Abstract
Rehydration and equilibration (4 h) of feeds and feed ingredients at water/sample ratios (vol/wt) of 1.4 to 3.2 did not markedly increase recovery of Salmonella spp. in the slurry when analyzed by standard cultural and direct enrichment methods. Of 143 naturally contaminated samples examined, equilibration increased levels of detection from 106 to 109 positive samples by the standard cultural method and from 103 to 112 by direct enrichment. Results suggest that nonhomogeneous distribution of low incident numbers of salmonellae in test samples rather than an equilibration-dependent response provided for the observed heterogeneity in recovery patterns. The novel equilibration approach is of limited application and requires validation on an individual food basis.
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Selected References
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- Beckers H. J., van Leusden F. M., Roberts D., Pietzsch O., Price T. H., van Schothorst M., Tips P. D., Vassiliadis P., Kampelmacher E. H. Collaborative study on the isolation of salmonella from artificially contaminated milk powder. J Appl Bacteriol. 1985 Jul;59(1):35–40. doi: 10.1111/j.1365-2672.1985.tb01772.x. [DOI] [PubMed] [Google Scholar]
- Gabis D. A., Silliker J. H. ICMSF methods studies. VI. The influence of selective enrichment media and incubation temperatures on the detection of Salmonella in dried foods and feeds. Can J Microbiol. 1974 Nov;20(11):1509–1511. doi: 10.1139/m74-235. [DOI] [PubMed] [Google Scholar]
- Poelma P. L., Andrews W. H., Wilson C. R., Bennett B., Elliot P. H., Estela L. A., Flowers R., Jogan M. J., Lancette G. A., Liddle K. Recovery of Salmonella species from nonfat dry milk rehydrated under rapid and reduced pre-enrichment conditions: collaborative study. J Assoc Off Anal Chem. 1984 Jul-Aug;67(4):807–810. [PubMed] [Google Scholar]
- Ray B., Jezeski J. J., Busta F. F. Effect of rehydration on recovery, repair, and growth of injured freeze-dried Salmonella anatum. Appl Microbiol. 1971 Aug;22(2):184–189. doi: 10.1128/am.22.2.184-189.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]