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. 1985 Aug;50(2):523–526. doi: 10.1128/aem.50.2.523-526.1985

Membrane-associated viral complexes observed in stools and cell culture.

F P Williams Jr
PMCID: PMC238652  PMID: 2996424

Abstract

Viral complexes observed to be membrane associated rather than clumped by antibody were detected in a rotavirus-containing stool specimen by negative-stain electron microscopy. These "viral packets" were also observed in cell culture fluids after repeated passaging and contained up to 100 virions. Other stool specimens have been observed to contain similar packets of parvovirus-like particles. Such complexes must be expected in fecally contaminated water.

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Selected References

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  1. Almeida J. D., Hall T., Banatvala J. E., Totterdell B. M., Chrystie I. L. The effect of trypsin on the growth of rotavirus. J Gen Virol. 1978 Jul;40(1):213–218. doi: 10.1099/0022-1317-40-1-213. [DOI] [PubMed] [Google Scholar]
  2. Altenburg B. C., Graham D. Y., Estes M. K. Ultrastructural study of rotavirus replication in cultured cells. J Gen Virol. 1980 Jan;46(1):75–85. doi: 10.1099/0022-1317-46-1-75. [DOI] [PubMed] [Google Scholar]
  3. Benton W. H., Ward R. L. Induction of cytopathogenicity in mammalian cell lines challenged with culturable enteric viruses and its enhancement by 5-iododeoxyuridine. Appl Environ Microbiol. 1982 Apr;43(4):861–868. doi: 10.1128/aem.43.4.861-868.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berman D., Hoff J. C. Inactivation of simian rotavirus SA11 by chlorine, chlorine dioxide, and monochloramine. Appl Environ Microbiol. 1984 Aug;48(2):317–323. doi: 10.1128/aem.48.2.317-323.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blacklow N. R., Cukor G., Bedigian M. K., Echeverria P., Greenberg H. B., Schreiber D. S., Trier J. S. Immune response and prevalence of antibody to Norwalk enteritis virus as determined by radioimmunoassay. J Clin Microbiol. 1979 Dec;10(6):903–909. doi: 10.1128/jcm.10.6.903-909.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gbewonyo A. J. Rapid and reliable method for diagnostic electron microscopy of faeces. J Microsc. 1982 May;126(Pt 2):191–195. doi: 10.1111/j.1365-2818.1982.tb00371.x. [DOI] [PubMed] [Google Scholar]
  7. Harakeh M., Butler M. Inactivation of human rotavirus, SA11 and other enteric viruses in effluent by disinfectants. J Hyg (Lond) 1984 Aug;93(1):157–163. doi: 10.1017/s0022172400061040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hopkins R. S., Gaspard G. B., Williams F. P., Jr, Karlin R. J., Cukor G., Blacklow N. R. A community waterborne gastroenteritis outbreak: evidence for rotavirus as the agent. Am J Public Health. 1984 Mar;74(3):263–265. doi: 10.2105/ajph.74.3.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Infantile enteritis viruses: morphogenesis and morphology. J Virol. 1975 Oct;16(4):937–943. doi: 10.1128/jvi.16.4.937-943.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Korolev M. B., Khaustov V. I., Shekoian L. A. Morfogenez rotavirusa cheloveka v kul'ture kletok. Vopr Virusol. 1981 May-Jun;(3):309–315. [PubMed] [Google Scholar]
  11. Narang H. K., Codd A. A. Frequency of preclumped virus in routine fecal specimens from patients with acute nonbacterial gastroenteritis. J Clin Microbiol. 1981 May;13(5):982–988. doi: 10.1128/jcm.13.5.982-988.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Saif L. J., Theil K. W., Bohl E. H. Morphogenesis of porcine rotavirus in porcine kidney cell cultures and intestinal epithelial cells. J Gen Virol. 1978 May;39(2):205–217. doi: 10.1099/0022-1317-39-2-205. [DOI] [PubMed] [Google Scholar]
  13. Sharp D. G., Floyd R., Johnson J. D. Initial fast reaction of bromine on reovirus in turbulent flowing water. Appl Environ Microbiol. 1976 Feb;31(2):173–181. doi: 10.1128/aem.31.2.173-181.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sharp D. G., Floyd R., Johnson J. D. Nature of the surviving plaque-forming unit of reovirus in water containing bromine. Appl Microbiol. 1975 Jan;29(1):94–101. doi: 10.1128/am.29.1.94-101.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Suzuki H., Konno T., Kitaoka S., Sato T., Ebina T., Ishida N. Further observations on the morphogenesis of human rotavirus in MA 104 cells. Arch Virol. 1984;79(3-4):147–159. doi: 10.1007/BF01310808. [DOI] [PubMed] [Google Scholar]
  16. Suzuki H., Kutsuzawa T., Konno T., Ebina T., Ishida N. Morphogenesis of human rotavirus type 2 Wa strain in MA 104 cells. Arch Virol. 1981;70(1):33–41. doi: 10.1007/BF01320791. [DOI] [PubMed] [Google Scholar]
  17. Young D. C., Sharp D. G. Poliovirus aggregates and their survival in water. Appl Environ Microbiol. 1977 Jan;33(1):168–177. doi: 10.1128/aem.33.1.168-177.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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