Abstract
The transformation of 22-hydroxy-23,24-bisnorchol-4-en-3-one to 7α-22-dihydroxy-23,24-bisnorchol-4-en-3-one by Botryodiploida theobromae, Lasiodiplodia theobromae, and various Botryosphaeria strains is described. Factors affecting the reaction were incubation temperature, sonication of the substrate, and addition of 2,2′-dipyridyl, extra carbohydrate, and Amberlite XAD-7. The enzyme responsible for the reaction appeared to be very specific and was not characteristic of all members of the genera listed above.
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Selected References
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- Abul-Hajj Y. J. Microbiological hydroxylation of 4-androstene-3,17-dione. Lloydia. 1970 Jun;33(2):278–278. [PubMed] [Google Scholar]
- Defaye G., Luche M. J., Chambaz E. M. Microbiological 7- and 15-hydroxylations of C-19 steroids. J Steroid Biochem. 1978 Apr;9(4):331–336. doi: 10.1016/0022-4731(78)90626-x. [DOI] [PubMed] [Google Scholar]
- Howe R., Moore R. H. Microbiological reduction of 2-methyl-1,2-di-3-pyridyl-1-propanone (metyrapone). J Med Chem. 1971 Apr;14(4):287–288. doi: 10.1021/jm00286a004. [DOI] [PubMed] [Google Scholar]
- Howe R., Moore R. H., Rao B. S., Wood A. H. Metabolism of 2-(4-chlorophenyl)thiazol-4-ylacetic acid (fenclozic acid) and related compounds by microorganisms. J Med Chem. 1972 Oct;15(10):1040–1045. doi: 10.1021/jm00280a012. [DOI] [PubMed] [Google Scholar]
- Leppik R. A., Park R. J., Smith M. G. Aerobic catabolism of bile acids. Appl Environ Microbiol. 1982 Oct;44(4):771–776. doi: 10.1128/aem.44.4.771-776.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singh K., Sehgal S. N., Vézina C. Transformation of steroids by Mucor griseo-cyanus. Can J Microbiol. 1967 Sep;13(9):1271–1281. doi: 10.1139/m67-172. [DOI] [PubMed] [Google Scholar]
- Wix G., Büki K. G., Tömörkény E., Ambrus G. Inhibition of steroid nucleus degradation in mycobacterial transformations. Steroids. 1968 Mar;11(3):401–413. doi: 10.1016/s0039-128x(68)80150-3. [DOI] [PubMed] [Google Scholar]