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. 1985 Jul;50(1):169–171. doi: 10.1128/aem.50.1.169-171.1985

Construction of a cosmid clone library of Pseudomonas syringae pv. phaseolicola and isolation of genes by functional complementation.

M Ehrenshaft, D Mills
PMCID: PMC238591  PMID: 2992375

Abstract

A genomic library constructed from a wild-type strain of Pseudomonas syringae pv. phaseolicola in the broad-host-range cosmid vector pVK102 was used to isolate wild-type genes by complementation of Tn5-induced auxotrophic mutants. Selection pressure was required for maintenance of the vector and members of the library in strains of P. syringae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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