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. 1985 Jul;50(1):152–159. doi: 10.1128/aem.50.1.152-159.1985

Regulation of β-1, 4-Glucosidase Expression by Candida wickerhamii

S N Freer 1,*, R W Detroy 1
PMCID: PMC238588  PMID: 16346833

Abstract

Candida wickerhamii NRRL Y-2563 expressed β-glucosidase activity (3 to 8 U/ml) constitutively when grown aerobically in complex medium containing either glycerol, succinate, xylose, galactose, or cellobiose as the carbon source. The addition of a high concentration of glucose (>75 g/liter) repressed β-glucosidase expression (<0.3 U/ml); however, this yeast did produce β-glucosidase when the initial glucose concentration was ≤50 g/liter. When grown aerobically in medium containing glucose plus the above-listed carbon sources, diauxic utilization of the carbon source was observed and the expression of β-glucosidase was glucose repressed. Surprisingly, glucose repression did not occur when the cells were grown anaerobically. When grown anaerobically in medium containing 100 g of glucose per liter, C. wickerhamii produced 6 to 9 U of enzyme per ml and did not demonstrate diauxic utilization of glucose-cellobiose mixtures. To our knowledge, this is the first report of apparent derepression of a glucose-repressed enzyme by anaerobiosis.

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Selected References

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  1. Berg B., Pettersson G. Location and formation of cellulases in Trichoderma viride. J Appl Bacteriol. 1977 Feb;42(1):65–75. doi: 10.1111/j.1365-2672.1977.tb00670.x. [DOI] [PubMed] [Google Scholar]
  2. Chu F. K., Maley F. The effect of glucose on the synthesis and glycosylation of the polypeptide moiety of yeast external invertase. J Biol Chem. 1980 Jul 10;255(13):6392–6397. [PubMed] [Google Scholar]
  3. Deshpande V., Eriksson K. E., Pettersson B. Production , purification and partial characterization of 1,4-beta-glucosidase enzymes from Sporotrichum pulverulentum. Eur J Biochem. 1978 Sep 15;90(1):191–198. doi: 10.1111/j.1432-1033.1978.tb12590.x. [DOI] [PubMed] [Google Scholar]
  4. Desrochers M., Jurasek L., Paice M. G. High Production of beta-Glucosidase in Schizophyllum commune: Isolation of the Enzyme and Effect of the Culture Filtrate on Cellulose Hydrolysis. Appl Environ Microbiol. 1981 Jan;41(1):222–228. doi: 10.1128/aem.41.1.222-228.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gascón S., Lampen J. O. Purification of the internal invertase of yeast. J Biol Chem. 1968 Apr 10;243(7):1567–1572. [PubMed] [Google Scholar]
  6. Gascón S., Neumann N. P., Lampen J. O. Comparative study of the properties of the purified internal and external invertases from yeast. J Biol Chem. 1968 Apr 10;243(7):1573–1577. [PubMed] [Google Scholar]
  7. HERMAN A., HALVORSON H. GENETIC CONTROL OF BETA-GLUCOSIDASE SYNTHESIS IN SACCHAROMYCES LACTIS. J Bacteriol. 1963 Apr;85:901–910. doi: 10.1128/jb.85.4.901-910.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HERMAN A., HALVORSON H. IDENTIFICATION OF THE STRUCTURAL GENE FOR BETA-GLUCOSIDASE IN SACCHAROMYCES LACTIS. J Bacteriol. 1963 Apr;85:895–900. doi: 10.1128/jb.85.4.895-900.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Halliwell G., Griffin M. The nature and mode of action of the cellulolytic component C1 of Trichoderma koningii on native cellulose. Biochem J. 1973 Dec;135(4):587–594. doi: 10.1042/bj1350587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ladisch M. R., Hong J., Voloch M., Tsao G. T. Cellulase kinetics. Basic Life Sci. 1981;18:55–83. doi: 10.1007/978-1-4684-3980-9_5. [DOI] [PubMed] [Google Scholar]
  11. Lloyd J. B., Whelan W. J. An improved method for enzymic determination of glucose in the presence of maltose. Anal Biochem. 1969 Sep;30(3):467–470. doi: 10.1016/0003-2697(69)90143-2. [DOI] [PubMed] [Google Scholar]
  12. MACQUILLAN A. M., HALVORSON H. O. Metabolic control of beta-glucosidase synthesis in yeast. J Bacteriol. 1962 Jul;84:23–30. doi: 10.1128/jb.84.1.23-30.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Perlman D., Halvorson H. O. Distinct repressible mRNAs for cytoplasmic and secreted yeast invertase are encoded by a single gene. Cell. 1981 Aug;25(2):525–536. doi: 10.1016/0092-8674(81)90071-4. [DOI] [PubMed] [Google Scholar]
  14. Smith M. H., Gold M. H. Phanerochaete chrysosporium beta-Glucosidases: Induction, Cellular Localization, and Physical Characterization. Appl Environ Microbiol. 1979 May;37(5):938–942. doi: 10.1128/aem.37.5.938-942.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sternberg D. Beta-glucosidase of Trichoderma: its biosynthesis and role in saccharification of cellulose. Appl Environ Microbiol. 1976 May;31(5):648–654. doi: 10.1128/aem.31.5.648-654.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sternberg D., Vijayakumar P., Reese E. T. beta-Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose. Can J Microbiol. 1977 Feb;23(2):139–147. doi: 10.1139/m77-020. [DOI] [PubMed] [Google Scholar]
  17. Van Wijk R., Ouwehand J., van den Bos T., Koningsberger V. V. Induction and catabolite repression of alpha-glucosidase synthesis in protoplasts of Saccharomyces carlsbergensis. Biochim Biophys Acta. 1969 Jul 22;186(1):178–191. doi: 10.1016/0005-2787(69)90501-2. [DOI] [PubMed] [Google Scholar]
  18. Wilson R. W., Niederpruem D. J. Control of beta-glucosidases in schizophyllum commune. Can J Microbiol. 1967 Aug;13(8):1009–1020. doi: 10.1139/m67-135. [DOI] [PubMed] [Google Scholar]
  19. Wood T. M., McCrae S. I. Cellulase from Fusarium solani: purification and properties of the C1 component. Carbohydr Res. 1977 Aug;57:117–133. doi: 10.1016/s0008-6215(00)81925-4. [DOI] [PubMed] [Google Scholar]
  20. Wood T. M., McCrae S. I., Macfarlane C. C. The isolation, purification and properties of the cellobiohydrolase component of Penicillium funiculosum cellulase. Biochem J. 1980 Jul 1;189(1):51–65. doi: 10.1042/bj1890051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wood T. M., McCrae S. I. The cellulase of Trichoderma koningii. Purification and properties of some endoglucanase components with special reference to their action on cellulose when acting alone and in synergism with the cellobiohydrolase. Biochem J. 1978 Apr 1;171(1):61–72. doi: 10.1042/bj1710061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wood T. M. The cellulase of Fusarium solani. Purification and specificity of the -(1-4)-glucanase and the -D-glucosidase components. Biochem J. 1971 Feb;121(3):353–362. doi: 10.1042/bj1210353. [DOI] [PMC free article] [PubMed] [Google Scholar]

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