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. 1986 Feb;51(2):307–311. doi: 10.1128/aem.51.2.307-311.1986

Inactivation of Campylobacter jejuni by chlorine and monochloramine.

M J Blaser, P F Smith, W L Wang, J C Hoff
PMCID: PMC238864  PMID: 3954344

Abstract

Campylobacter jejuni and closely related organisms are important bacterial causes of acute diarrheal illness in the United States. Both endemic and epidemic infections have been associated with consuming untreated or improperly treated surface water. We compared susceptibility of three C. jejuni strains and Escherichia coli ATCC 11229 with standard procedures used to disinfect water. Inactivation of bacterial preparations with 0.1 mg of chlorine and 1.0 mg of monochloramine per liter was determined at pH 6 and 8 and at 4 and 25 degrees C. Under virtually every condition tested, each of the three C. jejuni strains was more susceptible than the E. coli control strain, with greater than 99% inactivation after 15 min of contact with 1.0 mg of monochloramine per liter or 5 min of contact with 0.1 mg of free chlorine per liter. Results of experiments in which an antibiotic-containing medium was used suggest that a high proportion of the remaining cells were injured. An animal-passaged C. jejuni strain was as susceptible to chlorine disinfection as were laboratory-passaged strains. These results suggest that disinfection procedures commonly used for treatment of drinking water to remove coliform bacteria are adequate to eliminate C. jejuni and further correlate with the absence of outbreaks associated with properly treated water.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berman D., Hoff J. C. Inactivation of simian rotavirus SA11 by chlorine, chlorine dioxide, and monochloramine. Appl Environ Microbiol. 1984 Aug;48(2):317–323. doi: 10.1128/aem.48.2.317-323.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blaser M. J., Duncan D. J., Warren G. H., Wang W. L. Experimental Campylobacter jejuni infection of adult mice. Infect Immun. 1983 Feb;39(2):908–916. doi: 10.1128/iai.39.2.908-916.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blaser M. J., Hardesty H. L., Powers B., Wang W. L. Survival of Campylobacter fetus subsp. jejuni in biological milieus. J Clin Microbiol. 1980 Apr;11(4):309–313. doi: 10.1128/jcm.11.4.309-313.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blaser M. J., Reller L. B. Campylobacter enteritis. N Engl J Med. 1981 Dec 10;305(24):1444–1452. doi: 10.1056/NEJM198112103052404. [DOI] [PubMed] [Google Scholar]
  5. Blaser M. J., Taylor D. N., Feldman R. A. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev. 1983;5:157–176. doi: 10.1093/oxfordjournals.epirev.a036256. [DOI] [PubMed] [Google Scholar]
  6. Blaser M. J., Wells J. G., Feldman R. A., Pollard R. A., Allen J. R. Campylobacter enteritis in the United States. A multicenter study. Ann Intern Med. 1983 Mar;98(3):360–365. doi: 10.7326/0003-4819-98-3-360. [DOI] [PubMed] [Google Scholar]
  7. McFeters G. A., Cameron S. C., LeChevallier M. W. Influence of diluents, media, and membrane filters on detection fo injured waterborne coliform bacteria. Appl Environ Microbiol. 1982 Jan;43(1):97–103. doi: 10.1128/aem.43.1.97-103.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Penner J. L., Hennessy J. N. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol. 1980 Dec;12(6):732–737. doi: 10.1128/jcm.12.6.732-737.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Peterson D. A., Hurley T. R., Hoff J. C., Wolfe L. G. Effect of chlorine treatment on infectivity of hepatitis A virus. Appl Environ Microbiol. 1983 Jan;45(1):223–227. doi: 10.1128/aem.45.1.223-227.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Taylor D. N., McDermott K. T., Little J. R., Wells J. G., Blaser M. J. Campylobacter enteritis from untreated water in the Rocky Mountains. Ann Intern Med. 1983 Jul;99(1):38–40. doi: 10.7326/0003-4819-99-1-38. [DOI] [PubMed] [Google Scholar]
  11. Wang W. L., Powers B. W., Leuchtefeld N. W., Blaser M. J. Effects of disinfectants on Campylobacter jejuni. Appl Environ Microbiol. 1983 Apr;45(4):1202–1205. doi: 10.1128/aem.45.4.1202-1205.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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