Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 Apr;51(4):868–871. doi: 10.1128/aem.51.4.868-871.1986

Asymbiotic Acetylene Reduction by a Fast-Growing Cowpea Rhizobium Strain with Nitrogenase Structural Genes Located on a Symbiotic Plasmid

Gregory L Bender 1, Jacek Plazinski 1, Barry G Rolfe 1,*
PMCID: PMC238976  PMID: 16347045

Abstract

A procedure was designed which enabled the detection of ex planta nitrogenase activity in the fast-growing cowpea Rhizobium strain IHP100. Nitrogenase activity in agar culture under air occurred at a rate similar to that found for Bradyrhizobium strain CB756 but lower than that for Rhizobium strain ORS571. Hybridization studies showed that both nod and nif genes were located on a 410-kilobase Sym plasmid in strain IHP100.

Full text

PDF
868

Images in this article

869Image
on p.869

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cen Y., Bender G. L., Trinick M. J., Morrison N. A., Scott K. F., Gresshoff P. M., Shine J., Rolfe B. G. Transposon mutagenesis in rhizobia which can nodulate both legumes and the nonlegume parasponia. Appl Environ Microbiol. 1982 Jan;43(1):233–236. doi: 10.1128/aem.43.1.233-236.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dreyfus B. L., Elmerich C., Dommergues Y. R. Free-living Rhizobium strain able to grow on n(2) as the sole nitrogen source. Appl Environ Microbiol. 1983 Feb;45(2):711–713. doi: 10.1128/aem.45.2.711-713.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gibson A. H., Scowcroft W. R., Child J. J., Pagan J. D. Nitrogenase activity in cultured Rhizobium sp. strain 32H1: nutritional and physical considerations. Arch Microbiol. 1976 May 3;108(1):45–54. doi: 10.1007/BF00425092. [DOI] [PubMed] [Google Scholar]
  4. Keister D. L. Acetylene reduction by pure cultures of Rhizobia. J Bacteriol. 1975 Sep;123(3):1265–1268. doi: 10.1128/jb.123.3.1265-1268.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Keister D. L., Evans W. R. Oxygen requirement for acetylene reduction by pure cultures of rhizobia. J Bacteriol. 1976 Jul;127(1):149–153. doi: 10.1128/jb.127.1.149-153.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kennedy L. D., Pankhurst C. E. Nitrogenase activity of Rhizobium sp. strains in pure culture in relation to extracellular polysaccharide composition and antigenic affinity. Microbios. 1978;23(93-94):167–173. [PubMed] [Google Scholar]
  7. Plazinski J., Cen Y. H., Rolfe B. G. General method for the identification of plasmid species in fast-growing soil microorganisms. Appl Environ Microbiol. 1985 Apr;49(4):1001–1003. doi: 10.1128/aem.49.4.1001-1003.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sciaky D., Montoya A. L., Chilton M. D. Fingerprints of Agrobacterium Ti plasmids. Plasmid. 1978 Feb;1(2):238–253. doi: 10.1016/0147-619x(78)90042-2. [DOI] [PubMed] [Google Scholar]
  9. Scowcroft W. R., Gibson A. H., Pagan J. D. Nitrogen fixation in cultured cowpea Rhizobia: inhibition and regulation of nitrogenase activity. Biochem Biophys Res Commun. 1976 Nov 22;73(2):516–523. doi: 10.1016/0006-291x(76)90737-3. [DOI] [PubMed] [Google Scholar]
  10. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  11. Tjepkema J., Evans H. J. Nitrogen fixation by free-living Rhizobium in a defined liquid medium. Biochem Biophys Res Commun. 1975 Jul 22;65(2):625–628. doi: 10.1016/s0006-291x(75)80192-6. [DOI] [PubMed] [Google Scholar]
  12. Weinman J. J., Fellows F. F., Gresshoff P. M., Shine J., Scott K. F. Structural analysis of the genes encoding the molybdenum-iron protein of nitrogenase in the Parasponia rhizobium strain ANU289. Nucleic Acids Res. 1984 Nov 26;12(22):8329–8344. doi: 10.1093/nar/12.22.8329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Whitfeld P. L., Seeburg P. H., Shine J. The human pro-opiomelanocortin gene: organization, sequence, and interspersion with repetitive DNA. DNA. 1982;1(2):133–143. doi: 10.1089/dna.1.1982.1.133. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES