Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 May;51(5):1121–1123. doi: 10.1128/aem.51.5.1121-1123.1986

Phosphotransferase Activity in Clostridium acetobutylicum from Acidogenic and Solventogenic Phases of Growth

Robert W Hutkins 1, Eva R Kashket 1,*
PMCID: PMC239020  PMID: 16347058

Abstract

Clostridium acetobutylicum cells, when energized with fructose, transported and phosphorylated the glucose analog 2-deoxyglucose by a phosphoenolpyruvate-dependent phosphotransferase (PT) system. Butanol up to 2% did not inhibit PT activity, although its chaotropic effect on the cell membrane caused cellular phosphoenolpyruvate and the 2-deoxyglucose-6-phosphate to leak out. Cells harvested from the solventogenic phase of batch growth had a significantly lower PT activity than did cells from the acidogenic phase.

Full text

PDF
1121

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baronofsky J. J., Schreurs W. J., Kashket E. R. Uncoupling by Acetic Acid Limits Growth of and Acetogenesis by Clostridium thermoaceticum. Appl Environ Microbiol. 1984 Dec;48(6):1134–1139. doi: 10.1128/aem.48.6.1134-1139.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Booth I. R., Morris J. G. Carbohydrate transport in Clostridium pasteurianum. Biosci Rep. 1982 Jan;2(1):47–53. doi: 10.1007/BF01142198. [DOI] [PubMed] [Google Scholar]
  3. Bowles L. K., Ellefson W. L. Effects of butanol on Clostridium acetobutylicum. Appl Environ Microbiol. 1985 Nov;50(5):1165–1170. doi: 10.1128/aem.50.5.1165-1170.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dills S. S., Apperson A., Schmidt M. R., Saier M. H., Jr Carbohydrate transport in bacteria. Microbiol Rev. 1980 Sep;44(3):385–418. doi: 10.1128/mr.44.3.385-418.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Groves D. J., Gronlund A. F. Carbohydrate transport in Clostridium perfringens type A. J Bacteriol. 1969 Dec;100(3):1256–1263. doi: 10.1128/jb.100.3.1256-1263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Herrero A. A., Gomez R. F., Roberts M. F. 31P NMR studies of Clostridium thermocellum. Mechanism of end product inhibition by ethanol. J Biol Chem. 1985 Jun 25;260(12):7442–7451. [PubMed] [Google Scholar]
  7. Kashket E. R., Barker S. L. Effects of potassium ions on the electrical and pH gradients across the membrane of Streptococcus lactis cells. J Bacteriol. 1977 Jun;130(3):1017–1023. doi: 10.1128/jb.130.3.1017-1023.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lovitt R. W., Longin R., Zeikus J. G. Ethanol Production by Thermophilic Bacteria: Physiological Comparison of Solvent Effects on Parent and Alcohol-Tolerant Strains of Clostridium thermohydrosulfuricum. Appl Environ Microbiol. 1984 Jul;48(1):171–177. doi: 10.1128/aem.48.1.171-177.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ounine K., Petitdemange H., Raval G., Gay R. Regulation and butanol inhibition of D-xylose and D-glucose uptake in Clostridium acetobutylicum. Appl Environ Microbiol. 1985 Apr;49(4):874–878. doi: 10.1128/aem.49.4.874-878.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Patni N. J., Alexander J. K. Catabolism of fructose and mannitol in Clostridium thermocellum: presence of phosphoenolpyruvate: fructose phosphotransferase, fructose 1-phosphate kinase, phosphoenolpyruvate: mannitol phosphotransferase, and mannitol 1-phosphate dehydrogenase in cell extracts. J Bacteriol. 1971 Jan;105(1):226–231. doi: 10.1128/jb.105.1.226-231.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Zeikus J. G. Chemical and fuel production by anaerobic bacteria. Annu Rev Microbiol. 1980;34:423–464. doi: 10.1146/annurev.mi.34.100180.002231. [DOI] [PubMed] [Google Scholar]
  12. von Hugo H., Gottschalk G. Distribution of 1-phosphofructokinase and PEP:fructose phosphotransferase activity in Clostridia. FEBS Lett. 1974 Sep 15;46(1):106–108. doi: 10.1016/0014-5793(74)80345-5. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES