbiota contained three unidentified non-Legionellaceae bacteria which supported satellite growth of a subculture of L. pneumophila on an L-cysteine-deficient medium and another bacterium which did not support growth of the subculture. Washed suspensions containing 10(3), 10(5), 10(7), or 10(8) CFU of a mixture of isolates of these non-Legionellaceae bacteria failed to support the multiplication of an isolate of agar-grown L. pneumophila which had been washed and seeded into the suspensions. The suspensions which contained 10(3), 10(5), or 10(7) CFU of the non-Legionellaceae bacteria per ml appeared to enhance survival or cryptic growth of agar-grown L. pneumophila. A decline of 1.3 log CFU of L. pneumophila per ml occurred within the first week of incubation in the sample which contained 10(8) CFU of the non-Legionellaceae bacteria per ml. In contrast to these results, naturally occurring L. pneumophila multiplied in the presence of associated microbiota. The necessity to subculture L. pneumophila and the non-Legionellaceae bacteria on artificial medium to obtain pure cultures may have affected the multiplication of L. pneumophila in tap water. Alternatively, other microorganisms may be present in the naturally occurring suspension which support the growth of this bacterium.

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Selected References

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  1. Anand C. M., Skinner A. R., Malic A., Kurtz J. B. Interaction of L. pneumophilia and a free living amoeba (Acanthamoeba palestinensis). J Hyg (Lond) 1983 Oct;91(2):167–178. doi: 10.1017/s0022172400060174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cherry W. B., Pittman B., Harris P. P., Hebert G. A., Thomason B. M., Thacker L., Weaver R. E. Detection of Legionnaires disease bacteria by direct immunofluorescent staining. J Clin Microbiol. 1978 Sep;8(3):329–338. doi: 10.1128/jcm.8.3.329-338.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FUNK H. B., KRULWICH T. A. PREPARATION OF CLEAR SILICA GELS THAT CAN BE STREAKED. J Bacteriol. 1964 Oct;88:1200–1201. doi: 10.1128/jb.88.4.1200-1201.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Favero M. S., Carson L. A., Bond W. W., Petersen N. J. Pseudomonas aeruginosa: growth in distilled water from hospitals. Science. 1971 Aug 27;173(3999):836–838. doi: 10.1126/science.173.3999.836. [DOI] [PubMed] [Google Scholar]
  5. Fields B. S., Shotts E. B., Jr, Feeley J. C., Gorman G. W., Martin W. T. Proliferation of Legionella pneumophila as an intracellular parasite of the ciliated protozoan Tetrahymena pyriformis. Appl Environ Microbiol. 1984 Mar;47(3):467–471. doi: 10.1128/aem.47.3.467-471.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. George J. R., Pine L., Reeves M. W., Harrell W. K. Amino acid requirements of Legionella pneumophila. J Clin Microbiol. 1980 Mar;11(3):286–291. doi: 10.1128/jcm.11.3.286-291.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuchta J. M., States S. J., McNamara A. M., Wadowsky R. M., Yee R. B. Susceptibility of Legionella pneumophila to chlorine in tap water. Appl Environ Microbiol. 1983 Nov;46(5):1134–1139. doi: 10.1128/aem.46.5.1134-1139.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Müller H. E. Enzymatic profile of Legionella pneumophilia. J Clin Microbiol. 1981 Mar;13(3):423–426. doi: 10.1128/jcm.13.3.423-426.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pasculle A. W., Feeley J. C., Gibson R. J., Cordes L. G., Myerowitz R. L., Patton C. M., Gorman G. W., Carmack C. L., Ezzell J. W., Dowling J. N. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J Infect Dis. 1980 Jun;141(6):727–732. doi: 10.1093/infdis/141.6.727. [DOI] [PubMed] [Google Scholar]
  10. Plouffe J. F., Webster L. R., Hackman B. Relationship between colonization of hospital building with Legionella pneumophila and hot water temperatures. Appl Environ Microbiol. 1983 Sep;46(3):769–770. doi: 10.1128/aem.46.3.769-770.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ristroph J. D., Hedlund K. W., Gowda S. Chemically defined medium for Legionella pneumophila growth. J Clin Microbiol. 1981 Jan;13(1):115–119. doi: 10.1128/jcm.13.1.115-119.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rowbotham T. J. Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J Clin Pathol. 1980 Dec;33(12):1179–1183. doi: 10.1136/jcp.33.12.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stout J., Yu V. L., Vickers R. M., Zuravleff J., Best M., Brown A., Yee R. B., Wadowsky R. Ubiquitousness of Legionella pneumophila in the water supply of a hospital with endemic Legionnaires' disease. N Engl J Med. 1982 Feb 25;306(8):466–468. doi: 10.1056/NEJM198202253060807. [DOI] [PubMed] [Google Scholar]
  14. Tesh M. J., Miller R. D. Amino acid requirements for Legionella pneumophila growth. J Clin Microbiol. 1981 May;13(5):865–869. doi: 10.1128/jcm.13.5.865-869.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thompson M. R., Miller R. D., Iglewski B. H. In vitro production of an extracellular protease by Legionella pneumophila. Infect Immun. 1981 Oct;34(1):299–302. doi: 10.1128/iai.34.1.299-302.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tison D. L., Pope D. H., Cherry W. B., Fliermans C. B. Growth of Legionella pneumophila in association with blue-green algae (cyanobacteria). Appl Environ Microbiol. 1980 Feb;39(2):456–459. doi: 10.1128/aem.39.2.456-459.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tobin J. O., Bartlett C. L., Waitkins S. A., Barrow G. I., Macrae A. D., Taylor A. G., Fallon R. J., Lynch F. R. Legionnaires' disease: further evidence to implicate water storage and distribution systems as sources. Br Med J (Clin Res Ed) 1981 Feb 14;282(6263):573–573. doi: 10.1136/bmj.282.6263.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tyndall R. L., Domingue E. L. Cocultivation of Legionella pneumophila and free-living amoebae. Appl Environ Microbiol. 1982 Oct;44(4):954–959. doi: 10.1128/aem.44.4.954-959.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wadowsky R. M., Wolford R., McNamara A. M., Yee R. B. Effect of temperature, pH, and oxygen level on the multiplication of naturally occurring Legionella pneumophila in potable water. Appl Environ Microbiol. 1985 May;49(5):1197–1205. doi: 10.1128/aem.49.5.1197-1205.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wadowsky R. M., Yee R. B. Glycine-containing selective medium for isolation of Legionellaceae from environmental specimens. Appl Environ Microbiol. 1981 Nov;42(5):768–772. doi: 10.1128/aem.42.5.768-772.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wadowsky R. M., Yee R. B., Mezmar L., Wing E. J., Dowling J. N. Hot water systems as sources of Legionella pneumophila in hospital and nonhospital plumbing fixtures. Appl Environ Microbiol. 1982 May;43(5):1104–1110. doi: 10.1128/aem.43.5.1104-1110.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wadowsky R. M., Yee R. B. Satellite growth of Legionella pneumophila with an environmental isolate of Flavobacterium breve. Appl Environ Microbiol. 1983 Dec;46(6):1447–1449. doi: 10.1128/aem.46.6.1447-1449.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yee R. B., Wadowsky R. M. Multiplication of Legionella pneumophila in unsterilized tap water. Appl Environ Microbiol. 1982 Jun;43(6):1330–1334. doi: 10.1128/aem.43.6.1330-1334.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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