Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 May;51(5):1138–1140. doi: 10.1128/aem.51.5.1138-1140.1986

A simple and generally applicable procedure for releasing DNA from bacterial cells.

L S Heath, G L Sloan, H E Heath
PMCID: PMC239025  PMID: 3524454

Abstract

Treatment of Staphylococcus simulans biovar staphylolyticus cells with acetone before digestion with lysozyme made the cells susceptible to lysis by sodium dodecyl sulfate. This technique was found to be useful for releasing DNA from a wide variety of gram-positive and gram-negative organisms.

Full text

PDF
1138

Images in this article

1139Image
on p.1139
1140Image
on p.1140
1140Image
on p.1140

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhaduri S., Demchick P. H. Simple and rapid method for disruption of bacteria for protein studies. Appl Environ Microbiol. 1983 Oct;46(4):941–943. doi: 10.1128/aem.46.4.941-943.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnboim H. C. A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol. 1983;100:243–255. doi: 10.1016/0076-6879(83)00059-2. [DOI] [PubMed] [Google Scholar]
  3. Blin N., Stafford D. W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976 Sep;3(9):2303–2308. doi: 10.1093/nar/3.9.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dyer D. W., Iandolo J. J. Rapid isolation of DNA from Staphylococcus aureus. Appl Environ Microbiol. 1983 Jul;46(1):283–285. doi: 10.1128/aem.46.1.283-285.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klesius P. H., Schuhardt V. T. Use of lysostaphin in the isolation of highly polymerized deoxyribonucleic acid and in the taxonomy of aerobic Micrococcaceae. J Bacteriol. 1968 Mar;95(3):739–743. doi: 10.1128/jb.95.3.739-743.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Loening U. E. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation. Biochem J. 1969 Jun;113(1):131–138. doi: 10.1042/bj1130131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  8. Morse M. L., Hill K. L., Egan J. B., Hengstenberg W. Metabolism of lactose by Staphylococcus aureus and its genetic basis. J Bacteriol. 1968 Jun;95(6):2270–2274. doi: 10.1128/jb.95.6.2270-2274.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Novick R., Zouzias D., Rush M. Nucleic acid hybridization analysis of an integrated plasmid in Staphylococcus aureus. J Bacteriol. 1975 Dec;124(3):1424–1428. doi: 10.1128/jb.124.3.1424-1428.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Olsen R. H., DeBusscher G., McCombie W. R. Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol. 1982 Apr;150(1):60–69. doi: 10.1128/jb.150.1.60-69.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Robinson J. M., Hardman J. K., Sloan G. L. Relationship between lysostaphin endopeptidase production and cell wall composition in Staphylococcus staphylolyticus. J Bacteriol. 1979 Mar;137(3):1158–1164. doi: 10.1128/jb.137.3.1158-1164.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rush M., Novick R., DeLap R. Detection and quantitation of Staphylococcus aureus penicillinase plasmid deoxyribonucleic acid by reassociation kinetics. J Bacteriol. 1975 Dec;124(3):1417–1423. doi: 10.1128/jb.124.3.1417-1423.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES